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Species: Other
Genetic Insert: GRE-CYC1-lacZ
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:3043665
Comments: GRE is 5-AGTAGCTAGAACATCCTGTAC
Proper citation: RRID:Addgene_108218 Copy
Species: Other
Genetic Insert: Archon1-EGFP
Vector Backbone Description: Vector Backbone:pAAV-CaMKII; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29483642
Proper citation: RRID:Addgene_108417 Copy
Species: Other
Genetic Insert: Knockout cassette G418
Vector Backbone Description: Backbone Size:2071; Vector Backbone:pDM304; Vector Types:Cre/Lox, Dictyostelium discoideum knockout vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29847546
Comments: Due to difficulties in assembling repetitive elements within the plasmid, Addgene NGS may have some ambiguous bases and show minor differences compared to the depositor sequence. Please use the assembled depositor sequence if necessary.
Proper citation: RRID:Addgene_108982 Copy
Species: Other
Genetic Insert: Knockout cassette Nourseothricin
Vector Backbone Description: Backbone Size:2071; Vector Backbone:pDM304; Vector Types:Cre/Lox, Dictyostelium discoideum knockout vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29847546
Comments: Due to difficulties in assembling repetitive elements within the plasmid, Addgene NGS may have some ambiguous bases and show minor differences compared to the depositor sequence. Please use the assembled depositor sequence if necessary.
Proper citation: RRID:Addgene_108980 Copy
Species: Other
Genetic Insert: Cre recombinase
Vector Backbone Description: Backbone Size:6901; Vector Backbone:pDM1203; Vector Types:Cre/Lox, Cre expression vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29847546
Comments: Due to difficulties in assembling repetitive elements within the plasmid, Addgene NGS may have some ambiguous bases and show minor differences compared to the depositor sequence. Please use the assembled depositor sequence if necessary.
Proper citation: RRID:Addgene_108995 Copy
Species: Other
Genetic Insert: PTSG_07215
Vector Backbone Description: Vector Backbone:pDSB_0093; Vector Types:Choanoflagellate Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30281390
Comments: pDSB_0101 . Please visit https://www.biorxiv.org/content/early/2018/06/09/343111 for bioRxiv preprint.
Proper citation: RRID:Addgene_109100 Copy
Species: Other
Genetic Insert: pActAvrII_nanoluc
Vector Backbone Description: Vector Backbone:pDSB_0028; Vector Types:Choanoflagellate Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30281390
Comments: pDSB_0029 . Please visit https://www.biorxiv.org/content/early/2018/06/09/343111 for bioRxiv preprint.
Proper citation: RRID:Addgene_109077 Copy
Species: Other
Genetic Insert: PTSG_06009
Vector Backbone Description: Vector Backbone:pDSB_0093; Vector Types:Choanoflagellate Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30281390
Comments: pDSB_0099 . Please visit https://www.biorxiv.org/content/early/2018/06/09/343111 for bioRxiv preprint.
Proper citation: RRID:Addgene_109102 Copy
Species: Other
Genetic Insert: PTSG_07215 deltaCC
Vector Backbone Description: Vector Backbone:pDSB_0093; Vector Types:Choanoflagellate Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30281390
Comments: pDSB_0109 . Please visit https://www.biorxiv.org/content/early/2018/06/09/343111 for bioRxiv preprint.
Proper citation: RRID:Addgene_109107 Copy
Species: Other
Genetic Insert: GFP
Vector Backbone Description: Backbone Marker:from PMID 9218713; Vector Backbone:pSUM36; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22820329
Comments: GFP, originally modified for expression in prokaryotes and including an optimized translational initiation region from the plasmid pGreenTIR, was cloned from pGreenTIR into the HindIII site of the pSUM36 vector to generate pSum-LacZ-gfp.
Proper citation: RRID:Addgene_109285 Copy
Species: Other
Genetic Insert: GroEL-GFP
Vector Backbone Description: Backbone Marker:from PMID 9218713; Vector Backbone:pSUM36; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22820329
Comments: The groEL promoter was PCR-amplified from M. tuberculosis genomic DNA, cloned into the pCR2.1 TOPO-TA cloning vector and subsequently isolated as a BamHI/XbaI piece that was subcloned into pSUM36 cut with BamHI and NheI to remove the lacZ promoter. The GFP gene was subcloned into the HindIII site of the pSUM-groEL vector.
Proper citation: RRID:Addgene_109286 Copy
Species: Other
Genetic Insert: GFP
Vector Backbone Description: Backbone Marker:from PMID 9218713; Vector Backbone:pSUM36; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22820329
Comments: MCS2: HindIII and BamHI cloning sites
Proper citation: RRID:Addgene_109290 Copy
Species: Other
Genetic Insert: N-terminal fragment of Venus protein
Vector Backbone Description: Vector Backbone:pTOP-V2; Vector Types:tagging proteins in Cryptococcus neoformans; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:27780958
Proper citation: RRID:Addgene_122435 Copy
Species: Other
Genetic Insert: C-terminal fragment of Venus protein
Vector Backbone Description: Vector Backbone:pTOP-V2; Vector Types:tagging proteins in Cryptococcus neoformans; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:27780958
Proper citation: RRID:Addgene_122434 Copy
Species: Other
Genetic Insert: M13 bacteriophage genes
Vector Backbone Description: Vector Backbone:pBT13; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31332326
Comments: low copy vector, confers a growth defect
Please visit https://benchling.com/s/vGj0lSRM for additional information.
Proper citation: RRID:Addgene_122596 Copy
Species: Other
Genetic Insert: T7 RNA polymerase
Vector Backbone Description: Vector Backbone:pBT21; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31332326
Comments: Please visit https://benchling.com/s/GBYnBWvd for additional information.
Proper citation: RRID:Addgene_122595 Copy
Species: Other
Genetic Insert: M13 bacteriophage genes
Vector Backbone Description: Vector Backbone:pBT29; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31332326
Comments: low copy vector, confers a growth defect
Please visit https://benchling.com/s/EVGmG9HV for additional information.
Proper citation: RRID:Addgene_122598 Copy
Species: Other
Genetic Insert: gIII, luxAB, guide RNA
Vector Backbone Description: Vector Backbone:pBT120; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31332326
Comments: Please see https://benchling.com/s/seq-I9q2W3bzpjsuOoxQPyCj for additional information.
Proper citation: RRID:Addgene_122604 Copy
Species: Other
Genetic Insert: FERNY
Vector Backbone Description: Vector Backbone:pBT100; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31332326
Comments: Please see https://benchling.com/s/seq-hAPWUVQvZxeovBYRMQ0Q for additional information.
Proper citation: RRID:Addgene_122603 Copy
Species: Other
Genetic Insert: M13 bacteriophage genes
Vector Backbone Description: Vector Backbone:pBT137; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31332326
Comments: low copy vector, confers a growth defect
Please see https://benchling.com/s/seq-xGxHsGdVAtUUgfQsV2La for additional information.
Proper citation: RRID:Addgene_122606 Copy
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