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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
P-rTetO-mCherry-T2A-RPL22-1xV5
 
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RRID:Addgene_170325 RPL22 Homo sapiens Ampicillin PMID:32516938 Vector Backbone:pLV; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin None 2026-08-15 01:10:07 0
P-rTetO-EGFP-T2A-RPL22-3xHA
 
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RRID:Addgene_170326 RPL22 Homo sapiens Ampicillin PMID:32516938 Vector Backbone:pLV; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin None 2026-08-15 01:10:07 0
pcDNA3.3_CoV1_D28
 
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10+ mentions
RRID:Addgene_170447 SARS-CoV-1 spike D28 SARS-CoV-1 Ampicillin PMID:32540903 Backbone Size:5500; Vector Backbone:pcDNA3.3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin last 28aa deletion in c-terminal tail 2026-08-15 01:10:08 11
pCDH-EF1a-hLMX1A-T2A-copGFP
 
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RRID:Addgene_170444 human LMX1A Ampicillin PMID:33597231 Backbone Marker:System Biosciences; Backbone Size:7424; Vector Backbone:pCDH-EF1a-T2A-copGFP; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin 2026-08-15 01:10:08 0
P-Ef1a-mCherry-T2A-RPL22-1xV5
 
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RRID:Addgene_170324 RPL22 Homo sapiens Ampicillin PMID:32516938 Vector Backbone:pFUGW; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin None 2026-08-15 01:10:07 0
pPD152.79
 
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RRID:Addgene_1704 Ampicillin See Fire Lab Vector Kit Documentation 2005. Fire Lab Miniprep Number pPD152.79, Ligation number L5494. Backbone Size:4771; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:10:07 0
pESUMO-hRORg2
 
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1+ mentions
RRID:Addgene_170329 RORG isoform 2 Homo sapiens Ampicillin PMID:34547329 Backbone Marker:LifeSensor; Backbone Size:5476; Vector Backbone:pESUMO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin codon optimized 2026-08-15 01:10:07 1
pET-DEST42-dre4
 
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RRID:Addgene_170440 dre4 Drosophila melanogaster Ampicillin PMID:31242411 The expressed his-tagged protein, pET-DEST42-dre4 (deleted amino acids 1-20) was efficiently bound to CNBR beads during the affinity purification of the antibodies. Backbone Marker:Invitrogen; Backbone Size:7440; Vector Backbone:pET-DEST42; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin Deleted amino acids 1-20 2026-08-15 01:10:08 0
pAC-CR7T-gRNA2.1-nlsBFP
 
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1+ mentions
RRID:Addgene_170515 Ampicillin PMID:33782117 For more information about the Han Lab Drosophila Transgenic Vectors, please visit: https://han.wicmb.cornell.edu/han-lab-drosophila-transgenic-vectors/ Backbone Size:12778; Vector Backbone:pAC; Vector Types:Insect Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:10:08 1
LentiGuide Cherry
 
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1+ mentions
RRID:Addgene_170510 S. pyogenes sgRNA cassette Synthetic Ampicillin Plasmid based on lentiGuide Puro (addgene plasmid #52963). Please visit https://www.biorxiv.org/content/10.1101/2021.03.02.433398v1 for bioRxiv preprint. Backbone Marker:Feng Zhang lab (Addgene plasmid #52963); Vector Backbone:lentiGuide-Puro; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:10:08 2
p663-UBC-miniTurbo-V5-PIP4K2C_IDG-K
 
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RRID:Addgene_170598 miniTurbo-V5-PIP4K2C Homo sapiens Ampicillin These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags. Backbone Marker:PEL; Backbone Size:9019; Vector Backbone:pDest663; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin 2026-08-15 01:10:09 0
p663-UBC-miniTurbo-V5-PIP5K1B_IDG-K
 
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RRID:Addgene_170599 miniTurbo-V5-PIP5K1B Homo sapiens Ampicillin These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags. Backbone Marker:PEL; Backbone Size:9019; Vector Backbone:pDest663; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin 2026-08-15 01:10:09 0
p663-UBC-miniTurbo-V5-PIK3C2G_IDG-K
 
Resource Report
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RRID:Addgene_170597 miniTurbo-V5-PIK3C2G Homo sapiens Ampicillin These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags. Backbone Marker:PEL; Backbone Size:9019; Vector Backbone:pDest663; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin 2026-08-15 01:10:10 0
ZE11 tiggywinkle hedgehog CMV
 
Resource Report
Resource Website
RRID:Addgene_17060 tiggywinkle hedgehog Danio rerio Ampicillin twhh under control of CMV promoter. Backbone Size:0; Vector Backbone:NA; Vector Types:; Bacterial Resistance:Ampicillin 2026-08-15 01:10:09 0
pXL-BacII-CAG-mCherry
 
Resource Report
Resource Website
RRID:Addgene_170519 CAG-mCherry Synthetic Ampicillin PMID:34458399 More information can be found in this additional reference: Yamagata M, Yan W, Sanes JR. A cell atlas of the chick retina based on single-cell transcriptomics. Elife. 2021 Jan 4;10:e63907. doi: 10.7554/eLife.63907. PMID: 33393903; PMCID: PMC7837701. Vector Backbone:pXL-BacII; Vector Types:Mammalian Expression, piggyBAC transposon; Bacterial Resistance:Ampicillin 2026-08-15 01:10:08 0
ZE19 wnt8 pBand3-5
 
Resource Report
Resource Website
RRID:Addgene_17053 wnt8 pBand3-5 Danio rerio Ampicillin PMID:11703928 RT PCR prodct ligated into vector full length cDNA cloned includes ORF1, IRES and ORF2. For antiense RNA: transcribe with SP6 polymerase. Plasmid History: Species of Sequence Origin: Zebrafish. Reference: Lekven, Dev Cell, 2001. Backbone Marker:Promega; Backbone Size:3000; Vector Backbone:pGemT easy; Vector Types:; Bacterial Resistance:Ampicillin 2026-08-15 01:10:08 0
ZE7 Pax2
 
Resource Report
Resource Website
RRID:Addgene_17056 pax b Danio rerio Ampicillin PMID:7586754 Insert: pax b sense or antisense orientation. Insert size: 1.18 Kb. Transcription: linearize at EcoR1, transcribe with SP6. Plasmid History: Constructed by Greg Kelly on date of 9/21/92, page 118 Book 1. Sepecies of Sequence Origin: Zebrafish. Note for Use: This construct is used to make DIG probes or for making RNA suitable for injections. Citation: Kelly and Moon, Developmental Genetics (1995) Backbone Size:3000; Vector Backbone:SP64T; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:10:09 0
p663-UBC-miniTurbo-V5-MKNK2_IDG-K
 
Resource Report
Resource Website
RRID:Addgene_170594 miniTurbo-V5-MKNK2 Homo sapiens Ampicillin These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags. Backbone Marker:PEL; Backbone Size:9019; Vector Backbone:pDest663; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin 2026-08-15 01:10:10 0
p663-UBC-miniTurbo-V5-EEF2K_IDG-K
 
Resource Report
Resource Website
RRID:Addgene_170592 miniTurbo-V5-EEF2K Homo sapiens Ampicillin These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags. Backbone Marker:PEL; Backbone Size:9019; Vector Backbone:pDest663; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin 2026-08-15 01:10:09 0
p663-UBC-miniTurbo-V5-MKNK1_IDG-K
 
Resource Report
Resource Website
RRID:Addgene_170593 miniTurbo-V5-MKNK1 Homo sapiens Ampicillin These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags. Backbone Marker:PEL; Backbone Size:9019; Vector Backbone:pDest663; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin 2026-08-15 01:10:09 0

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