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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pFLAG R106L-CPTP Resource Report Resource Website |
RRID:Addgene_170744 | CPTP | Homo sapiens | Ampicillin | PMID:23863933 | Vector Backbone:pFLAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | R106L | 2026-08-15 01:10:11 | 0 | |
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pFLAG CPTP Resource Report Resource Website |
RRID:Addgene_170742 | CPTP | Homo sapiens | Ampicillin | PMID:21625605 | Vector Backbone:pFLAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:10 | 0 | ||
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pFLAG GLTP Resource Report Resource Website |
RRID:Addgene_170740 | GLTP | Homo sapiens | Ampicillin | PMID:21625605 | Vector Backbone:pFLAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:10 | 0 | ||
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ZE31 Prickle-1-pCS2P+ Resource Report Resource Website |
RRID:Addgene_17072 | Prickle-1 | Danio rerio | Ampicillin | PMID:12699626 | Description: Zebrafish prickle-1 (full-length ORF), cloned into the BamH1 and EcoR1 sites of CS2P+. Species of Sequence Origin: zebrafish. Notes for Use: Has internal Not1, Kp1 sites, so linearize with BssH11 and transcribe SP6 for sense RNA. Select clones with amp. Reference: MT Veeman et al., Current Biology 13: 680-685 (2003). | Backbone Size:4100; Vector Backbone:CS2P+; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:10 | 0 | |
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ZE33 Prickle-1-GFPnopren-pCS2+ Resource Report Resource Website |
RRID:Addgene_17074 | Prickle-1 | Danio rerio | Ampicillin | PMID:12699626 | Description: Zebrafish prickle-1, cloned into the vector CS2+GFP-BB, to provide an N-terminal GFP. The C-terminal prenylation motif KNCIIS was deleted by PCR. Species of Sequence Origin: zebrafish. Notes for Use: Has internal Not1, Kp1 sites, so linearize with BssH11 and transcribe SP6 for sense RNA. Select clones with amp. Reference: MT Veeman et al., Current Biology 13: 680-685 (2003). | Backbone Size:0; Vector Backbone:CS2+GFP-BB; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin | Pk1GFPnopren deletes the C-terminal six amino acids containing the putative prenylation site. | 2026-08-15 01:10:10 | 0 |
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ZE22 vox-CS (ZV100) Resource Report Resource Website |
RRID:Addgene_17064 | vox-2 | Danio rerio | Ampicillin | Zvox-2 coding region amplified by PCR and inserted into CS2+ (first cloned into SK as zV93). For RNA: cut with Asp718 and transcribe with SP6. Plasmid History: Species of Sequence Origin: Zebrafish. Constructed by: David Kimelman, 6/28/97 XIX p. 67. | Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:10 | 0 | ||
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ZE26 Stbm-N-6myc-pCS2+ Resource Report Resource Website |
RRID:Addgene_17068 | Stbm | Danio rerio | Ampicillin | PMID:11780127 | 6-c-myc epitopes in frame at the amino terminus of wild type zebrafish strabismus; EcoRI-Xba1 insert. For Sense RNA: linearize with ASP 718 and transcribe with SP6 polymerase. Plasmid History: Species of Sequence Origin: Zebrafish. Reference: M. Park and R. Moon, Nature Cell Biology 4, 20-25, 2002. | Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:10 | 0 | |
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pW212-lenti-spsgRNA-Esp3I-2kb-filler-pEF1s-NLS-mScarlet-I-P2A-BlastR Resource Report Resource Website |
RRID:Addgene_170810 | Ampicillin | PMID:34133940 | 3rd generation lentiviral backbone. Please visit https://www.biorxiv.org/content/10.1101/2020.06.24.165795v2.full for bioRxiv preprint. | Vector Backbone:lentiCRISPR v2 (Addgene #52961); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | NA | 2026-08-15 01:10:10 | 0 | ||
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pFUGW-FIRE-pHLy Resource Report Resource Website 1+ mentions |
RRID:Addgene_170774 | LAMP1 | Homo sapiens | Ampicillin | PMID:34102054 | Chin et al. ACS Sens. 2021 Jun 8. doi: 10.1021/acssensors.0c02318. Pubmed: https://pubmed.ncbi.nlm.nih.gov/34102054/ Article: https://pubs.acs.org/doi/10.1021/acssensors.0c02318 | Backbone Marker:Addgene (Plasmid #14883); Backbone Size:9955; Vector Backbone:FUGW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | 84 nucleotide Signal Sequence switched to the beginning of the construct | 2026-08-15 01:10:11 | 5 |
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pLJM1-FIRE-pHLy Resource Report Resource Website 1+ mentions |
RRID:Addgene_170775 | LAMP1 | Homo sapiens | Ampicillin | PMID:34102054 | Chin et al. ACS Sens. 2021 Jun 8. doi: 10.1021/acssensors.0c02318. Pubmed: https://pubmed.ncbi.nlm.nih.gov/34102054/ Article: https://pubs.acs.org/doi/10.1021/acssensors.0c02318 | Backbone Marker:Addgene Plasmid #19319; Backbone Size:8083; Vector Backbone:pLJM1-EGFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | 84 nucleotide Signal Sequence switched to the beginning of the construct | 2026-08-15 01:10:10 | 4 |
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lacI-Ptac-lacO-BsaI-BsaI-IMBB4-pUC Resource Report Resource Website |
RRID:Addgene_170772 | lacI | Synthetic | Ampicillin | PMID:35255734 | This plasmid was designed to test Golden Gate assembly protocols | Backbone Marker:pTwist; Backbone Size:3; Vector Backbone:pUC; Vector Types:; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:10 | 0 | |
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AICSDP-116:PCNA-mEGFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_170818 | PCNA Homology Arms with mEGFP-linker | Homo sapiens | Ampicillin | This plasmid has been used with locus-specific CRISPR/Cas9 to add a mEGFP tag to the N-terminus of human PCNA in WTC-11 human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: GEGQGQGQGPGRGYAYRS. After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ . | Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin | homology arms contain point mutations to disrupt crRNA binding sites used, and WTC-11 specific homozygous SNP(s) | 2026-08-15 01:10:11 | 1 | |
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pW188-lenti-spsgRNA-lacZ-pEF1s-NLS-mScarlet-I-P2A-BlastR Resource Report Resource Website |
RRID:Addgene_170813 | NLS-mScarlet-I-P2A-BlastR | Synthetic; B. coriaceae | Ampicillin | PMID:34133940 | 3rd generation lentiviral backbone. Please visit https://www.biorxiv.org/content/10.1101/2020.06.24.165795v2.full for bioRxiv preprint. | Vector Backbone:pW212 (Addgene #170810); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | NA | 2026-08-15 01:10:11 | 0 |
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lacI-Ptac-lacO-IMBB4-pUC Resource Report Resource Website |
RRID:Addgene_170770 | lacI | Synthetic | Ampicillin | PMID:35255734 | This plasmid was designed to test 5RM assembly protocols | Backbone Marker:GenScript; Backbone Size:1835; Vector Backbone:pUC; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:11 | 0 | |
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pW210-pPB-GA-inducible-CRISPRa-sa_dCas9-VPR-hygR Resource Report Resource Website |
RRID:Addgene_170808 | GID1-VPR-IRES-HygR | Arabidopsis thaliana | Ampicillin | PMID:34133940 | Please visit https://www.biorxiv.org/content/10.1101/2020.06.24.165795v2.full for bioRxiv preprint. | Vector Backbone:pSLQ2842 (Addgene #84244); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Synonymous mutation in PYL1 to remove XhoI site (inherited from pSLQ2842; Addgene #84244) | 2026-08-15 01:10:10 | 0 |
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pW211-lenti-spsgRNA-Esp3I-2kb-filler-pEF1s-NLS-mNeonGreen-P2A-BlastR Resource Report Resource Website |
RRID:Addgene_170809 | Ampicillin | PMID:34133940 | 3rd generation lentiviral backbone. Please visit https://www.biorxiv.org/content/10.1101/2020.06.24.165795v2.full for bioRxiv preprint. | Vector Backbone:lentiCRISPR v2 (Addgene #52961); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | NA | 2026-08-15 01:10:11 | 0 | ||
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pUASTattB-GFP-Msp300KASH Resource Report Resource Website 1+ mentions |
RRID:Addgene_170806 | GFP-Msp300KASH | Drosophila melanogaster | Ampicillin | PMID:34022041 | Plasmid derived from GFP-Msp300KASH fusion provided by Janice Fischer and described in: Yu, J. et al. (2006) The KASH domain protein MSP-300 plays an essential role in nuclear anchoring during Drosophila oogenesis. Developmental biology 289, 336-345, doi:10.1016/j.ydbio.2005.10.027 Plasmid first described in: Ma, J. and Weake, V.M. (2014). Affinity-based isolation of tagged nuclei from Drosophila tissues for gene expression analysis. Journal of Visualized Experiments 85. PMID:24686501. | Backbone Size:9360; Vector Backbone:pUASTattB; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:11 | 1 | |
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pUASTattB-2xFlag-mCherry-Msp300KASH Resource Report Resource Website |
RRID:Addgene_170807 | 2xFLAG/mCherry-Msp300KASH | Drosophila melanogaster | Ampicillin | PMID:34022041 | Plasmid first described in: Hall H., Medina P., Cooper D.A., Escobedo S.E., Rounds J., Brennan K.J., Vincent C., Miura P., Doerge R. and Weake V.M. (2017). Transcriptome profiling of aging Drosophila photoreceptors reveals gene expression trends that correlate with visual senescence. BMC Genomics. 18(1):894. PMID: 29162050. | Backbone Size:9355; Vector Backbone:pUASTattB; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:10 | 0 | |
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pStr-KDEL_APV-SBP-Dendra2-ER-5 Resource Report Resource Website |
RRID:Addgene_170679 | APV-SBP-Dendra2-ER-5 | Synthetic | Ampicillin | PMID:35051356 | Backbone Marker:Franck Perez Lab (Addgene #65286); Backbone Size:4407; Vector Backbone:Str-KDEL_SBP-EGFP-Ecadherin; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:10 | 0 | ||
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pStr-KDEL_APV-SBP-DsRed2-ER-5 Resource Report Resource Website |
RRID:Addgene_170675 | APV-SBP-DsRed2-ER-5 | Synthetic | Ampicillin | PMID:35051356 | Please see the Genbank file under Resource Information for an annotated sequence file from the depositing laboratory. | Backbone Marker:Franck Perez Lab (Addgene #65286); Backbone Size:4407; Vector Backbone:Str-KDEL_SBP-EGFP-Ecadherin; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:10 | 0 |
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