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Species: Synthetic
Genetic Insert: bArrestin2-TevC-P2A-TdTomato
Vector Backbone Description: Vector Backbone:pSyc181 P2A empty vector; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28369042
Comments: Please note that mutation R173H in TdTomato was found during Addgene's quality control. The depositor noted that this mutation does NOT affect plasmid function.
Proper citation: RRID:Addgene_89869 Copy
Species: Synthetic
Genetic Insert: 6X CArG-RE
Vector Backbone Description: Vector Backbone:pCSC-G; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27350449
Comments: CArG response element inserted upstream upstream of the ZsGreen fluorescent reporter; also expresses DsRedExpress2
Information can also be found in Moharil, et al. PLoS One. 2015 Oct 27;10(10):e0141365. doi: 10.1371 https://www.ncbi.nlm.nih.gov/pubmed/26505747 .
Proper citation: RRID:Addgene_89762 Copy
Species: Synthetic
Genetic Insert: TM-CIBN-TevN-BLITz1-TetR-VP16
Vector Backbone Description: Vector Backbone:pSyc 181 P2A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28369042
Proper citation: RRID:Addgene_89878 Copy
Species: Synthetic
Genetic Insert: bArrestin2-TevC-P2A-TdTomato
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pAAV-MCS; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28369042
Comments: Please note that mutation R173H in TdTomato was found during Addgene's quality control. The depositor noted that this mutation does NOT affect plasmid function.
Proper citation: RRID:Addgene_89873 Copy
Species: Synthetic
Genetic Insert: DRD2-V2tail-TevN-BLITz1-TetR-VP16
Vector Backbone Description: Vector Backbone:pSyc181 P2A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28369042
Proper citation: RRID:Addgene_89870 Copy
Species: Synthetic
Genetic Insert: tetO-EGFP
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pAAV-MCS; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28369042
Proper citation: RRID:Addgene_89875 Copy
Species: Synthetic
Genetic Insert: DRD2-V2tail-TevN-BLITz1-TetR-VP16
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pAAV-MCS; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28369042
Proper citation: RRID:Addgene_89874 Copy
Species: Synthetic
Genetic Insert: site-specific DNA-methyltransferase SssI
Vector Backbone Description: Backbone Size:14759; Vector Backbone:pLV hUbC-dCas9-T2A-GFP (#53191); Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28695892
Proper citation: RRID:Addgene_89793 Copy
Species: Synthetic
Genetic Insert: LSSmCherry1
Vector Backbone Description: Vector Backbone:pBAD His B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28241009
Proper citation: RRID:Addgene_89986 Copy
Species: Synthetic
Genetic Insert: LRhR
Vector Backbone Description: Backbone Marker:PMID: 3012611 with lacO1 binding site of pHG165 disabled by mutation; Vector Backbone:pHG165a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: E. coli LacI DNA binding domain, E. coli RbsR linker region, and RbsR regulatory domain . The start codon of these genes is GTG, which is an alternative MET start codon in E. coli.
Proper citation: RRID:Addgene_90054 Copy
Species: Synthetic
Genetic Insert: LLhT/V52A
Vector Backbone Description: Backbone Marker:PMID: 3012611 with lacO1 binding site of pHG165 disabled by mutation; Vector Backbone:pHG165a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22965134
Comments: E. coli LacI DNA binding domain, LacI linker region, and E. coli TreR regulatory domain . The start codon of these genes is GTG, which is an alternative MET start codon in E. coli.
Proper citation: RRID:Addgene_90050 Copy
Species: Synthetic
Genetic Insert: LGhG
Vector Backbone Description: Backbone Marker:PMID: 3012611 with lacO1 binding site of pHG165 disabled by mutation; Vector Backbone:pHG165a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19818797
Comments: E. coli LacI DNA binding domain, E. coli GalR linker region, and GalR regulatory domain . The start codon of these genes is GTG, which is an alternative MET start codon in E. coli. The GalR insert contains an E230K mutation compared to wild type.
Proper citation: RRID:Addgene_90048 Copy
Species: Synthetic
Genetic Insert: LPhP
Vector Backbone Description: Backbone Marker:PMID: 3012611; Vector Backbone:pHG165; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19818797
Comments: E. coli LacI DNA binding domain, E. coli PurR linker region, and PurR regulatory domain . The start codon of these genes is GTG, which is an alternative MET start codon in E. coli.
Proper citation: RRID:Addgene_90047 Copy
Species: Synthetic
Genetic Insert: LLhC
Vector Backbone Description: Backbone Marker:PMID: 3012611 with lacO1 binding site of pHG165 disabled by mutation; Vector Backbone:pHG165a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22965134
Comments: E. coli LacI DNA binding domain, LacI linker region, and E. coli CytR regulatory domain . The start codon of these genes is GTG, which is an alternative MET start codon in E. coli.
Proper citation: RRID:Addgene_90046 Copy
Species: Synthetic
Genetic Insert: LGhP
Vector Backbone Description: Backbone Marker:PMID: 3012611; Vector Backbone:pHG165; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24386217
Comments: E. coli LacI DNA binding domain, E. coli GalR linker region, and E. coli PurR regulatory domain . The start codon of these genes is GTG, which is an alternative MET start codon in E. coli.
Proper citation: RRID:Addgene_90065 Copy
Species: Synthetic
Genetic Insert: LLhE_AW
Vector Backbone Description: Backbone Marker:PMID: 3012611; Vector Backbone:pHG165; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22965134
Comments: E. coli LacI DNA binding domain, LacI linker region, and T. fusca CelR regulatory domain . The start codon of these genes is GTG, which is an alternative MET start codon in E. coli.
Proper citation: RRID:Addgene_90062 Copy
Species: Synthetic
Genetic Insert: LEhE
Vector Backbone Description: Backbone Marker:PMID: 3012611; Vector Backbone:pHG165; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: E. coli LacI DNA binding domain, T. fusca CelR linker region, and CelR regulatory domain . The start codon of these genes is GTG, which is an alternative MET start codon in E. coli.
Proper citation: RRID:Addgene_90061 Copy
Species: Synthetic
Genetic Insert: DEL
Vector Backbone Description: Backbone Marker:PMID: 3012611 with lacO1 binding site of pHG165 disabled by mutation; Vector Backbone:pHG165a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22965134
Comments: LLhG coding region on pHG165a interrupted with a single base-pair deletion . The start codon of these genes is GTG, which is an alternative MET start codon in E. coli.
Proper citation: RRID:Addgene_90064 Copy
Species: Synthetic
Genetic Insert: LLhP
Vector Backbone Description: Backbone Marker:PMID: 3012611; Vector Backbone:pHG165; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17436321
Comments: Second reference: PMID: 22965134. E. coli LacI DNA binding domain, LacI linker region, and E. coli PurR regulatory domain . The start codon of these genes is GTG, which is an alternative MET start codon in E. coli.
Proper citation: RRID:Addgene_90038 Copy
Species: Synthetic
Genetic Insert: Invertase followed by αMFΔ secretion tag
Vector Backbone Description: Backbone Marker:Lee et al. (2015), ACS Synth. Biol. 4, 975–986.; Backbone Size:1662; Vector Backbone:pYTK001-EntryVector; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28252957
Comments: Design of this plasmid was performed according to the described Methods in Lee et al. 2015 (A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly) and this plasmid is compatible with the existing toolkit (MoClo-YTK, Kit number 1000000061)
Proper citation: RRID:Addgene_84972 Copy
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