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Species: Homo sapiens
Genetic Insert: STAT1 S727A
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16799645
Comments: The STAT1 full-size coding region was amplified using total cDNA from SK-NEP-1 cells and cloned into pEGFP-C1. S727A mutation made using QuikChange mutagenesis kit.
Proper citation: RRID:Addgene_12304 Copy
Species: Danio rerio
Genetic Insert: Fgf2
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16862139
Proper citation: RRID:Addgene_12309 Copy
Species: Synthetic
Genetic Insert: 2x35S::HcPro::tNOS 2x35S::fLUC-I::tNOS
Vector Backbone Description: Backbone Marker:Modified from pAGM4723 (Weber et al., 2011; DOI:10.1371/journal.pone.0016765) by the addition of the VirGN54D gene; Backbone Size:5851; Vector Backbone:pSB90; Vector Types:Plant Expression, Luciferase; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31263474
Comments: Transient fLUC and TEV HcPro (silencing suppressor) expression vector for plants. Introns avoid expression of fLUC in Agrobacteria. Increased virulence in Agro. through VirGN54D in the vector backbone
Proper citation: RRID:Addgene_123193 Copy
Species: Synthetic
Genetic Insert: 2x35S::ZCT1::tMAS
Vector Backbone Description: Backbone Marker:Modified from pAGM4723 (Weber et al., 2011; DOI:10.1371/journal.pone.0016765) by the addition of the VirGN54D gene; Backbone Size:5851; Vector Backbone:pSB90; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31263474
Comments: Vector for transient expression of ZCT1 from Chatharanthus roseus. ZCT1 is a repressor of the C. roseus STR1 promoter. Can be used in combination with pSB137 for transactivation/repression assays.
Proper citation: RRID:Addgene_123195 Copy
Species: Synthetic
Genetic Insert: pNOS::rLUC-I::tNOS tOCS::fLUC-I::pSTR1
Vector Backbone Description: Backbone Marker:Modified from pAGM4723 (Weber et al., 2011; DOI:10.1371/journal.pone.0016765) by the addition of the VirGN54D gene; Backbone Size:5851; Vector Backbone:pSB90; Vector Types:Plant Expression, Luciferase; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31263474
Comments: Transient fLUC and rLUC expression vector for plants. Introns avoid expression in Agrobacteria. Increased virulence in Agro. through VirGN54D in the vector backbone.
Proper citation: RRID:Addgene_123194 Copy
Species: Synthetic
Genetic Insert: 2x35S::GUS::tMAS
Vector Backbone Description: Backbone Marker:Modified from pAGM4723 (Weber et al., 2011; DOI:10.1371/journal.pone.0016765) by the addition of the VirGN54D gene; Backbone Size:5851; Vector Backbone:pSB90; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31263474
Comments: Vector for transient expression of GUS (contains two introns for avoiding expression in Agrobacteria). Small insert size and VirGN54D in the vector backbone facilitate strong transient expression in plants.
Proper citation: RRID:Addgene_123197 Copy
Species: Synthetic
Genetic Insert: tMAS::rLUC-I::pMAS
Vector Backbone Description: Backbone Marker:Modified from pAGM4723 (Weber et al., 2011; DOI:10.1371/journal.pone.0016765) by the addition of the VirGN54D gene; Backbone Size:5851; Vector Backbone:pSB90; Vector Types:Plant Expression, Luciferase; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31263474
Comments: Vector for transient expression of a Renilla luciferase (rLUC, intron for avoiding expression in Agrobacteria). Small insert size and VirGN54D in the vector backbone facilitate strong transient expression in plants.
Proper citation: RRID:Addgene_123199 Copy
Species: Synthetic
Genetic Insert: 2x35S::P19::tNOS 2x35S::fLUC-I::tNOS
Vector Backbone Description: Backbone Marker:Modified from pAGM4723 (Weber et al., 2011; DOI:10.1371/journal.pone.0016765) by the addition of the VirGN54D gene; Backbone Size:5851; Vector Backbone:pSB90; Vector Types:Plant Expression, Luciferase; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31263474
Comments: Transient fLUC and TBSV P19 (silencing suppressor) expression vector for plants. Introns avoid expression of fLUC in Agrobacteria. Increased virulence in Agrob. through VirGN54D in the vector backbone
Proper citation: RRID:Addgene_123191 Copy
Species: Synthetic
Genetic Insert: 2x35S::tGFP-I::tNOS 2x35S::fLUC-I::tNOS
Vector Backbone Description: Backbone Marker:Modified from pAGM4723 (Weber et al., 2011; DOI:10.1371/journal.pone.0016765) by the addition of the VirGN54D gene; Backbone Size:5851; Vector Backbone:pSB90; Vector Types:Plant Expression, Luciferase; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31263474
Comments: Transient fLUC and turboGFP expression vector for plants. Introns avoid expression in Agrobacteria. Increased virulence in Agrobacteria through VirGN54D in the vector backbone.
Proper citation: RRID:Addgene_123190 Copy
Species: Synthetic
Genetic Insert: 2x35S::fLUC-I::tNOS and VirGN54D in the vector backbone
Vector Backbone Description: Backbone Marker:Modified from pAGM4723 (Weber et al., 2011; DOI:10.1371/journal.pone.0016765) by the addition of the VirGN54D gene; Backbone Size:5874; Vector Backbone:pSB90; Vector Types:Plant Expression, Luciferase; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31263474
Comments: Transient fLUC expression vector for plants; Intron avoids expression in Agrobacteria; Increased virulence in Agrobacteria through VirGN54D in the vector backbone.
Proper citation: RRID:Addgene_123188 Copy
Species: Agrobacterium tumefaciens
Genetic Insert: VirGN54D from pAD1289 (a gift from A. Das)
Vector Backbone Description: Backbone Marker:Weber et al., 2011 (DOI:10.1371/journal.pone.0016765); Backbone Size:12773; Vector Backbone:pAGM4723; Vector Types:Plant Expression, Level 2 acceptor vector for expression in plants ; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31263474
Comments: Golden Gate (MoClo; Weber et al., 2011) level 2 acceptor vector with Mutation VirGN54D for increased virulence. Increased virulence in Agrobacteria for a high-efficiency gene transfer (increased with and without acetosyringone, compared to original vector control).
Proper citation: RRID:Addgene_123187 Copy
Species: Synthetic
Genetic Insert: J105-30arsR-t-ParsR-30gfp-t
Vector Backbone Description: Backbone Size:2750; Vector Backbone:pSB3K3; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30911179
Proper citation: RRID:Addgene_123162 Copy
Genetic Insert: Pecf11-30gfp-t
Vector Backbone Description: Backbone Size:2204; Vector Backbone:pSB1K3; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30911179
Proper citation: RRID:Addgene_123152 Copy
Species: Synthetic
Genetic Insert: J117-30arsR-t-ParsR-30gfp-t
Vector Backbone Description: Backbone Size:2750; Vector Backbone:pSB3K3; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30911179
Proper citation: RRID:Addgene_123142 Copy
Species: Synthetic
Genetic Insert: J109-32merR-t-PmerT-30gfp-t
Vector Backbone Description: Backbone Size:2750; Vector Backbone:pSB3K3; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30911179
Proper citation: RRID:Addgene_123148 Copy
Genetic Insert: PrinA-30gfp-t
Vector Backbone Description: Backbone Size:2204; Vector Backbone:pSB1K3; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30911179
Proper citation: RRID:Addgene_123147 Copy
Species: Mus musculus
Genetic Insert: ATG5
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3941; Vector Backbone:pEGFPC1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16645637
Comments: for mCherry marker protein
Campbell RE, Tour O, Palmer AE, Steinbach PA, Baird GS, Zacharias DA,
Tsien RY. Proc Natl Acad Sci U S A. 2002 Jun 11;99(12):7877-82
Proper citation: RRID:Addgene_13095 Copy
Species: Mus musculus
Genetic Insert: ATG5-K130R
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3941; Vector Backbone:pEGFPC1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16645637
Comments: for mCherry marker protein
Campbell RE, Tour O, Palmer AE, Steinbach PA, Baird GS, Zacharias DA, Tsien RY. Proc Natl Acad Sci U S A. 2002 Jun 11;99(12):7877-82
Proper citation: RRID:Addgene_13096 Copy
Species: Homo sapiens
Genetic Insert: KIF1C-GFP
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25344256
Proper citation: RRID:Addgene_130977 Copy
Species: Synthetic
Genetic Insert: sgRNA-LEB4
Vector Backbone Description: Backbone Marker:Designed by: Austin Che; Mutated by: unknown; Backbone Size:2204; Vector Backbone:pSB1K3mut; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31451697
Comments: The Top10 bacterial strain should be used for downstream applications
Proper citation: RRID:Addgene_130961 Copy
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