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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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CMV-Fast-1 H83R Resource Report Resource Website |
RRID:Addgene_16522 | FAST-1 H83R | Homo sapiens | Ampicillin | PMID:9702198 | Backbone Marker:Promega; Backbone Size:5500; Vector Backbone:pCI-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | H83R mutant | 2026-08-15 01:09:28 | 0 | |
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CMV-Fast-1 wild-type Resource Report Resource Website 1+ mentions |
RRID:Addgene_16521 | FAST-1 | Homo sapiens | Ampicillin | PMID:9702198 | To construct an hFAST-1 expression vector, normal human colon cDNA was used as the template to PCR-amplify the hFAST-1 ORF with primers NT2-exp5′ (5′-TATGCGGCCGCCACCATGGGGCCCTGCAGCG-3′) and NT2-exp3′ (5′-TATGCGGCCGCGAGCTGCTGTGTCGCAGAC-3′). The PCR product was cloned into the NotI site of pCI-neo. | Backbone Marker:Promega; Backbone Size:5500; Vector Backbone:pCI-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:28 | 3 | |
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pCI-neo beta catenin delta45 Resource Report Resource Website 1+ mentions |
RRID:Addgene_16520 | beta catenin | Homo sapiens | Ampicillin | PMID:9065402 | HCT116 has a 3-bp deletion that removes one amino acid (Ser45). | Backbone Marker:Promega; Backbone Size:5500; Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Delta 45 (HCT116 mutation) | 2026-08-15 01:09:28 | 4 |
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pLPC-mCh-ACTB-P2A-EGFP Resource Report Resource Website |
RRID:Addgene_165158 | mCherry-ACTB-P2A-EGFP | Homo sapiens | Ampicillin | PMID:33662274 | Backbone Marker:Scott Lowe; Backbone Size:5795; Vector Backbone:pLPC; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:28 | 0 | ||
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pGEX-E6AP Hect Resource Report Resource Website |
RRID:Addgene_165102 | E6AP Hect | Homo sapiens | Ampicillin | PMID:22350887 | Best yields for protein purification were found in BL21 (DE) cells using an overnight induction at 16 degrees C. | Backbone Marker:GE Healthcare; Backbone Size:4900; Vector Backbone:pGEX4T-3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | E6AP Hect domain Amino acids 495-738 (5'QLNPY-LICG3') | 2026-08-15 01:09:27 | 0 |
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pGEX4T-1-Optimized E6AP Resource Report Resource Website |
RRID:Addgene_165100 | E6AP isoform 3 | Homo sapiens | Ampicillin | PMID:23439649 | Best yields for protein purification were found in BL21 (DE) cells using an overnight induction at 16 degrees C. | Backbone Marker:GE Healthcare; Backbone Size:4900; Vector Backbone:pGEX4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | codon optimized for E. coli expression | 2026-08-15 01:09:27 | 0 |
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pGEX6P1-optimizedNedd4.2d Resource Report Resource Website |
RRID:Addgene_165103 | Nedd4.2d | Homo sapiens | Ampicillin | PMID:28972136 | Best yields for protein purification were found in BL21 (DE) cells using an overnight induction at 16 degrees C. | Backbone Marker:GE Healthcare; Backbone Size:4900; Vector Backbone:pGEX6P1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | codon optimized for E. coli expression | 2026-08-15 01:09:27 | 0 |
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pDEST15-Nedd4.2F823A Resource Report Resource Website |
RRID:Addgene_165104 | Nedd4.2d F823A | Homo sapiens | Ampicillin | PMID:30287686 | Best yields for protein purification in BL21 AI cells using an overnight induction at 16 degrees C. 5' Cloning Site: BamHI (not destroyed), 3' Cloning Site: EcoRI (not destroyed). | Backbone Marker:Thermo Fisher; Backbone Size:7013; Vector Backbone:pDEST15; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | F823A; mutation for destabilization of trimer formation | 2026-08-15 01:09:27 | 0 |
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BDS-2 3x WT (p53 binding site) Resource Report Resource Website 1+ mentions |
RRID:Addgene_16515 | p53 binding site | Homo sapiens | Ampicillin | PMID:9659898 | This reporter contains 3 copies of the wt p53-binding site from the 14-3-3 sigma promoter. The following oligonucleotide pairs were used: 5'-CCTGTAGCAT TAGCCCAGAC ATGTCCCTAC TCCGTAC-3' and 5'-GGAGTAGGGA CATGTCTGGG CTAATGCTAC AGGGTAC-3' for BDS-2. | Backbone Marker:Vogelstein Lab; Backbone Size:4900; Vector Backbone:pBV-Luc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:28 | 4 | |
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pHR TCF4 Resource Report Resource Website 1+ mentions |
RRID:Addgene_16514 | TCF4 | Homo sapiens | Kanamycin | PMID:9065401 | The TCF4 in this construct has a Q27R mutation. It is not known to affect the function. According to the depositing scientist, other mutations may be present but they have no functional consequences on standard assays for Tcf4 activity. | Backbone Size:9200; Vector Backbone:pHR; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:09:28 | 3 | |
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pcDNA/Myc TCF4 Resource Report Resource Website 1+ mentions |
RRID:Addgene_16512 | TCF4 | Homo sapiens | Ampicillin | PMID:9065401 | The TCF4 in this construct has a Q27R mutation. It is not known to affect the function. | Backbone Marker:Invitrogen; Backbone Size:0; Vector Backbone:pcDNA?; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:27 | 9 | |
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pCI-neo beta catenin S33Y Resource Report Resource Website 1+ mentions |
RRID:Addgene_16519 | beta catenin | Homo sapiens | Ampicillin | PMID:9065402 | SW48 has a C-to-A missense mutation that changes Ser33 to Tyr. Note that the NheI site is not intact and cannot be used for sub-cloning. | Backbone Marker:Promega; Backbone Size:5500; Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | S33Y (SW48 mutation) | 2026-08-15 01:09:28 | 9 |
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pBJ1 neo-hu FceRI gamma Resource Report Resource Website 1+ mentions |
RRID:Addgene_16540 | FCER1G | Homo sapiens | Ampicillin | PMID:2138619 | This construct contains only the coding sequence. It was first used in Lin S, Cicala C, Scharenberg AM, Kinet J-P (1996) Cell 85, 985-995. It can be sequenced with primers within the insert. | Backbone Marker:Mark M Davis; Backbone Size:6600; Vector Backbone:pBJ1 neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:28 | 1 | |
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pGEX4T-1-Optimized UBE1L Resource Report Resource Website |
RRID:Addgene_165099 | UbeE1L | Homo sapiens | Ampicillin | Best yields for protein purification were found in BL21 (DE) cells using an overnight induction at 16 degrees C. | Backbone Marker:GE Healthcare; Backbone Size:4900; Vector Backbone:pGEX4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | codon optimized for E. coli expression | 2026-08-15 01:09:27 | 0 | |
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CMV-ShadowY-Rhotekin(8-89)-P2A-Clover(T153M/F223R)-RhoA Resource Report Resource Website |
RRID:Addgene_165435 | ShadowY-RBD-P2A-Clover(T153M/F223R)-RhoA | Homo sapiens | Ampicillin | PMID:33531495 | Vector Backbone:Customized Vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:29 | 0 | ||
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CMV-ShadowY-CBD-P2A-Clover(T153M/F223R)-Cdc42 Resource Report Resource Website |
RRID:Addgene_165434 | ShadowY-CBD-P2A-Clover(T153M/F223R)-Cdc42 | Homo sapiens | Ampicillin | PMID:33531495 | Vector Backbone:Customized Vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:29 | 0 | ||
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pCMV3Tag8li-hHP1BP3-HA Resource Report Resource Website |
RRID:Addgene_165468 | Heterochromatin protein 1 binding protein 3 | Homo sapiens | Ampicillin | PMID:24778252 | Backbone contains new multiple cloning site and c-terminal HA epitope instead of FLAGs. Construction: XhoI-ACC-[HP1BP3 CDS 1650 bp, no stop]-Noti-a-Clai-HA epitope tag-ctt-STOP. T3 forward primer is too close for sequencing of initiation sequence. | Backbone Marker:Stratagene /Agilent; Backbone Size:5200; Vector Backbone:pCMV-3Tag-8 modified MCS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:29 | 0 | |
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TANGO1S-mScarlet-i Resource Report Resource Website 1+ mentions |
RRID:Addgene_165461 | TANGO1S | Homo sapiens | Ampicillin | PMID:34350936 | Backbone Marker:Clontech; Vector Backbone:pIRES_neo3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:29 | 1 | ||
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AAVS1-Puro-CAG-fl-STOP-fl-ZEB2-GFP-Flag-WPRE-poly(A) Resource Report Resource Website |
RRID:Addgene_165456 | ZEB2 | Homo sapiens | Ampicillin | PMID:33765444 | Backbone Size:11520; Vector Backbone:AAVS1-Puro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:29 | 0 | ||
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pCMV3Tag8li-hMBOAT7-3xFLAG Resource Report Resource Website |
RRID:Addgene_165474 | Membrane bound O-acyltransferase domain containing 7 | Homo sapiens | Ampicillin | PMID:21903422 | Backbone contains new MCS (multiple cloning site). CDS without stop codon was inserted with XhoI-NotI and it expresses in frame with 3xFLAG epitope tag. Construct insertion = Xhoi-GCC-[MBOAT7 CDS 1416 bp, no stop]-Noti-A-Clai-GTCGAG-3xFLAG-STOP | Backbone Marker:Stratagene /Agilent; Backbone Size:5200; Vector Backbone:pCMV-3Tag-8 modified MCS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:29 | 0 |
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