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Species: Homo sapiens
Genetic Insert: BAX knockout
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript II SK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11062132
Comments: This targeting construct consists of two homologous arms and a hygromycin-resistant gene.
Proper citation: RRID:Addgene_16575 Copy
Species: Homo sapiens
Genetic Insert: CDK4
Vector Backbone Description: Backbone Size:0; Vector Backbone:G1BgSVNA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10688915
Comments: The CDK4 ORF was generated by PCR using the expressed sequence tag W77860 as a template and the primers 5'-GCGGATCCGC GGCCGCCTTC CACCATGGCT ACCTCTCGAT CTGAGC-3' and 5'-CGGTCGACTC ACTCCGGATT ACCTTCATC-3'. The resulting product was digested with the enzymes NotI and SalI and inserted into the respective sites of the vector G1BgSVNA (a retroviral vector encoding a hygromycin resistance gene and beta-galactosidase).
Proper citation: RRID:Addgene_16573 Copy
Species: Homo sapiens
Genetic Insert: CDK4 promoter
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:4360; Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10688915
Proper citation: RRID:Addgene_16571 Copy
Species: Homo sapiens
Genetic Insert: PUMA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:10400; Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11463391
Comments: Contains a 15-amino acid deletion encompassing the BH3 domain (AA 136–145).
Proper citation: RRID:Addgene_16589 Copy
Species: Homo sapiens
Genetic Insert: BAX alpha
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:10400; Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11463391
Proper citation: RRID:Addgene_16587 Copy
Species: Homo sapiens
Genetic Insert: c-myc promoter (4xTBE1 wt)
Vector Backbone Description: Backbone Marker:Vogelstein Lab; Backbone Size:4867; Vector Backbone:pBV-Luc; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9727977
Comments: Tcf-4 binding elements (TBEs). To construct the four tandem repeats of TBE1, we dimerized oligonucleotide cassettes containing two copies of the site and cloned the products into pBV-Luc (5'-CTAGCGCACCTTTGATTTCTGCACCTTTGATTTCTG-3' and 5'-CTAGCAGAAATCAAAGGTGCAGAAATCAAAGGTGCG-3')
Proper citation: RRID:Addgene_16597 Copy
Species: Homo sapiens
Genetic Insert: p53 binding sites
Vector Backbone Description: Backbone Marker:Vogelstein Lab; Backbone Size:4867; Vector Backbone:pBV-Luc; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11463391
Comments: The following oligo pairs containing two copies of wild-type p53 binding sites were used: 5'-CTAGGCTGCA AGTCCTGACT TGTCCACACT CTGCAAGTCC TGACTTGTCC-3' and 5'-CTAGGGACAA GTCAGGACTT GCAGAGTGTG GACAAGTCAG GACTTGCAGC-3'. The annealed oligonucleotide pairs were concatamerized and cloned into the NheI site of pBVLuc.
Proper citation: RRID:Addgene_16593 Copy
Species: Homo sapiens
Genetic Insert: PUMA promotor fragment
Vector Backbone Description: Backbone Marker:Vogelstein Lab; Backbone Size:4867; Vector Backbone:pBV-Luc; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11463391
Comments: Deletion of 5' terminal 300 bp from Frag1. Does not contain consensus p53 binding sites BS1 and BS2.
Proper citation: RRID:Addgene_16592 Copy
Species: Homo sapiens
Genetic Insert: HuT1head1-405(T144E,T150E)
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific, Carlsbad, CA; Vector Backbone:pTrcHis C; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33046605
Proper citation: RRID:Addgene_166130 Copy
Species: Homo sapiens
Genetic Insert: T1head1-405(del37/GAG)
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific, Carlsbad, CA; Vector Backbone:pTrcHis C; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33046605
Proper citation: RRID:Addgene_166129 Copy
Species: Homo sapiens
Genetic Insert: T1head1-405(del30)
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific, Carlsbad, CA; Vector Backbone:pTrcHis C; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33046605
Proper citation: RRID:Addgene_166128 Copy
Species: Homo sapiens
Genetic Insert: Tln1Head (1-405)
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific, Carlsbad, CA; Vector Backbone:pTrcHis C; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33046605
Proper citation: RRID:Addgene_166127 Copy
Species: Homo sapiens
Genetic Insert: PFAS-mCherry
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5472; Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32439803
Proper citation: RRID:Addgene_166024 Copy
Species: Homo sapiens
Genetic Insert: human equilibrative nucleoside transporter 1
Vector Backbone Description: Vector Backbone:pGK401red; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33758180
Comments: hENT1 gene was cloned from addgene plasmid pFS181 (#12536, Depositor: Nick Rhind)
Proper citation: RRID:Addgene_166028 Copy
Species: Homo sapiens
Genetic Insert: c-myc promoter (Frag B)
Vector Backbone Description: Backbone Marker:Vogelstein Lab; Backbone Size:4867; Vector Backbone:pBV-Luc; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9727977
Comments: See Author's Map and article for more information.
Proper citation: RRID:Addgene_16606 Copy
Species: Homo sapiens
Genetic Insert: FDXR knockout
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript II KS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11590433
Comments: See author's map for more information. 5' arm is 0.8 kb (-768 to -1) and 3' arm is 3.4 kb (+2051 to +5336).
Proper citation: RRID:Addgene_16613 Copy
Species: Homo sapiens
Genetic Insert: MnSOD
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5200; Vector Backbone:pBI-EGFP2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11590433
Comments: Contains full length MnSOD gene including the mitochondrial leader sequence.
Proper citation: RRID:Addgene_16612 Copy
Species: Homo sapiens
Genetic Insert: FDXR
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3800; Vector Backbone:pTRE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11590433
Comments: See author's map for cloning details.
Proper citation: RRID:Addgene_16611 Copy
Species: Homo sapiens
Genetic Insert: FDXR
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3800; Vector Backbone:pTRE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11590433
Comments: See author's map for cloning details.
Proper citation: RRID:Addgene_16610 Copy
Species: Homo sapiens
Genetic Insert: DNMT1 knock-out construct
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3264; Vector Backbone:pSL301; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11932749
Comments: 5' arm is 4.3 kb. 3' arm is 3.0 kb. See author's map for details.
Proper citation: RRID:Addgene_16648 Copy
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