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Species: Synthetic
Genetic Insert: Optimized G Protein
Vector Backbone Description: Vector Backbone:pAAV-syn-FLEX-splitTVA-eGFP-B19G IW; Vector Types:AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_85225 Copy
Species: Synthetic
Genetic Insert: EBFP2
Vector Backbone Description: Backbone Size:6417; Vector Backbone:LeGO vector; Vector Types:Mammalian Expression, Lentiviral, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28109958
Comments: HIV-1 derived third generation lentiviral vector for stable cell marking. Use second generation (like psPAX2) or third generation (like pMDLg/pRRE + pRSV-Rev) systems for packaging in addition to a VSV-G expressing plasmid (or another envelope protein). Please visit the LeGO-Vector home page for more information: http://www.LentiGO-Vectors.de
The BsrGI site of EBFP2 can be used to fuse an antibiotic resistance to the fluorescent protein following the building block principle of the LeGO vector system. More information is available on the LeGO website and in the following publications.
More references:
Weber et al., Molecular Therapy, 2008:
http://www.nature.com/mt/journal/v16/n4/full/mt20086a.html
Weber et al., Gene Therapy, 2010:
http://www.nature.com/gt/journal/v17/n4/full/gt2009149a.html
Proper citation: RRID:Addgene_85213 Copy
Species: Synthetic
Genetic Insert: EGFP
Vector Backbone Description: Vector Backbone:pRS402; Vector Types:Yeast Expression, integrating, shuttle vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22722195
Proper citation: RRID:Addgene_85190 Copy
Species: Synthetic
Genetic Insert: KRAB-dCas9-IRES-GFP
Vector Backbone Description: Backbone Marker:Addgene; Backbone Size:8812; Vector Backbone:pHR; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27708057
Proper citation: RRID:Addgene_85556 Copy
Species: Synthetic
Genetic Insert: superfolder GFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4100; Vector Backbone:pBAD/Myc-His A; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20082521
Proper citation: RRID:Addgene_85482 Copy
Species: Synthetic
Genetic Insert: Sp dCas9
Vector Backbone Description: Vector Backbone:pHAGE-DEST; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27984730
Comments: Multicolor CRISPR labeling of chromosomal loci in human cells. Ma H, Naseri A, Reyes-Gutierrez P, Wolfe SA, Zhang S, Pederson T. Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):3002-7. doi: 10.1073/pnas.1420024112. Epub 2015 Feb 23. 10.1073/pnas.1420024112 PubMed 25713381
Proper citation: RRID:Addgene_85717 Copy
Species: Synthetic
Genetic Insert: EGFP
Vector Backbone Description: Backbone Marker:Feng Zhang laboratory, MIT; Backbone Size:12992; Vector Backbone:plentiCRISPRv2; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33514835
Comments: This lenti-CRISPRv2 vector was used as control for studies with gene specific lenti-CRISPRv2 gene deletion of Plexin-B2 in human cells (plentiCRISPRv2-sgPLXNB2).
Plasmid received from Jialiang Liang, Laboratory of Patrizia Casaccia, Icahn School of Medicine at Mount Sinai
Proper citation: RRID:Addgene_86153 Copy
Species: Synthetic
Genetic Insert: p9-FLAG-FMO
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:CDF-Duet; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:27487319
Proper citation: RRID:Addgene_86479 Copy
Species: Synthetic
Genetic Insert: GFP
Vector Backbone Description: Backbone Size:6181; Vector Backbone:pMSCV; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27914197
Proper citation: RRID:Addgene_86537 Copy
Species: Synthetic
Genetic Insert: p9-6His-tnaA
Vector Backbone Description: Vector Backbone:pWW307; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27487319
Proper citation: RRID:Addgene_86481 Copy
Species: Synthetic
Genetic Insert: Pveg-sLucopt
Vector Backbone Description: Backbone Marker:Robert Fagan and Neil Fairweather; Backbone Size:6378; Vector Backbone:pRPF185; Vector Types:Bacterial Expression, Luciferase, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Comments: Please note that the individual components of this construct (that is, Pveg and sLucopt) are described in Oliveira Paiva AM et al (2016) ACS Synth Biol (DOI: 10.1021/acssynbio.6b00104). During the cloning a mutation occurred that removes the SacI site in between the Pveg and sLucopt genes. The 5' (KpnI) and 3' (BamHI) restriction sites remain.
Proper citation: RRID:Addgene_86554 Copy
Species: Synthetic
Genetic Insert: Cas9
Vector Backbone Description: Backbone Marker:Kristin Bernhardt; Backbone Size:11630; Vector Backbone:pKB65; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28174584
Comments: See Hahn et al., 2017
Proper citation: RRID:Addgene_86556 Copy
Species: Synthetic
Genetic Insert: CANE-LV envelope
Vector Backbone Description: Backbone Size:4821; Vector Backbone:pCASGS/ES; Vector Types:Mammalian Expression, Mouse Targeting, Lentiviral, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27974160
Proper citation: RRID:Addgene_86666 Copy
Species: Synthetic
Genetic Insert: MTST6
Vector Backbone Description: Backbone Marker:Genewiz; Backbone Size:2700; Vector Backbone:pUC57; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24060124
Proper citation: RRID:Addgene_86603 Copy
Species: Synthetic
Genetic Insert: ER-GCaMP6-210
Vector Backbone Description: Backbone Size:9246; Vector Backbone:FCK(1.3)GW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28162809
Proper citation: RRID:Addgene_86919 Copy
Species: Synthetic
Genetic Insert: mKO2-hCDT1(30-120)
Vector Backbone Description: Backbone Marker:Verma lab (Salk Institute); Backbone Size:7800; Vector Backbone:pBOB; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27888217
Proper citation: RRID:Addgene_86849 Copy
Species: Synthetic
Genetic Insert: NS3 1-177
Vector Backbone Description: Backbone Marker:Novagene; Backbone Size:5420; Vector Backbone:pETDUET-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27940580
Proper citation: RRID:Addgene_86846 Copy
Species: Synthetic
Genetic Insert: Cas9-venus
Vector Backbone Description: Backbone Size:2770; Vector Backbone:Bluescript; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30912045
Proper citation: RRID:Addgene_86986 Copy
Species: Synthetic
Genetic Insert: PCNA3 fused to phosphite dehydrogenase and P450cam
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5700; Vector Backbone:pET-15b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25733255
Proper citation: RRID:Addgene_86971 Copy
Species: Synthetic
Genetic Insert: PCNA1 fused to phosphite dehydrogenase and putidaredoxin reductase
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5700; Vector Backbone:pET-15b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25733255
Proper citation: RRID:Addgene_86969 Copy
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