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Species: Homo sapiens
Genetic Insert: SCN1A Variant 1, stabilized
Vector Backbone Description: Backbone Size:6053; Vector Backbone:pIR-CMV-IRES-mScarlet; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33460646
Comments: The SCN1A channel cDNA has been altered to be stably maintained in standard E.coli strains at high copy under standard growth conditions. Please visit https://www.biorxiv.org/content/10.1101/2020.11.05.370429v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_162278 Copy
Species: Homo sapiens
Genetic Insert: WDR91
Vector Backbone Description: Backbone Marker:SGC (Addgene Plasmid #62304); Vector Backbone:pFBOH-MHL; Vector Types:Baculovirus Expresssion; Bacterial Resistance:Ampicillin
Comments: N-terminal tag AA:MHHHHHHSSGRENLYFQG.
SGC Clone ID: WDR91:PBC019-H10:C244404.
PDB 6VYC: http://www.thesgc.org/structures/6VYC/
PDB 8SHJ: https://www.rcsb.org/structure/8SHJ/
PDB 8T55: https://www.rcsb.org/structure/8T55/
Proper citation: RRID:Addgene_162266 Copy
Vector Backbone Description: Backbone Marker:Dr. Jiri Zahradnik; Backbone Size:5209; Vector Backbone:New backbone; Vector Types:Yeast Expression, Shuttle vector bacteria/yeast; Bacterial Resistance:Kanamycin
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.12.16.423176v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_162450 Copy
Vector Backbone Description: Backbone Marker:Dr. Jiri Zahradnik; Backbone Size:5230; Vector Backbone:New backbone; Vector Types:Shuttle vector bacteria/yeast; Bacterial Resistance:Kanamycin
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.12.16.423176v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_162452 Copy
Species: Homo sapiens
Genetic Insert: p21
Vector Backbone Description: Backbone Size:0; Vector Backbone:pMT5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11231573
Comments: Order of elements: CMV promoter--Flag--NdeI--ATG--human p21--TGA--BamHI.
Proper citation: RRID:Addgene_16242 Copy
Species: Homo sapiens
Genetic Insert: p21
Vector Backbone Description: Backbone Size:5446; Vector Backbone:pMT5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11231573
Comments: Order of elements: CMV promoter--Flag--NdeI--ATG--human p21--TGA--BamHI.
Proper citation: RRID:Addgene_16240 Copy
Species: Homo sapiens
Genetic Insert: ASCL1
Vector Backbone Description: Backbone Size:8000; Vector Backbone:Tet-O-FUW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33264623
Proper citation: RRID:Addgene_162345 Copy
Species: Homo sapiens
Genetic Insert: MDM2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11715018
Proper citation: RRID:Addgene_16236 Copy
Species: R. norvegicus (rat), G. gallus (chicken); A. victoria (jellyfish)
Genetic Insert: jGCaMP8f
Vector Backbone Description: Vector Backbone:AAV-Syn; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Comments: For additional description and application data, see Zhang & Rózsa et al. (2023). Fast and sensitive GCaMP calcium indicators for imaging neural populations. Nature, 615(7954), 884–891. https://doi.org/10.1038/s41586-023-05828-9.
Proper citation: RRID:Addgene_162376 Copy
Species: R. norvegicus (rat), G. gallus (chicken); A. victoria (jellyfish)
Genetic Insert: jGCaMP8m
Vector Backbone Description: Backbone Marker:Scott Sternson; Vector Backbone:AAV-Syn-FLEX; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Comments: For additional description and application data, see Zhang & Rózsa et al. (2023). Fast and sensitive GCaMP calcium indicators for imaging neural populations. Nature, 615(7954), 884–891. https://doi.org/10.1038/s41586-023-05828-9.
Proper citation: RRID:Addgene_162378 Copy
Species: Synthetic
Genetic Insert: Lyn-YFP-FRB1N
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32747768
Proper citation: RRID:Addgene_162431 Copy
Species: R. norvegicus (rat), G. gallus (chicken); A. victoria (jellyfish)
Genetic Insert: jGCaMP8m
Vector Backbone Description: Backbone Marker:Gerald Rubin; Vector Backbone:derived from pJFRC7; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Comments: For additional description and application data, see Zhang & Rózsa et al. (2023). Fast and sensitive GCaMP calcium indicators for imaging neural populations. Nature, 615(7954), 884–891. https://doi.org/10.1038/s41586-023-05828-9.
Proper citation: RRID:Addgene_162387 Copy
Species: Homo sapiens
Genetic Insert: BRCA2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11888897
Comments: To construct p236BRCA2, the pcDNA3 vector was first modified by inserting a 236-bp fragment of the 5' untranslated region of BRCA2 between the KpnI and NotI sites. The assembled full-length BRCA2 cDNA was then inserted at the XhoI site of this plasmid. The 5' UTR of BRCA2 was obtained by RT-PCR using primers 5'-GGTACCGGTG GCGCGAGCTT CTGA-3' and 5'-GCGGCCGCAA CTACGATATT CCTCCAAT-3'.
Proper citation: RRID:Addgene_16246 Copy
Genetic Insert: Aga2p-eUnaG2
Vector Backbone Description: Backbone Marker:Dr. Jiri Zahradnik; Backbone Size:5254; Vector Backbone:New backbone pJYD; Vector Types:Shuttle vector bacteria/yeast; Bacterial Resistance:Kanamycin
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.12.16.423176v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_162457 Copy
Vector Backbone Description: Backbone Marker:Dr. Jiri Zahradnik; Backbone Size:5169; Vector Backbone:New backbone pJYD; Vector Types:Shuttle vector bacteria/yeast; Bacterial Resistance:Kanamycin
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.12.16.423176v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_162458 Copy
Species: Ciona intestinalis
Genetic Insert: VSFP2.1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5317; Vector Backbone:pcDNA3.1 (-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17487283
Comments: Voltage sensor domain of Ci-VSP fused to a pair of fluorescent proteins consisting of a C-terminally truncated CFP (Cerulean) and YFP (Citrine)
Proper citation: RRID:Addgene_16255 Copy
Species: Synthetic
Genetic Insert: ABE7.10-P2A-GFP
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33544854
Proper citation: RRID:Addgene_162511 Copy
Species: Homo sapiens
Genetic Insert: Tac
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4701; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32826314
Proper citation: RRID:Addgene_162494 Copy
Species: Mus musculus
Genetic Insert: sgRNA targeting Axin1
Vector Backbone Description: Backbone Marker:Feng Zhang lab (Addgene plasmid # 42230); Backbone Size:8500; Vector Backbone:px330; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32814112
Proper citation: RRID:Addgene_162543 Copy
Species: Aequorea victoria
Genetic Insert: mVenus
Vector Backbone Description: Vector Backbone:pC2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33415684
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.10.08.330894v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_162613 Copy
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