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  • Organism:homo sapiens (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
CMV-Fast-1 H83R
 
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RRID:Addgene_16522 FAST-1 H83R Homo sapiens Ampicillin PMID:9702198 Backbone Marker:Promega; Backbone Size:5500; Vector Backbone:pCI-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin H83R mutant 2026-08-15 01:09:28 0
CMV-Fast-1 wild-type
 
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1+ mentions
RRID:Addgene_16521 FAST-1 Homo sapiens Ampicillin PMID:9702198 To construct an hFAST-1 expression vector, normal human colon cDNA was used as the template to PCR-amplify the hFAST-1 ORF with primers NT2-exp5′ (5′-TATGCGGCCGCCACCATGGGGCCCTGCAGCG-3′) and NT2-exp3′ (5′-TATGCGGCCGCGAGCTGCTGTGTCGCAGAC-3′). The PCR product was cloned into the NotI site of pCI-neo. Backbone Marker:Promega; Backbone Size:5500; Vector Backbone:pCI-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:09:28 3
pCI-neo beta catenin delta45
 
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RRID:Addgene_16520 beta catenin Homo sapiens Ampicillin PMID:9065402 HCT116 has a 3-bp deletion that removes one amino acid (Ser45). Backbone Marker:Promega; Backbone Size:5500; Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Delta 45 (HCT116 mutation) 2026-08-15 01:09:28 4
pLPC-mCh-ACTB-P2A-EGFP
 
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RRID:Addgene_165158 mCherry-ACTB-P2A-EGFP Homo sapiens Ampicillin PMID:33662274 Backbone Marker:Scott Lowe; Backbone Size:5795; Vector Backbone:pLPC; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin 2026-08-15 01:09:28 0
pGEX-E6AP Hect
 
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RRID:Addgene_165102 E6AP Hect Homo sapiens Ampicillin PMID:22350887 Best yields for protein purification were found in BL21 (DE) cells using an overnight induction at 16 degrees C. Backbone Marker:GE Healthcare; Backbone Size:4900; Vector Backbone:pGEX4T-3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin E6AP Hect domain Amino acids 495-738 (5'QLNPY-LICG3') 2026-08-15 01:09:27 0
pGEX4T-1-Optimized E6AP
 
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RRID:Addgene_165100 E6AP isoform 3 Homo sapiens Ampicillin PMID:23439649 Best yields for protein purification were found in BL21 (DE) cells using an overnight induction at 16 degrees C. Backbone Marker:GE Healthcare; Backbone Size:4900; Vector Backbone:pGEX4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin codon optimized for E. coli expression 2026-08-15 01:09:27 0
pGEX6P1-optimizedNedd4.2d
 
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RRID:Addgene_165103 Nedd4.2d Homo sapiens Ampicillin PMID:28972136 Best yields for protein purification were found in BL21 (DE) cells using an overnight induction at 16 degrees C. Backbone Marker:GE Healthcare; Backbone Size:4900; Vector Backbone:pGEX6P1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin codon optimized for E. coli expression 2026-08-15 01:09:27 0
pDEST15-Nedd4.2F823A
 
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RRID:Addgene_165104 Nedd4.2d F823A Homo sapiens Ampicillin PMID:30287686 Best yields for protein purification in BL21 AI cells using an overnight induction at 16 degrees C. 5' Cloning Site: BamHI (not destroyed), 3' Cloning Site: EcoRI (not destroyed). Backbone Marker:Thermo Fisher; Backbone Size:7013; Vector Backbone:pDEST15; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin F823A; mutation for destabilization of trimer formation 2026-08-15 01:09:27 0
BDS-2 3x WT (p53 binding site)
 
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1+ mentions
RRID:Addgene_16515 p53 binding site Homo sapiens Ampicillin PMID:9659898 This reporter contains 3 copies of the wt p53-binding site from the 14-3-3 sigma promoter. The following oligonucleotide pairs were used: 5'-CCTGTAGCAT TAGCCCAGAC ATGTCCCTAC TCCGTAC-3' and 5'-GGAGTAGGGA CATGTCTGGG CTAATGCTAC AGGGTAC-3' for BDS-2. Backbone Marker:Vogelstein Lab; Backbone Size:4900; Vector Backbone:pBV-Luc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:09:28 4
pHR TCF4
 
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1+ mentions
RRID:Addgene_16514 TCF4 Homo sapiens Kanamycin PMID:9065401 The TCF4 in this construct has a Q27R mutation. It is not known to affect the function. According to the depositing scientist, other mutations may be present but they have no functional consequences on standard assays for Tcf4 activity. Backbone Size:9200; Vector Backbone:pHR; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:09:28 3
pcDNA/Myc TCF4
 
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1+ mentions
RRID:Addgene_16512 TCF4 Homo sapiens Ampicillin PMID:9065401 The TCF4 in this construct has a Q27R mutation. It is not known to affect the function. Backbone Marker:Invitrogen; Backbone Size:0; Vector Backbone:pcDNA?; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:09:27 9
pCI-neo beta catenin S33Y
 
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1+ mentions
RRID:Addgene_16519 beta catenin Homo sapiens Ampicillin PMID:9065402 SW48 has a C-to-A missense mutation that changes Ser33 to Tyr. Note that the NheI site is not intact and cannot be used for sub-cloning. Backbone Marker:Promega; Backbone Size:5500; Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin S33Y (SW48 mutation) 2026-08-15 01:09:28 9
pBJ1 neo-hu FceRI gamma
 
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1+ mentions
RRID:Addgene_16540 FCER1G Homo sapiens Ampicillin PMID:2138619 This construct contains only the coding sequence. It was first used in Lin S, Cicala C, Scharenberg AM, Kinet J-P (1996) Cell 85, 985-995. It can be sequenced with primers within the insert. Backbone Marker:Mark M Davis; Backbone Size:6600; Vector Backbone:pBJ1 neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:09:28 1
pGEX4T-1-Optimized UBE1L
 
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RRID:Addgene_165099 UbeE1L Homo sapiens Ampicillin Best yields for protein purification were found in BL21 (DE) cells using an overnight induction at 16 degrees C. Backbone Marker:GE Healthcare; Backbone Size:4900; Vector Backbone:pGEX4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin codon optimized for E. coli expression 2026-08-15 01:09:27 0
CMV-ShadowY-Rhotekin(8-89)-P2A-Clover(T153M/F223R)-RhoA
 
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RRID:Addgene_165435 ShadowY-RBD-P2A-Clover(T153M/F223R)-RhoA Homo sapiens Ampicillin PMID:33531495 Vector Backbone:Customized Vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:09:29 0
CMV-ShadowY-CBD-P2A-Clover(T153M/F223R)-Cdc42
 
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RRID:Addgene_165434 ShadowY-CBD-P2A-Clover(T153M/F223R)-Cdc42 Homo sapiens Ampicillin PMID:33531495 Vector Backbone:Customized Vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:09:29 0
pCMV3Tag8li-hHP1BP3-HA
 
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RRID:Addgene_165468 Heterochromatin protein 1 binding protein 3 Homo sapiens Ampicillin PMID:24778252 Backbone contains new multiple cloning site and c-terminal HA epitope instead of FLAGs. Construction: XhoI-ACC-[HP1BP3 CDS 1650 bp, no stop]-Noti-a-Clai-HA epitope tag-ctt-STOP. T3 forward primer is too close for sequencing of initiation sequence. Backbone Marker:Stratagene /Agilent; Backbone Size:5200; Vector Backbone:pCMV-3Tag-8 modified MCS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:09:29 0
TANGO1S-mScarlet-i
 
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1+ mentions
RRID:Addgene_165461 TANGO1S Homo sapiens Ampicillin PMID:34350936 Backbone Marker:Clontech; Vector Backbone:pIRES_neo3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:09:29 1
AAVS1-Puro-CAG-fl-STOP-fl-ZEB2-GFP-Flag-WPRE-poly(A)
 
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RRID:Addgene_165456 ZEB2 Homo sapiens Ampicillin PMID:33765444 Backbone Size:11520; Vector Backbone:AAVS1-Puro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:09:29 0
pCMV3Tag8li-hMBOAT7-3xFLAG
 
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RRID:Addgene_165474 Membrane bound O-acyltransferase domain containing 7 Homo sapiens Ampicillin PMID:21903422 Backbone contains new MCS (multiple cloning site). CDS without stop codon was inserted with XhoI-NotI and it expresses in frame with 3xFLAG epitope tag. Construct insertion = Xhoi-GCC-[MBOAT7 CDS 1416 bp, no stop]-Noti-A-Clai-GTCGAG-3xFLAG-STOP Backbone Marker:Stratagene /Agilent; Backbone Size:5200; Vector Backbone:pCMV-3Tag-8 modified MCS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:09:29 0

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