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Species: Other
Genetic Insert: Bst DNA Polymerase, Full Length
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Full length DNA polymerase from Bacillus stearothermophilus. Has 5'-3' polymerase and double-strand specific 5'-3' exonuclease activity, but lacks the 3'-5' exonuclease activity. Used for isothermal DNA amplification, primer extension, etc.
The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.
Proper citation: RRID:Addgene_165543 Copy
Species: Other
Genetic Insert: T5 DNA Polymerase
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Replicates viral genomic DNA. Possesses two enzymatic activities: DNA synthesis (polymerase) and an exonucleolytic activity that degrades single-stranded DNA in the 3'-5' direction for proofreading purposes. The DNA synthesis very likely occurs by strand displacement.
The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.
Proper citation: RRID:Addgene_165544 Copy
Species: Other
Genetic Insert: M1.SapI
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Site-specific DNA methyltransferase 1; protects DNA from cleavage by SapI.
The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.
Proper citation: RRID:Addgene_165547 Copy
Species: Other
Genetic Insert: T4-BGT
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: T4 Phage beta-glucosyltransferase specifically transfers the glucose moiety of uridine diphosphoglucose (UDP-Glc) to the 5-hydroxymethylcytosine (5-hmC) residues in double-stranded DNA, making beta-glucosyl-5-hydroxymethylcytosine. Used in methylation analysis.
The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.
Proper citation: RRID:Addgene_165530 Copy
Species: Other
Genetic Insert: Taq DNA Ligase
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Thermostable ligase for incorporation of phosphorylated oligonucleotides during PCR and Ligase Chain Reaction.
The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.
Proper citation: RRID:Addgene_165535 Copy
Species: Other
Genetic Insert: E. coli DNA Polymerase I
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: DNA polymerase that (in addition to polymerase activity) exhibits 3'-5' and 5'-3' exonuclease activity. Able to utilize nicked circular duplex DNA as a template and can unwind the parental DNA strand from its template.
The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.
Proper citation: RRID:Addgene_165532 Copy
Species: Other
Genetic Insert: Tth DNA polymerase
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: DNA Polymerase whose activity is resistant to prolonged incubations at high temperatures (95 degrees C); can therefore be used for PCR amplification of DNA fragments. Also accepts modified deoxyribonucleotides as substrates and can thus be used to label DNA fragments with radiolabeled nucleotides, digoxigenin, or biotin. Intrinsic Mn-dependent RT activity allows the enzyme to efficiently transcribe RNA targets into cDNA. Tth DNA Polymerase has been used for real time PCR.
The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.
Proper citation: RRID:Addgene_165533 Copy
Species: Other
Genetic Insert: Bst HF
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Bst-HF is a high-fidelity strand displacing polymerase that is able to overcome complex/problematic sequences, allowing amplification of large fragments and DNA sequences with over 80% GC content. Bst-HF has an error rate of about 7.8x10^-7, a fidelity about 20 times higher than standard Bst, and better than Phusion High-Fidelity DNA Polymerase in High-GC buffer.
The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.
Proper citation: RRID:Addgene_165539 Copy
Species: Other
Genetic Insert: M.PstI
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Methylase that recognizes the double-stranded sequence CTGCAG, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the PstI endonuclease.
The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.
Proper citation: RRID:Addgene_165536 Copy
Species: Other
Genetic Insert: SapI
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Type II restriction enzyme that recognizes the double-stranded sequence GCTCTTC(N) and cleaves after N-1.
The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.
Proper citation: RRID:Addgene_165537 Copy
Species: Other
Genetic Insert: HCoV-HKU1 S (spike)
Vector Backbone Description: Vector Backbone:pDest-303; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32504802
Comments: DNA sequence optimized for mammalian expression
Proper citation: RRID:Addgene_166014 Copy
Species: Other
Genetic Insert: HCoV-NL63 S (spike)
Vector Backbone Description: Vector Backbone:pDest-303; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32504802
Comments: DNA sequence optimized for mammalian expression
Proper citation: RRID:Addgene_166017 Copy
Species: Other
Genetic Insert: SARS-CoV-2 S (spike)
Vector Backbone Description: Vector Backbone:pDest-303; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32504802
Comments: DNA sequence optimized for mammalian expression
Proper citation: RRID:Addgene_166011 Copy
Species: Other
Genetic Insert: GPI anchored TurboID
Vector Backbone Description: Vector Backbone:pZac2.1; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33177716
Proper citation: RRID:Addgene_166055 Copy
Species: Other
Genetic Insert: mEGFP
Vector Backbone Description: Vector Backbone:pet22b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33606996
Proper citation: RRID:Addgene_166439 Copy
Species: Other
Genetic Insert: Spaghetti monster Myc
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_166239 Copy
Species: Other
Genetic Insert: Spaghetti monster HA
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_166238 Copy
Species: Other
Genetic Insert: mCardinal
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_166236 Copy
Species: Other
Genetic Insert: I-sceI, sacB
Vector Backbone Description: Vector Backbone:R6K; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32448155
Comments: Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_166651 Copy
Species: Other
Genetic Insert: ccdB
Vector Backbone Description: Vector Backbone:pTargetF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33764372
Proper citation: RRID:Addgene_166581 Copy
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