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Species: Homo sapiens
Genetic Insert: NEDD4L W576A
Vector Backbone Description: Backbone Size:5842; Vector Backbone:pCI-FLAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34284061
Proper citation: RRID:Addgene_166710 Copy
Species: Homo sapiens
Genetic Insert: AAVR-FLAG
Vector Backbone Description: Backbone Marker:addgene plasmid #17452; Backbone Size:9628; Vector Backbone:pLenti-CMV-Puro-DEST; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26814968
Proper citation: RRID:Addgene_166716 Copy
Species: Homo sapiens
Genetic Insert: Angiomotin p130 L106P
Vector Backbone Description: Backbone Size:5446; Vector Backbone:pcDNA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34284061
Proper citation: RRID:Addgene_166714 Copy
Species: Homo sapiens
Genetic Insert: DPP9
Vector Backbone Description: Vector Backbone:pFastBac HTB; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33731932
Comments: Insect cell expression vector for DPP9S (protein purification)
Proper citation: RRID:Addgene_166787 Copy
Species: Homo sapiens
Genetic Insert: GGamma12-GFP2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5432; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32367019
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community.
Proper citation: RRID:Addgene_166781 Copy
Species: Homo sapiens
Genetic Insert: AMOT PPxY1 fused to NEDD4L WW3
Vector Backbone Description: Backbone Size:5597; Vector Backbone:pCA528; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34284061
Proper citation: RRID:Addgene_166705 Copy
Species: Homo sapiens
Genetic Insert: NEDD4L WW2 domain
Vector Backbone Description: Backbone Size:5797; Vector Backbone:pGEX-PP-GFP; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34284061
Proper citation: RRID:Addgene_166702 Copy
Species: Homo sapiens
Genetic Insert: NEDD4L W221A
Vector Backbone Description: Backbone Size:5842; Vector Backbone:pCI-FLAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34284061
Proper citation: RRID:Addgene_166707 Copy
Species: Homo sapiens
Genetic Insert: Bik
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35442739
Comments: Transfection of this plasmid will express "VBik" (Venus fused to the N-terminus of the BH3-only protein, Bik) in live cells. There is an 8 amino acid flexible linker between Venus and Bik proteins. When compared with EYFP, Venus fluorophore contains the following mutations: F46L, F64L, M153T, V163A and S175G. This Venus protein contains an F224R mutation to make it monomeric.
Proper citation: RRID:Addgene_166737 Copy
Species: Homo sapiens
Genetic Insert: Puma
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35442739
Comments: Transfection of this plasmid will express "VPuma" (Venus fused to the N-terminus of the BH3-only protein, Puma) in live cells. When compared with EYFP, Venus fluorophore contains the following mutations: F46L, F64L, M153T, V163A and S175G. This Venus protein contains an F224R mutation to make it monomeric. In cloning, the last amino acid of Venus protein was deleted (K239S). Result is a 1 amino acid linker between Venus and Puma proteins. The encoded BBC3 isoform 4 is also known as PUMA-alpha.
Proper citation: RRID:Addgene_166739 Copy
Species: Homo sapiens
Genetic Insert: Mcl-1
Vector Backbone Description: Vector Backbone:pLVX; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35442739
Comments: 2nd generation lentiviral transfer plasmid that can be used to create lentivirus, for CMV driven expression of mCerulean3 fused to the N-terminus of Mcl-1. There is a flexible, 17 amino acid linker between mCerulean3 and Mcl-1. proteins.
Proper citation: RRID:Addgene_166752 Copy
Species: Homo sapiens
Genetic Insert: Bad-4E
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30860026
Comments: Transfection of this plasmid will express "VBad-4E" (Venus fused to the N-terminus of the BH3-only protein, Bad-4E) in live cells. There is a flexible, 7 amino acid linker between Venus and Bad-4E proteins. Full length Bad protein with BH3 region hydrophobic positions H1-H4 mutated to glutamic acid (E), refered to as "BH3-4E" mutation, BH3 mutations: Y110E, L114E, M117E, F122E. When compared with EYFP, Venus fluorophore contains the following mutations: F46L, F64L, M153T, V163A and S175G. This Venus protein contains an F224R mutation to make it monomeric.
Proper citation: RRID:Addgene_166740 Copy
Species: Homo sapiens
Genetic Insert: Cdc42 T17N
Vector Backbone Description: Backbone Marker:Available at Addgene (#15030); Backbone Size:3300; Vector Backbone:pSP64T; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10862746
Comments: This cDNA encodes a dominant negative version of Cdc42, a Rho GTPase.
The vector is pSP64T, which allows in vitro transcription from the SP6 promoter.
Proper citation: RRID:Addgene_16672 Copy
Species: Homo sapiens
Genetic Insert: DOP1B
Vector Backbone Description: Backbone Marker:Invitrogen (V36620); Backbone Size:5983; Vector Backbone:pcDNA6.2/EmGFP-Bsd/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36995257
Comments: DOP1B (BC156374.1) expressed under the control of CMV promoter and EmGFP-Bsd under the control of PGK
Proper citation: RRID:Addgene_176979 Copy
Species: Homo sapiens
Genetic Insert: PTPalpha
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5178; Vector Backbone:pTet-splice; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10698938
Comments: PTPalpha-HA was amplified from plasmid pXJ41PTPalpha using the 5' primer 5'-CGCCAAGCTTGGCCACCATGGATTCCTGGTTCATTCTTGT-3' and the 3' primer 5'-TGTCAAGCTTCATGCGTAGTCTGCACGTCGTATGGGTACTTGAAGT- TGGCATAATCTGA-3'. The 5' primer contains a HindIII site, a Kozak consensus sequence CCACC and the start codon. The 3' primer contains the coding sequence for the HA epitope YPYDVPDYA, the stop codon and a HindIII site. HindIII-cleaved PCR products were ligated into pTet-Splice. The plasmid contains a CMV promoter under control of regulatory sequences from the tet operon such that, when cotransfected with the transactivator pTet-tTAk (Life Technologies), it expresses protein only in the absence of doxycycline.
Proper citation: RRID:Addgene_17691 Copy
Species: Homo sapiens
Genetic Insert: PTPalpha CCSS
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5178; Vector Backbone:pTet-splice; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10698938
Comments: The plasmid contains a CMV promoter under control of regulatory sequences from the tet operon such that, when cotransfected with the transactivator pTet-tTAk, it expresses protein only in the absence of doxycycline.
Proper citation: RRID:Addgene_17692 Copy
Species: Homo sapiens
Genetic Insert: PTPalpha Y789F
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5178; Vector Backbone:pTet-splice; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10698938
Comments: PTPalpha(Y789F)-HA was generated using pXJ41PTPalpha as a template with the 5' primer 5'-CGCCAAGCTTGGCCACCATGGATTCCTGGTTCATTCTTGT-3' and the 3' primer 5'-TGTCAAGCTTCATGCGTAGTCTGGCACGTCGTATGGGTACTTGAAGTTGGCAAAATCTGA-3'. The substitution ATA->AAA results in a Tyr789->Phe substitution. HindIII-cleaved PCR products were ligated into pTet-Splice. The plasmid contains a CMV promoter under control of regulatory sequences from the tet operon such that, when cotransfected with the transactivator pTet-tTAk, it expresses protein only in the absence of doxycycline.
Proper citation: RRID:Addgene_17693 Copy
Species: Homo sapiens
Genetic Insert: PTPalpha S204A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5178; Vector Backbone:pTet-splice; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11923305
Comments: See article for cloning details. The plasmid contains a CMV promoter under control of regulatory sequences from the tet operon such that, when cotransfected with the transactivator pTet-tTAk, it expresses protein only in the absence of doxycycline.
Proper citation: RRID:Addgene_17694 Copy
Species: Homo sapiens
Genetic Insert: MOSMO
Vector Backbone Description: Backbone Marker:Invitrogen (V36620); Backbone Size:5983; Vector Backbone:pcDNA6.2/EmGFP-Bsd/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36995257
Comments: MOSMO (NM_001164579.2) expressed under the control of CMV promoter and EmGFP-Bsd under the control of PGK
Proper citation: RRID:Addgene_176975 Copy
Species: Homo sapiens
Genetic Insert: RUNX1
Vector Backbone Description: Backbone Marker:Invitrogen (V36620); Backbone Size:5983; Vector Backbone:pcDNA6.2/EmGFP-Bsd/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36995257
Comments: RUNX1 (BC136381) expressed under the control of CMV promoter and EmGFP-Bsd under the control of PGK
Proper citation: RRID:Addgene_176974 Copy
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