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Species: Synthetic
Genetic Insert: PCNA2 variant (L171C) fused to phosphite dehydrogenase and putidaredoxin
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5700; Vector Backbone:pET-15b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25733255
Proper citation: RRID:Addgene_86968 Copy
Species: Synthetic
Genetic Insert: NADPH Alanine Dehydrogenase
Vector Backbone Description: Backbone Marker:Lucigen (Middleton, WI); Backbone Size:1800; Vector Backbone:pSMART-HCKan (AF532107.1); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34600151
Comments: Read the preprint at bioRxiv: https://www.biorxiv.org/content/10.1101/2020.08.30.274290v1.full.
Proper citation: RRID:Addgene_87172 Copy
Species: Synthetic
Genetic Insert: U6-rMertksgRNA-Mertk(intron1-2)-nEF-GFPKASH-pA
Vector Backbone Description: Backbone Marker:Addgene 60957; Vector Backbone:PX551; Vector Types:AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27851729
Comments: There is 11bp deletion in left ITR, which is from original backbone plasmid (i.e. PX551). We don't believe it interfered with packaging.
Proper citation: RRID:Addgene_87119 Copy
Species: Synthetic
Genetic Insert: U6-Ai14sgRNA-Luciferase-nEF-GFPKASH-hGHpA
Vector Backbone Description: Backbone Marker:Addgene 60957; Vector Backbone:PX551; Vector Types:AAV, CRISPR, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27851729
Comments: There is 11bp deletion in left ITR, which is from original backbone plasmid (i.e. PX551). We don't believe it interfered with packaging.
Proper citation: RRID:Addgene_87118 Copy
Species: Synthetic
Genetic Insert: U6-mTubb3sgRNA-GFP-EF1a-mCherryKASH-hGHpA
Vector Backbone Description: Backbone Marker:Addgene 60957; Vector Backbone:PX551; Vector Types:AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27851729
Comments: There is 11bp deletion in left ITR, which is from original backbone plasmid (i.e. PX551). We don't believe it interfered with packaging.
Proper citation: RRID:Addgene_87116 Copy
Species: Synthetic
Genetic Insert: mcherry
Vector Backbone Description: Backbone Size:2000; Vector Backbone:pCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Proper citation: RRID:Addgene_87144 Copy
Species: Synthetic
Genetic Insert: mTagBFP2
Vector Backbone Description: Backbone Size:9926; Vector Backbone:pLenti-CaMKIIa-GFP; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_87254 Copy
Species: Synthetic
Genetic Insert: YFP-mAID-3HA
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2925; Vector Backbone:pBluescript SK(+); Vector Types:Toxoplasma expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28465425
Proper citation: RRID:Addgene_87259 Copy
Species: Synthetic
Genetic Insert: Split-Broccoli system (promoter control)
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28548488
Proper citation: RRID:Addgene_87315 Copy
Species: Synthetic
Genetic Insert: Split-Broccoli system "Bottom" transcript
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28548488
Proper citation: RRID:Addgene_87310 Copy
Species: Synthetic
Genetic Insert: Split-Broccoli system "Bottom" transcript
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28548488
Proper citation: RRID:Addgene_87313 Copy
Species: Synthetic
Genetic Insert: Split-Broccoli system
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28548488
Proper citation: RRID:Addgene_87314 Copy
Species: Synthetic
Genetic Insert: Split-Broccoli system "Top" transcript
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28548488
Proper citation: RRID:Addgene_87312 Copy
Species: Synthetic
Genetic Insert: GdT
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Backbone Size:4076; Vector Backbone:pBad His B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27781394
Proper citation: RRID:Addgene_87361 Copy
Species: Synthetic
Genetic Insert: GGvT
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Backbone Size:4076; Vector Backbone:pBad His B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27781394
Proper citation: RRID:Addgene_87362 Copy
Species: Synthetic
Genetic Insert: Three-way junction dimeric Broccoli aptamer
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28548488
Proper citation: RRID:Addgene_87308 Copy
Species: Synthetic
Genetic Insert: Split-Broccoli system "Top" transcript
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28548488
Proper citation: RRID:Addgene_87309 Copy
Species: Synthetic
Genetic Insert: HD-12
Vector Backbone Description: Backbone Marker:Life Technologies; Vector Backbone:pMA-T; Vector Types:TALEN, Synthetic Biology, TALE DBD library preparation; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27615025
Proper citation: RRID:Addgene_87469 Copy
Species: Synthetic
Genetic Insert: HD-3
Vector Backbone Description: Backbone Marker:Life Technologies; Vector Backbone:pMA-T; Vector Types:TALEN, Synthetic Biology, TALE DBD library preparation; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27615025
Proper citation: RRID:Addgene_87460 Copy
Species: Synthetic
Genetic Insert: HD-6
Vector Backbone Description: Backbone Marker:Life Technologies; Vector Backbone:pMA-T; Vector Types:TALEN, Synthetic Biology, TALE DBD library preparation; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27615025
Proper citation: RRID:Addgene_87463 Copy
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