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Species: Homo sapiens
Genetic Insert: hU6-gRNA and hH1-gRNA targeting cldn1 gene
Vector Backbone Description: Backbone Marker:Addgene #122182; Vector Backbone:pLenti spCas9 T2A iRFP670 P2A puro; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35388524
Proper citation: RRID:Addgene_166133 Copy
Species: Homo sapiens
Genetic Insert: PIK3CA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4856; Vector Backbone:pFastBac HT; Vector Types:Mammalian Expression, Baculoviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15016963
Comments: A fragment of human PIK3CA cDNA including the entire ORF was cloned into the pFastBac HT vector. The cDNA fragment can be removed with BamHI and HindIII for subcloning into other vectors.
Proper citation: RRID:Addgene_16644 Copy
Species: Homo sapiens
Genetic Insert: PIK3CA H1047R
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4856; Vector Backbone:pFastBac HT; Vector Types:Mammalian Expression, Baculoviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15016963
Comments: A fragment of human PIK3CA cDNA including the entire ORF was cloned into the pFastBac HT vector. The cDNA fragment can be removed with BamHI and HindIII for subcloning into other vectors.
Proper citation: RRID:Addgene_16641 Copy
Species: Homo sapiens
Genetic Insert: shRNA for human YAP
Vector Backbone Description: Vector Backbone:PLKO.1; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33606996
Proper citation: RRID:Addgene_166485 Copy
Species: Homo sapiens
Genetic Insert: DNMT3b knock-out construct
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3264; Vector Backbone:pSL301; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11932749
Comments: 5' arm is 3.0 kb. 3' arm is 4.6 kb. See author's map for details.
Proper citation: RRID:Addgene_16651 Copy
Species: Homo sapiens
Genetic Insert: DNMT3b knock-out construct
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3264; Vector Backbone:pSL301; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11932749
Comments: 5' arm is 3.0 kb. 3' arm is 4.6 kb. See author's map for details.
Proper citation: RRID:Addgene_16650 Copy
Species: Homo sapiens
Genetic Insert: hPOT1
Vector Backbone Description: Backbone Size:5665; Vector Backbone:pWZL N-MYC; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33122293
Proper citation: RRID:Addgene_166407 Copy
Species: Homo sapiens
Genetic Insert: hPOT1
Vector Backbone Description: Backbone Size:5665; Vector Backbone:pWZL N-MYC; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33122293
Proper citation: RRID:Addgene_166406 Copy
Species: Homo sapiens
Genetic Insert: BUB1 S492Y
Vector Backbone Description: Backbone Marker:Vogelstein Lab; Backbone Size:5099; Vector Backbone:pBI-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9521327
Comments: pBI-GFP-Bub1 S492Y was constructed by cloning RT-PCR products from tumour line V429 into the NotI and SalI sites of pBI-GFP.
Proper citation: RRID:Addgene_16625 Copy
Species: Homo sapiens
Genetic Insert: PTPRT R632X
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5472; Vector Backbone:pCI-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15155950
Comments: A 6kb fragment of PTPRT including the entire ORF and the 3'UTR was excised from KIAA clone (ha06139s1) with SgrAI and ScaI and cloned into the XmaI/EcoRV sites of pIRES2hyg2. A 1.9kb fragment containing PTPRT was digested with NheI and NotI and cloned into the pCI-Neo expression vector.
Proper citation: RRID:Addgene_16629 Copy
Species: Homo sapiens
Genetic Insert: PIK3CA H1047R
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4300; Vector Backbone:pCMV2-Tag 2A; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15016963
Comments: This plasmid has 5 additional mutations in PIK3CA compared to NP_006209.2: N114D, L177S, T322A, A400V, L404P. These mutations do not alter the function of the encoded proteins.
Proper citation: RRID:Addgene_16639 Copy
Species: Homo sapiens
Genetic Insert: PTPRT
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5472; Vector Backbone:pCI-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15155950
Comments: A 6kb fragment of PTPRT including the entire ORF and the 3'UTR was excised from KIAA clone (ha06139s1) with SgrAI and ScaI and cloned into the XmaI/EcoRV sites of pIRES2hyg2. The fragment containing PTPRT was digested with NheI and NotI and cloned into the pCI-Neo expression vector.
Proper citation: RRID:Addgene_16630 Copy
Species: Homo sapiens
Genetic Insert: mEGFP, YAPS127A-deltaCC
Vector Backbone Description: Vector Backbone:mEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33606996
Proper citation: RRID:Addgene_166459 Copy
Species: Homo sapiens
Genetic Insert: mCherry, TEAD4-YBD(217-434)
Vector Backbone Description: Vector Backbone:mCherry-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33606996
Proper citation: RRID:Addgene_166450 Copy
Species: Homo sapiens
Genetic Insert: mCherry, TEAD4-IDR
Vector Backbone Description: Vector Backbone:pet22b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33606996
Proper citation: RRID:Addgene_166442 Copy
Species: Homo sapiens
Genetic Insert: FDXR knockout
Vector Backbone Description: Backbone Marker:Vogelstein Lab; Backbone Size:3838; Vector Backbone:pFredA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11590433
Comments: 4.2 kb 5' targeting element (up to +1527). See author's map for more information.
Proper citation: RRID:Addgene_16614 Copy
Species: Homo sapiens
Genetic Insert: Cry2, mCherry, YAP-C-IDR(266-504)
Vector Backbone Description: Vector Backbone:pHAGE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33606996
Proper citation: RRID:Addgene_166447 Copy
Species: Homo sapiens
Genetic Insert: Cry2, mCherry, YAP-N-IDR(1-164)
Vector Backbone Description: Vector Backbone:pHAGE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33606996
Proper citation: RRID:Addgene_166446 Copy
Species: Homo sapiens
Genetic Insert: YAP4LE
Vector Backbone Description: Vector Backbone:pet25b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33606996
Proper citation: RRID:Addgene_166445 Copy
Species: Homo sapiens
Genetic Insert: Cry2, mCherry, TEAD4-IDR(89-230)
Vector Backbone Description: Vector Backbone:pHAGE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33606996
Proper citation: RRID:Addgene_166449 Copy
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