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http://www.addgene.org/165554

Species: Other
Genetic Insert: T4-DNA Ligase
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Ligase derived from T4 bacteriophage expressed in the early stage of lytic development. Has been implicated in T4 DNA synthesis and genetic recombination. May also play a role in T4 DNA repair. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165554 Copy   

•   Source: Addgene

http://www.addgene.org/165555

Species: Other
Genetic Insert: CIP (calf intestinal phosphatase)
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: CIAP reporter; can be used in spectrophotometric or luminescence assay when appropriate substrate is used. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165555 Copy   

•   Source: Addgene

http://www.addgene.org/165559

Species: Other
Genetic Insert: T5 Exonuclease
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Double-stranded DNA specific exonuclease and single-stranded DNA endonuclease. Initiates at the 5' termini of linear or nicked double-stranded DNA. Cleaves linear or nicked double-stranded DNA in the 5' to 3' direction. Ideal for removal of incomplete ligation products from ligated circular double-stranded DNA, degradation of denatured DNA from alkaline-based plasmid purification methods for improved DNA cloning, and degradation of contaminating linear and nicked DNA in plasmid samples. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165559 Copy   

•   Source: Addgene

http://www.addgene.org/165580

Species: Other
Genetic Insert: Bovine DNTT
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Adds dT at the end of PCR blunted DNA fragments/vectors that can then be used in TOPO cloning; Addition of homopolymer tails to the 3' ends of DNA; Labeling the 3' ends of DNA with modified nucleotides (e.g., ddNTP, DIG-dUTP); TUNEL assay (in situ localization of apoptosis); TdT dependent PCR. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165580 Copy   

•   Source: Addgene

http://www.addgene.org/165574

Species: Other
Genetic Insert: DNA Ligase III
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Repairs single strand breaks in DNA efficiently. Unable to perform either blunt-end joining or AMP- dependent relaxation of supercoiled DNA (Elder, R.H. et al . , Bur. J. Biochem.. 203:53-58 (1992)). The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165574 Copy   

•   Source: Addgene

http://www.addgene.org/165573

Species: Other
Genetic Insert: phi29 DNA Polymerase
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: DNA polymerase responsible for protein-primed viral DNA replication by strand displacement with high processivity and fidelity. Possesses three enzymatic activities: DNA synthesis (polymerase), primer terminal protein (TP) deoxynucleotidylation, and 3' to 5' exonuclease activity. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165573 Copy   

•   Source: Addgene

http://www.addgene.org/165578

Species: Other
Genetic Insert: M.NcoI
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Site-specific DNA methyltransferase; protects DNA from cleavage by NcoI. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165578 Copy   

•   Source: Addgene

http://www.addgene.org/165570

Species: Other
Genetic Insert: P45
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Significantly improves maximum amplicon size and PCR yields for Family B polymerases like Pfu or Phusion. Comparable to ArchaeMaxx/PfuTurbo. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165570 Copy   

•   Source: Addgene

http://www.addgene.org/165571

Species: Other
Genetic Insert: Exonuclease III E. coli
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Double-stranded DNA specific exonuclease. Initiates at the 3' termini of linear double-stranded DNA with 5' overhangs or blunt ends and 3' overhangs containing less than four bases. Initiates at nicked sites in double-stranded DNA. Catalyzes the removal of nucleotides from linear or nicked double-stranded DNA in the 3' to 5' direction. Ideal for site-directed mutagenesis, preparation of single-stranded DNA for dideoxy sequencing, preparation of nested deletions in double-stranded DNA. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165571 Copy   

•   Source: Addgene

http://www.addgene.org/165524

Species: Other
Genetic Insert: T4 DNA Polymerase
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Polymerase that replicates viral genomic DNA. Possesses two enzymatic activities: DNA synthesis (as polymerase) and an exonucleolytic activity that degrades single-stranded DNA in the 3'- to 5'-direction for proofreading purposes. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165524 Copy   

•   Source: Addgene

http://www.addgene.org/165522

Species: Other
Genetic Insert: DNA Polymerase I, Large (Klenow) Fragment (3'-5' exo-)
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: DNA polymerase fragment used in fluorescent labelling for microarray, dA and dT tailing, and important in the process of ligating DNA adapters to DNA fragments. Frequently used in preparing DNA libraries for Next-Gen sequencing.The exo-Klenow polymerase is a mutant polymerase from the Klenow DNA polymerase large fragment that lacks 5'-3' polymerase activity and 3'-5' exonuclease activity. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165522 Copy   

•   Source: Addgene

http://www.addgene.org/165526

Species: Other
Genetic Insert: PstI
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Restriction enzyme that recognizes the double-stranded sequence CTGCAG and cleaves after A-5. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165526 Copy   

•   Source: Addgene

http://www.addgene.org/165529

Species: Other
Genetic Insert: DpnI
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Used to digest methylated DNA; to be expressed in dam- E. coli strains (strains which do not methylate their genomic DNA). The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165529 Copy   

•   Source: Addgene

http://www.addgene.org/165513

Species: Other
Genetic Insert: Sulfolobus DNA Polymerase IV
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Thermostable gamma-family lesion-bypass DNA Polymerase that efficiently synthesizes DNA across a variety of DNA template lesions; can be used for DNA repair. Poorly processive and error-prone. Involved in untargeted mutagenesis; copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by PolIV. Exhibits no 3'-5' exonuclease (proofreading) activity. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165513 Copy   

•   Source: Addgene

http://www.addgene.org/165516

Species: Other
Genetic Insert: SP6 RNA Polymerase
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: DNA-dependent RNA polymerase derived from SP6 bacteriophage that catalyzes the transcription of viral genes. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165516 Copy   

•   Source: Addgene

http://www.addgene.org/165517

Species: Other
Genetic Insert: Eco31IA
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Site-specific DNA methyltransferase 1; protects DNA from cleavage by Eco31I. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165517 Copy   

•   Source: Addgene

http://www.addgene.org/165514

Species: Other
Genetic Insert: 9 Degrees North DNA Ligase
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: 9 Degrees North DNA Ligase active at elevated temperatures (45-90 degrees C). Depends on the use of ATP and not NAD+ as a cofactor. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165514 Copy   

•   Source: Addgene

http://www.addgene.org/165541

Species: Other
Genetic Insert: Fpg
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Used in PreCR mix from NEB, Formamidopyrimidine DNA Glycosylase; Bifunctional DNA glycosylase with DNA N-glycosylase and AP lyase activities; N-glycosylase activity releases damaged purines, including 2,6-diamino-4-hydroxy-5-formamidopyrimidine (FapyG) and 8-oxo-7,8-dihydroguanine (8oxoG), generating an AP site. The AP lyase activity cleaves an AP site, via beta and delta-elimination, creating a 1 nucleotide DNA gap with 5' and 3' phosphate termini. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165541 Copy   

•   Source: Addgene

http://www.addgene.org/165542

Species: Other
Genetic Insert: KlenTaq1 polymerase
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Exonuclease-free, N-terminal deletion mutant of Taq DNA polymerase. Comprises substantially the same amino acid sequence as that of Thermus aquaticus DNA polymerase, excluding the N-terminal 280 amino acid residues of Thermus aquaticus DNA polymerase. Used in combination with a very low level of a thermostable DNA polymerase exhibiting a 3'-exonuclease activity (Pfu, Vent, or Deep Vent) for long PCR. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165542 Copy   

•   Source: Addgene

http://www.addgene.org/165540

Species: Other
Genetic Insert: T7EI (T7 Endonuclease I)
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: DNA Endonuclease; catalyzes the cleavage of DNA mismatches and non-beta DNA structures including Holliday junctions and cruciforms, leaving 3'-OH and 5'-phosphate. Best at C mismatches and does not recognize all DNA mismatches. To a lesser extent, cleaves across a nick in dsDNA; Key enzyme for use in genome editing mutation detection workflows. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.

Proper citation: RRID:Addgene_165540 Copy   

•   Source: Addgene