Searching the RRID Resource Information Network
Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
Comments |
||||
|---|---|---|---|---|---|---|---|---|---|---|
|
pOpen-T4gene30 Resource Report Resource Website |
RRID:Addgene_165554 | T4-DNA Ligase | Other | Ampicillin | Ligase derived from T4 bacteriophage expressed in the early stage of lytic development. Has been implicated in T4 DNA synthesis and genetic recombination. May also play a role in T4 DNA repair. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:30 | 0 | ||
|
pOpen-ALPI Resource Report Resource Website |
RRID:Addgene_165555 | CIP (calf intestinal phosphatase) | Other | Ampicillin | CIAP reporter; can be used in spectrophotometric or luminescence assay when appropriate substrate is used. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:30 | 0 | ||
|
pOpen-T5gene12/13 Resource Report Resource Website |
RRID:Addgene_165559 | T5 Exonuclease | Other | Ampicillin | Double-stranded DNA specific exonuclease and single-stranded DNA endonuclease. Initiates at the 5' termini of linear or nicked double-stranded DNA. Cleaves linear or nicked double-stranded DNA in the 5' to 3' direction. Ideal for removal of incomplete ligation products from ligated circular double-stranded DNA, degradation of denatured DNA from alkaline-based plasmid purification methods for improved DNA cloning, and degradation of contaminating linear and nicked DNA in plasmid samples. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:30 | 0 | ||
|
pOpen-BovDNTT Resource Report Resource Website |
RRID:Addgene_165580 | Bovine DNTT | Other | Ampicillin | Adds dT at the end of PCR blunted DNA fragments/vectors that can then be used in TOPO cloning; Addition of homopolymer tails to the 3' ends of DNA; Labeling the 3' ends of DNA with modified nucleotides (e.g., ddNTP, DIG-dUTP); TUNEL assay (in situ localization of apoptosis); TdT dependent PCR. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:30 | 0 | ||
|
pOpen-HSlig3 Resource Report Resource Website |
RRID:Addgene_165574 | DNA Ligase III | Other | Ampicillin | Repairs single strand breaks in DNA efficiently. Unable to perform either blunt-end joining or AMP- dependent relaxation of supercoiled DNA (Elder, R.H. et al . , Bur. J. Biochem.. 203:53-58 (1992)). The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:30 | 0 | ||
|
pOpen-phi29pol Resource Report Resource Website |
RRID:Addgene_165573 | phi29 DNA Polymerase | Other | Ampicillin | DNA polymerase responsible for protein-primed viral DNA replication by strand displacement with high processivity and fidelity. Possesses three enzymatic activities: DNA synthesis (polymerase), primer terminal protein (TP) deoxynucleotidylation, and 3' to 5' exonuclease activity. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:30 | 0 | ||
|
pOpen-NcoIM Resource Report Resource Website |
RRID:Addgene_165578 | M.NcoI | Other | Ampicillin | Site-specific DNA methyltransferase; protects DNA from cleavage by NcoI. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:30 | 0 | ||
|
pOpen-P45 Resource Report Resource Website |
RRID:Addgene_165570 | P45 | Other | Ampicillin | Significantly improves maximum amplicon size and PCR yields for Family B polymerases like Pfu or Phusion. Comparable to ArchaeMaxx/PfuTurbo. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:30 | 0 | ||
|
pOpen-Exonuclease III E. coli Resource Report Resource Website |
RRID:Addgene_165571 | Exonuclease III E. coli | Other | Ampicillin | Double-stranded DNA specific exonuclease. Initiates at the 3' termini of linear double-stranded DNA with 5' overhangs or blunt ends and 3' overhangs containing less than four bases. Initiates at nicked sites in double-stranded DNA. Catalyzes the removal of nucleotides from linear or nicked double-stranded DNA in the 3' to 5' direction. Ideal for site-directed mutagenesis, preparation of single-stranded DNA for dideoxy sequencing, preparation of nested deletions in double-stranded DNA. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:30 | 0 | ||
|
pOpen-T4gene43 Resource Report Resource Website |
RRID:Addgene_165524 | T4 DNA Polymerase | Other | Ampicillin | Polymerase that replicates viral genomic DNA. Possesses two enzymatic activities: DNA synthesis (as polymerase) and an exonucleolytic activity that degrades single-stranded DNA in the 3'- to 5'-direction for proofreading purposes. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:30 | 0 | ||
|
pOpen-K12polLF (exo-) Resource Report Resource Website |
RRID:Addgene_165522 | DNA Polymerase I, Large (Klenow) Fragment (3'-5' exo-) | Other | Ampicillin | DNA polymerase fragment used in fluorescent labelling for microarray, dA and dT tailing, and important in the process of ligating DNA adapters to DNA fragments. Frequently used in preparing DNA libraries for Next-Gen sequencing.The exo-Klenow polymerase is a mutant polymerase from the Klenow DNA polymerase large fragment that lacks 5'-3' polymerase activity and 3'-5' exonuclease activity. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:30 | 0 | ||
|
pOpen-PstIR Resource Report Resource Website |
RRID:Addgene_165526 | PstI | Other | Ampicillin | Restriction enzyme that recognizes the double-stranded sequence CTGCAG and cleaves after A-5. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:30 | 0 | ||
|
pOpen-DpnIR Resource Report Resource Website |
RRID:Addgene_165529 | DpnI | Other | Ampicillin | Used to digest methylated DNA; to be expressed in dam- E. coli strains (strains which do not methylate their genomic DNA). The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:30 | 0 | ||
|
pOpen-dbh Resource Report Resource Website |
RRID:Addgene_165513 | Sulfolobus DNA Polymerase IV | Other | Ampicillin | Thermostable gamma-family lesion-bypass DNA Polymerase that efficiently synthesizes DNA across a variety of DNA template lesions; can be used for DNA repair. Poorly processive and error-prone. Involved in untargeted mutagenesis; copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by PolIV. Exhibits no 3'-5' exonuclease (proofreading) activity. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:29 | 0 | ||
|
pOpen-SP6rpol Resource Report Resource Website |
RRID:Addgene_165516 | SP6 RNA Polymerase | Other | Ampicillin | DNA-dependent RNA polymerase derived from SP6 bacteriophage that catalyzes the transcription of viral genes. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:29 | 0 | ||
|
pOpen-Eco31IA Resource Report Resource Website |
RRID:Addgene_165517 | Eco31IA | Other | Ampicillin | Site-specific DNA methyltransferase 1; protects DNA from cleavage by Eco31I. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:30 | 0 | ||
|
pOpen-9N7lig Resource Report Resource Website |
RRID:Addgene_165514 | 9 Degrees North DNA Ligase | Other | Ampicillin | 9 Degrees North DNA Ligase active at elevated temperatures (45-90 degrees C). Depends on the use of ATP and not NAD+ as a cofactor. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:29 | 0 | ||
|
pOpen-Fpg Resource Report Resource Website |
RRID:Addgene_165541 | Fpg | Other | Ampicillin | Used in PreCR mix from NEB, Formamidopyrimidine DNA Glycosylase; Bifunctional DNA glycosylase with DNA N-glycosylase and AP lyase activities; N-glycosylase activity releases damaged purines, including 2,6-diamino-4-hydroxy-5-formamidopyrimidine (FapyG) and 8-oxo-7,8-dihydroguanine (8oxoG), generating an AP site. The AP lyase activity cleaves an AP site, via beta and delta-elimination, creating a 1 nucleotide DNA gap with 5' and 3' phosphate termini. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:30 | 0 | ||
|
pOpen-KTHEAQpol Resource Report Resource Website |
RRID:Addgene_165542 | KlenTaq1 polymerase | Other | Ampicillin | Exonuclease-free, N-terminal deletion mutant of Taq DNA polymerase. Comprises substantially the same amino acid sequence as that of Thermus aquaticus DNA polymerase, excluding the N-terminal 280 amino acid residues of Thermus aquaticus DNA polymerase. Used in combination with a very low level of a thermostable DNA polymerase exhibiting a 3'-exonuclease activity (Pfu, Vent, or Deep Vent) for long PCR. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:30 | 0 | ||
|
pOpen-T7EI Resource Report Resource Website |
RRID:Addgene_165540 | T7EI (T7 Endonuclease I) | Other | Ampicillin | DNA Endonuclease; catalyzes the cleavage of DNA mismatches and non-beta DNA structures including Holliday junctions and cruciforms, leaving 3'-OH and 5'-phosphate. Best at C mismatches and does not recognize all DNA mismatches. To a lesser extent, cleaves across a nick in dsDNA; Key enzyme for use in genome editing mutation detection workflows. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/. | Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:30 | 0 |
Can't find your Plasmid?
We recommend that you click next to the search bar to check some helpful tips on searches and refine your search firstly. If you want to find a specific plasmid, it's easier to enter an RRID or an Addgene Catalog Number to search. You can refine the search results using Facets on the left side of the search results page. If you are on the table view, you can also search in a specific column by clicking the column title and enter the keywords.
If you still could not find your plasmid in the search results, please help us by registering it into the system — it's easy. Register it with Addgene.
Welcome to the RRID Resources search. From here you can search through a compilation of resources used by RRID and see how data is organized within our community.
You are currently on the Community Resources tab looking through categories and sources that RRID has compiled. You can navigate through those categories from here or change to a different tab to execute your search through. Each tab gives a different perspective on data.
If you have an account on RRID then you can log in from here to get additional features in RRID such as Collections, Saved Searches, and managing Resources.
Here is the search term that is being executed, you can type in anything you want to search for. Some tips to help searching:
If you are logged into RRID you can add data records to your collections to create custom spreadsheets across multiple sources of data.
Here are the facets that you can filter the data by.
If you have any further questions please check out our FAQs Page to ask questions and see our tutorials. Click this button to view this tutorial again.
