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Species: Danio rerio
Genetic Insert: wnt-1
Vector Backbone Description: Backbone Marker:Doug Melton Lab (Addgene #15030); Backbone Size:3000; Vector Backbone:SP64T; Vector Types:; Bacterial Resistance:Ampicillin
Comments: Insert: wnt-1 myc.
Insert size: 1.2 Kb.
Transcription: linearize with Pst1, transcribe with Sp6.
Plasmid History:
Constructed by Greg Kelly on date of 1/27/93, page 20 Book 2.
Notes for Use:
There is also a construct where wnt-1 myc is clone in antisense orientation.
Proper citation: RRID:Addgene_17042 Copy
Species: Danio rerio
Genetic Insert: wnt-4 cDNA
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pT7TS; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8541205
Comments: Insert: wnt-4 (zebrafish) cDNA.
Insert size: 1.3 Kb.
To make an in situ probe, linearize with EcoRV and transcribe with SP6.
To make sense RNA, linearize with Xba1 and transcribe with T7.
Plasmid History:
Constructed by Anne Ungar on date of 6/8/93.
Species of Sequence Origin: Zebrafish.
Notes for Use:
Note presence of R1, Sma, BamH1 sites brought in from pBSSK during construction. The 1274 nucleotide Nsi1 fragment containing the complete wnt-4 coding region was ligated into the Pst1 site of pBSSK (zf4 in pBSSK clone 5). The 5' end is near the Kpn1 site. A 1.3 Kb fragment was cut out with EcoRV and Spe1, isolated and ligated into EcoRV/Spe1 cut pT7Ts.
Citation: Ungar et al., Mechanisms of Development 52 (1995)
Proper citation: RRID:Addgene_17043 Copy
Species: Synthetic
Genetic Insert: H2B-RFP
Vector Backbone Description: Vector Backbone:pLV; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33644903
Proper citation: RRID:Addgene_170363 Copy
Species: Homo sapiens
Genetic Insert: Cas9-T2A-GFP
Vector Backbone Description: Vector Backbone:pLV; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31320637
Proper citation: RRID:Addgene_170361 Copy
Species: Homo sapiens
Genetic Insert: Cas9-T2A-GFP
Vector Backbone Description: Vector Backbone:pLV; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31320637
Proper citation: RRID:Addgene_170362 Copy
Species: Synthetic
Genetic Insert: flex.hM3Dq.Cherry
Vector Backbone Description: Backbone Size:6979; Vector Backbone:pLV; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33476547
Proper citation: RRID:Addgene_170360 Copy
Species: Drosophila melanogaster
Genetic Insert: ssrp1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7440; Vector Backbone:pET-DEST42; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31242411
Proper citation: RRID:Addgene_170439 Copy
Species: Homo sapiens
Genetic Insert: solute carrier family 7 member 11
Vector Backbone Description: Backbone Size:8687; Vector Backbone:plenti6.3-V5; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28630042
Proper citation: RRID:Addgene_170427 Copy
Species: SARS-CoV-2
Genetic Insert: SARS-CoV-2 Spike
Vector Backbone Description: Backbone Size:4971; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33969320
Proper citation: RRID:Addgene_170389 Copy
Species: Homo sapiens
Genetic Insert: mTDel_EPACdDEPCD_cp173Ven(ST)_Ven(ST)
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21559477
Proper citation: RRID:Addgene_170338 Copy
Species: Synthetic
Genetic Insert: hU6-BB-LacZ and EF1A-EGFP-2A-Puro
Vector Backbone Description: Backbone Size:7466; Vector Backbone:3rd Generation lentiviral vector; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34050182
Proper citation: RRID:Addgene_170459 Copy
Species: Homo sapiens
Genetic Insert: CFP(ST)_EPACdDEP _Y(UST)
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15550931
Proper citation: RRID:Addgene_170336 Copy
Species: Rattus norvegicus
Genetic Insert: NR1
Vector Backbone Description: Backbone Size:9370; Vector Backbone:pFUGW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33514725
Proper citation: RRID:Addgene_170457 Copy
Species: Mus musculus
Genetic Insert: GluA2
Vector Backbone Description: Backbone Size:9300; Vector Backbone:pFUGW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33514725
Proper citation: RRID:Addgene_170455 Copy
Species: Homo sapiens
Genetic Insert: CFP(ST)_EPAC full length_Y(UST)
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15550931
Proper citation: RRID:Addgene_170335 Copy
Species: Mus musculus
Genetic Insert: GluA2
Vector Backbone Description: Backbone Size:9370; Vector Backbone:pFUGW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33514725
Proper citation: RRID:Addgene_170454 Copy
Species: Homo sapiens
Genetic Insert: RORG isoform 1
Vector Backbone Description: Backbone Marker:LifeSensor; Backbone Size:5476; Vector Backbone:pESUMO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34547329
Proper citation: RRID:Addgene_170330 Copy
Species: Homo sapiens
Genetic Insert: RPL22
Vector Backbone Description: Vector Backbone:pLV; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32516938
Proper citation: RRID:Addgene_170327 Copy
Species: MERS
Genetic Insert: MERS spike D12
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pcDNA3.3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32540903
Proper citation: RRID:Addgene_170448 Copy
Species: SARS-CoV-2, 501V2 (B.1.351)
Genetic Insert: SARS-CoV-2 501V2 spike D18
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pcDNA3.3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34016740
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.02.16.430500v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_170449 Copy
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