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Species: Synthetic
Genetic Insert: TetR-GFP
Vector Backbone Description: Backbone Size:6805; Vector Backbone:pMF272; Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31821884
Proper citation: RRID:Addgene_131319 Copy
Species: Synthetic
Genetic Insert: Full-length tetO array (XbaI-NheI fragment of pLAU44)
Vector Backbone Description: Backbone Size:6803; Vector Backbone:pMF272; Vector Types:Unspecified; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:31821884
Comments: TetO arrays are unstable and dual selection with Ampicillin and Gentamycin are essential for the maintenance of the array. Confirm full-length plasmid by restriction digest and Sanger sequencing of any clones prior to use.
Some minor sequence discrepancies located in the TetO repeat array exist between Sanger and NGS based data. These are not of functional concern for use of the plasmid.
Proper citation: RRID:Addgene_131318 Copy
Species: Synthetic
Genetic Insert: Codon optimized SpCas9-NG
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31501500
Comments: This plasmid vector was constructed by mutation PCR of pCAG-T3-hCAS9-pA plasmid vector (Addgene plasmid 48625) according to the amino acid sequences of SpCas9-NG shown in previous study (Nishimasu H et al., Engineered CRISPR-Cas9 nuclease with expanded targeting space. Science, 2018).
Proper citation: RRID:Addgene_131465 Copy
Species: Synthetic
Genetic Insert: Codon optimized eSpCas9-NG
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31501500
Comments: This plasmid vector was constructed by mutation PCR of pCAG-T3-hCAS9-pA plasmid vector (Addgene plasmid 48625) according to the amino acid sequences of SpCas9-NG shown in previous study (Nishimasu H et al., Engineered CRISPR-Cas9 nuclease with expanded targeting space. Science, 2018) with high-fidelity eSp variant shown in previous study (Slaymaker IM et al, Rationally engineered Cas9 nucleases with improved specificity. Science, 2016).
Proper citation: RRID:Addgene_131466 Copy
Species: Synthetic
Genetic Insert: Streptomyces codon optimized spCas9n (D10A)
Vector Backbone Description: Backbone Size:6971; Vector Backbone:pGM1190; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Apramycin
Defining Citation: PMID:31548381
Proper citation: RRID:Addgene_131464 Copy
Species: Synthetic
Genetic Insert: Codon optimized st1Cas9
Vector Backbone Description: Vector Backbone:pCAG-T3-hCAS9-pA; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27318086
Proper citation: RRID:Addgene_131542 Copy
Species: Synthetic
Genetic Insert: CjCas9
Vector Backbone Description: Vector Backbone:pCAG-T3-hCAS9-pA; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29088065
Proper citation: RRID:Addgene_131543 Copy
Species: Synthetic
Genetic Insert: RT-1306
Vector Backbone Description: Backbone Size:5211; Vector Backbone:pET30; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31548705
Proper citation: RRID:Addgene_131521 Copy
Species: Synthetic
Genetic Insert: Mastigocladus laminosus ferredoxin
Vector Backbone Description: Backbone Size:2909; Vector Backbone:ColE1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Comments: Please visit https://www.biorxiv.org/content/10.1101/645978v1 for BioRxiv preprint
Proper citation: RRID:Addgene_131591 Copy
Species: Synthetic
Genetic Insert: GFP-2-cut reporter
Vector Backbone Description: Backbone Size:8700; Vector Backbone:pFUGW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31363085
Proper citation: RRID:Addgene_131669 Copy
Species: Synthetic
Genetic Insert: ZmUBIp::SpCas9 (wheat codon optimized, version 2):NOSt
Vector Backbone Description: Vector Backbone:pICH47742; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32326901
Proper citation: RRID:Addgene_131765 Copy
Species: Synthetic
Genetic Insert: sTRSV
Vector Backbone Description: Vector Backbone:pCS408; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31548547
Proper citation: RRID:Addgene_131744 Copy
Species: Synthetic
Genetic Insert: sTRSV ctl
Vector Backbone Description: Vector Backbone:pCS408; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31548547
Proper citation: RRID:Addgene_131745 Copy
Species: Synthetic
Genetic Insert: c-di-GMP-II switch cdGII-CGUAA
Vector Backbone Description: Vector Backbone:pCS4076; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31548547
Proper citation: RRID:Addgene_131743 Copy
Species: Synthetic
Genetic Insert: M. laminosus Fd1 mutant with the estrogen recepor-ligand binding domain fused between amino acids 54 and 55
Vector Backbone Description: Vector Backbone:ColE1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Comments: For detailed annotations see the GenBank file under Resource Information/Supplemental Documents. Please visit https://www.biorxiv.org/content/10.1101/645978v1 for BioRxiv preprint
Proper citation: RRID:Addgene_131816 Copy
Species: Synthetic
Genetic Insert: M. laminosus Fd1 mutant with the estrogen recepor-ligand binding domain fused between amino acids 55 and 56
Vector Backbone Description: Vector Backbone:ColE1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Comments: For detailed annotations see the GenBank file under Resource Information/Supplemental Documents. Please visit https://www.biorxiv.org/content/10.1101/645978v1 for BioRxiv preprint
Proper citation: RRID:Addgene_131817 Copy
Species: Synthetic
Genetic Insert: M. laminosus Fd1 mutant with the estrogen recepor-ligand binding domain fused between amino acids 54 and 55
Vector Backbone Description: Vector Backbone:ColE1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Comments: For detailed annotations see the GenBank file under Resource Information/Supplemental Documents. Please visit https://www.biorxiv.org/content/10.1101/645978v1 for BioRxiv preprint
Proper citation: RRID:Addgene_131815 Copy
Species: Synthetic
Genetic Insert: rabies glycoprotein - oG
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:5571; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV, INTRSECT; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31553913
Proper citation: RRID:Addgene_131778 Copy
Species: Synthetic
Genetic Insert: M. laminosus Fd1 mutant with the estrogen recepor-ligand binding domain fused between amino acids 30 and 31
Vector Backbone Description: Vector Backbone:ColE1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Comments: For detailed annotations see the GenBank file under Resource Information/Supplemental Documents. Please visit https://www.biorxiv.org/content/10.1101/645978v1 for BioRxiv preprint
Proper citation: RRID:Addgene_131811 Copy
Species: Synthetic
Genetic Insert: PADH1_FUSN_Fusion Red_Cry2Olig_TACT1
Vector Backbone Description: Vector Backbone:Yeast Expression; Vector Types:Yeast Expression, Integration into δ-sites; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31086330
Proper citation: RRID:Addgene_131770 Copy
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