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  • Organism:homo sapiens (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pCMV3Tag8li-hADARB2-3xFLAG
 
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RRID:Addgene_165470 Adenosine deaminase RNA specific B2 (inactive) Homo sapiens Ampicillin PMID:24778252 Backbone contains new MCS (multiple cloning site). MCS is changed to: ctcgagctgaagcttcatggatccaaagcggccgcaatcgat (XhoI-ctg-HindIII-cat-BamHI-aaa-NotI-a-ClaI). CDS without stop codon was inserted into MCS at unkown restriction enzyme sites, and it expresses in frame with 3xFLAG epitope tag. Xhoi was probably the 5' cloning site. Backbone Marker:Stratagene /Agilent; Backbone Size:5200; Vector Backbone:pCMV-3Tag-8 modified MCS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:09:29 0
pGST-PPARgamma
 
Resource Report
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1+ mentions
RRID:Addgene_16549 PPAR gamma Homo sapiens Ampicillin PMID:10555149 Note that there are some discrepancies between the insert in this plasmid and the canonical GenBank PPARgamma. The Vogelstein lab maintains that this plasmid worked as specified when they made and used it. Backbone Marker:Amersham; Backbone Size:5000; Vector Backbone:pGEX-2TK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin N-terminal DNA-binding domain: aa 1-248 2026-08-15 01:09:29 2
pSico_U6-PGC1a1 sgRNA
 
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RRID:Addgene_165425 gRNA against PGC-1a variant 1 Homo sapiens Ampicillin PMID:33626346 Backbone Size:9580; Vector Backbone:pSico; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:09:28 0
pBI-p73 wt/EGFP
 
Resource Report
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1+ mentions
RRID:Addgene_16545 p73 Homo sapiens Ampicillin PMID:10588737 Tet-responsive p73 expression construct. Backbone Size:5210; Vector Backbone:pBI-MCS-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:09:29 1
pSico_U6-Pan-PGC1a sgRNA
 
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RRID:Addgene_165426 gRNA against human Total PGC-1a variants Homo sapiens Ampicillin PMID:33626346 Backbone Size:9580; Vector Backbone:pSico; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:09:28 0
pGST-PPARalpha
 
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RRID:Addgene_16550 PPAR alpha Homo sapiens Ampicillin PMID:10555149 Backbone Marker:Amersham; Backbone Size:5000; Vector Backbone:pGEX-2TK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin N-terminal DNA-binding domain: aa 1-249 2026-08-15 01:09:29 0
pSIN-TRE-H2BeGFP-rtTA2
 
Resource Report
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1+ mentions
RRID:Addgene_165494 Human histone H2B Homo sapiens Ampicillin PMID:29944140 ABOUT BACKBONE: The lentiviral backbone was originally designed and constructed by Barde I et al. (Barde I et al. Mol Ther. 2006. 13(2): 382-90. PMID: 16275162). ABOUT TEST DIGESTION: Since Xba I restriction site is lost during cloning, we recommend checking the plasmid using the enzymes: Xho I and Nhe I. You should get two fragments around 1600 bp (insert+vector) and 7500 bp (vector). Backbone Marker:-; Backbone Size:8023; Vector Backbone:pRRL-cPPT-hPGKTMPrtTA-WPRE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin - 2026-08-15 01:09:29 1
dCas9-MSK1
 
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RRID:Addgene_165601 ribosomal protein S6 kinase A5 Homo sapiens Ampicillin PMID:33563994 Vector Backbone:LentiCRISPR v2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:09:31 0
pIVEX2.3 TEM-5
 
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RRID:Addgene_16561 TEM5 Homo sapiens Ampicillin PMID:11559528 Backbone Marker:Boehringer; Backbone Size:3553; Vector Backbone:pIVEX2.3-MCS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin Extracellular region 2026-08-15 01:09:31 0
pGEX TEM-1
 
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RRID:Addgene_16560 TEM1 Homo sapiens Ampicillin PMID:11559528 Backbone Marker:Amersham; Backbone Size:5000; Vector Backbone:pGEX-2TK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin Extracellular region 2026-08-15 01:09:30 0
pGL3-OF (mutant TCF-4 sites)
 
Resource Report
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10+ mentions
RRID:Addgene_16559 TCF-4 binding sites Homo sapiens Ampicillin PMID:10749138 TCF-4 mutant binding sequence - CCTTTGGC Backbone Marker:Promega; Backbone Size:4800; Vector Backbone:pGL3-Basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin 3 copies of mutant Tcf-4 binding sites 2026-08-15 01:09:30 11
MadMyc-HA
 
Resource Report
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1+ mentions
RRID:Addgene_16557 MADMYC Homo sapiens Ampicillin PMID:10688915 MADMYC: the amino terminal transactivation domain of c-Myc (aa 1-263) was removed and replaced with the amino terminal transcriptional repression domain (aa 1-38, the Sin3-interaction region) of Mad. Backbone Size:0; Vector Backbone:n/a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Dominant negative mutant 2026-08-15 01:09:30 2
CMV-CDX2-MUT
 
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RRID:Addgene_16554 CDX2 Homo sapiens Kanamycin PMID:10490837 RKO (colorectal cancer cell line) mutation: 4 base-pair deletion (AGGCAGG to AGG) within the sequence corresponding to helix 3 of the CDX2 homeodomain. Backbone Marker:Vogelstein Lab; Backbone Size:7462; Vector Backbone:pHRCMV; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin RKO mutation 2026-08-15 01:09:30 0
CMV-CDX2-WT
 
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RRID:Addgene_16553 CDX2 Homo sapiens Kanamycin PMID:10490837 Backbone Marker:Vogelstein Lab; Backbone Size:7462; Vector Backbone:pHRCMV; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin 2026-08-15 01:09:30 0
pHRCMV 14-3-3 sigma
 
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RRID:Addgene_16552 14-3-3 sigma Homo sapiens Kanamycin PMID:9659898 Backbone Marker:Vogelstein Lab; Backbone Size:7462; Vector Backbone:pHRCMV; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin 2026-08-15 01:09:29 0
pFredB-PTTG1
 
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RRID:Addgene_16585 PTTG1 Homo sapiens Ampicillin PMID:11371342 5 kb 3' targeting element, should be linearized with SalI prior to transfection. Backbone Size:3939; Vector Backbone:pFredB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 3' targeting element 2026-08-15 01:09:32 0
pBV-Luc mut MBS1
 
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RRID:Addgene_16566 c-MYC binding sites Homo sapiens Ampicillin PMID:10688915 Different combinations of oligonucleotide pairs (A+B, A+D, C+B, C+D) were annealed and converted to double-stranded fragments through one PCR cycle. These promoter fragments were subcloned into pBV-luc. Further polymerase-derived mutants were identified while sequencing the reporter constructs. Backbone Marker:Vogelstein Lab; Backbone Size:4867; Vector Backbone:pBV-Luc; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin Mutant MBS1 2026-08-15 01:09:31 0
pBV-Luc mut MBS1-4
 
Resource Report
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1+ mentions
RRID:Addgene_16565 c-MYC binding sites Homo sapiens Ampicillin PMID:10688915 To generate reporter constructs, the following oligonucleotides were used: 5'-CCGGTACCGG GTTGTGGCAG CCAGTCACCT GCCCGCCGCG TAGCCACACC TCTGCTCCTC AGAGCAATGT CAAGCGGTCA CCTGTGATAG CAACAGATCA CCTGGCTGCC ATCGCCCCTC-3' [Oligo C , for mutant MBS1-3]; 5'-ATGAATTCCG GACGTTCTGG GCAGGTGACC GCCACCCATG CGCTGAGGGG CGGACAGGAG GTGCTTCGAC TGGGAGGAGG GCGAAGAGTG TAAGGGGGCG GAGGGGCGAT GGCAGCCAGG-3' [Oligo D, for mutant MBS4]. Different combinations of oligonucleotide pairs (A+B, A+D, C+B, C+D) were annealed and converted to double-stranded fragments through one PCR cycle. These promoter fragments were subcloned into pBV-luc. Further polymerase-derived mutants were identified while sequencing the reporter constructs. Backbone Marker:Vogelstein Lab; Backbone Size:4867; Vector Backbone:pBV-Luc; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin Mutant MBS1-4 2026-08-15 01:09:31 4
pBV-Luc wt MBS1-4
 
Resource Report
Resource Website
10+ mentions
RRID:Addgene_16564 c-MYC binding sites Homo sapiens Ampicillin PMID:10688915 To generate reporter constructs, the following oligonucleotides were used: 5'-CCGGTACCGG GTTGTGGCAG CCAGTCACGT GCCCGCCGCG TAGCCACACC TCTGCTCCTC AGAGCAATGT CAAGCGGTCA CGTGTGATAG CAACAGATCA CGTGGCTGCC ATCGCCCCTC-3' [Oligo A, for wild-type c-MYC binding sites (MBS)1-3]; 5'-ATGAATTCCG GACGTTCTGG GCACGTGACC GCCACCCATG CGCTGAGGGG CGGACAGGAG GTGCTTCGAC TGGGAGGAGG GCGAAGAGTG TAAGGGGGCG GAGGGGCGAT GGCAGCC-3' [Oligo B, for wild-type MBS4]. Different combinations of oligonucleotide pairs (A+B, A+D, C+B, C+D) were annealed and converted to double-stranded fragments through one PCR cycle. These promoter fragments were subcloned into pBV-luc. Further polymerase-derived mutants were identified while sequencing the reporter constructs. Backbone Marker:Vogelstein Lab; Backbone Size:4867; Vector Backbone:pBV-Luc; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin 2026-08-15 01:09:31 13
Ad-CBR (GFP/cAPC)
 
Resource Report
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RRID:Addgene_16576 cAPC Homo sapiens Kanamycin PMID:10749138 The cAPC containing amino acids 958-2075 (nucleotides 2890–6240) was isolated from pCMV-APC by BglII digestion. This fragment was subcloned into the pEGFP-C1 (Clontech). The cassette containing the EGFP-tagged cAPC was further subcloned into the shuttle vector (pShuttle) using Apal I and Mlu I sites. Backbone Marker:Vogelstein Lab; Backbone Size:0; Vector Backbone:pShuttle / pAdTrack ?; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin Residues 958-2076 2026-08-15 01:09:31 0

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