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Species: Mus musculus
Genetic Insert: Tbx21
Vector Backbone Description: Vector Backbone:gRNA backbone; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34161760
Proper citation: RRID:Addgene_170838 Copy
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pCS2+ (w/ GFP); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Linearize with Not1 or Asp718 and transcribe with SP6.
Notes for Use:
For making carboxy-terminal tagged GFP fusion protein in vector CS2+.
Made by: Jeff Miller.
Proper citation: RRID:Addgene_17097 Copy
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pCS2+ (w/ GFP); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Linearize with Not1 or Asp718 and transcribe with SP6.
Notes for Use:
For making amino-terminal GFP fusion protein in vector CS2+.
Made by: Jeff Miller.
Proper citation: RRID:Addgene_17099 Copy
Species: Mus musculus
Genetic Insert: caveolae associated 1 (Cavin1)
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34172739
Proper citation: RRID:Addgene_170859 Copy
Species: S. japonia/camelidae/Staphylococcus aureus
Genetic Insert: Glutathione-S-Transferase/LaG16/MNase
Vector Backbone Description: Backbone Marker:GE Healthcare Life Sciences; Backbone Size:4304; Vector Backbone:pGEX-2T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34644460
Proper citation: RRID:Addgene_170978 Copy
Vector Backbone Description: Backbone Size:4103; Vector Backbone:pCS2+ (modified); Vector Types:xenopus/mammalian/avian/zebrafish; Bacterial Resistance:Ampicillin
Comments: pCS2+ has been modified by the deletion of the AC preceeding the T7 promoter addition of the sequence TTCTGCAGCCC immediately following the XbaI site. The PstI site added to CS2+ is encompassed in the sequence (CTGCAG).
Modifications were made by Robert Davis of Marc Kirshner's lab to improve the efficiency of the transcription from the T7 promoter.
Proper citation: RRID:Addgene_17095 Copy
Vector Backbone Description: Backbone Size:5090; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 2005. Fire Lab Miniprep Number pPD158.87, Ligation number L5767.
Proper citation: RRID:Addgene_1709 Copy
Species: Danio rerio
Genetic Insert: Dvl-2
Vector Backbone Description: Backbone Size:4100; Vector Backbone:CS2P+; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15539487
Comments: Dishevelled 2 deleted in amino acids 231-252, cloned into Cla/EcoR1 sites.
Notes for Use: For functional synthetic RNA cut ASP718, transcribe SP6.
Select clones with amp.
Reference: Waxman JS, Hocking AM, Stoick CL, Moon RT.
Zebrafish Dapper1 and Dapper2 play distinct roles in Wnt-mediated developmental processes.
Development. 2004 Dec;131(23):5909-21.
Proper citation: RRID:Addgene_17087 Copy
Species: Danio rerio
Genetic Insert: Dvl-3
Vector Backbone Description: Backbone Size:4100; Vector Backbone:CS2P+; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15539487
Comments: Dishevelled 3, cloned into Cla/EcoR1 sites.
Notes for Use: For functional synthetic RNA cut NotI, transcribe SP6.
Select clones with amp.
Reference: Waxman JS, Hocking AM, Stoick CL, Moon RT.
Zebrafish Dapper1 and Dapper2 play distinct roles in Wnt-mediated developmental processes.
Development. 2004 Dec;131(23):5909-21.
Proper citation: RRID:Addgene_17089 Copy
Species: Synthetic
Genetic Insert: EGFP-uTEV1-MAVS(tmd)[R537A]
Vector Backbone Description: Backbone Size:8000; Vector Backbone:pCW-Cas9; Vector Types:Mammalian Expression, Lentiviral, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34414886
Proper citation: RRID:Addgene_170999 Copy
Species: Homo sapiens
Genetic Insert: RE8
Vector Backbone Description: Vector Backbone:pRF TpC; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33852895
Proper citation: RRID:Addgene_171047 Copy
Species: Homo sapiens
Genetic Insert: RE7
Vector Backbone Description: Vector Backbone:pRF TpC; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33852895
Proper citation: RRID:Addgene_171046 Copy
Species: Homo sapiens
Genetic Insert: RE5
Vector Backbone Description: Vector Backbone:pRF TpC; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33852895
Proper citation: RRID:Addgene_171044 Copy
Species: Homo sapiens
Genetic Insert: RE4
Vector Backbone Description: Vector Backbone:pRF TpC; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33852895
Proper citation: RRID:Addgene_171043 Copy
Species: Homo sapiens
Genetic Insert: RE2
Vector Backbone Description: Vector Backbone:pRF TpC; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33852895
Proper citation: RRID:Addgene_171041 Copy
Species: Homo sapiens
Genetic Insert: RE1
Vector Backbone Description: Vector Backbone:pRF TpC; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33852895
Proper citation: RRID:Addgene_171040 Copy
Vector Backbone Description: Vector Backbone:pRF; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33852895
Proper citation: RRID:Addgene_171039 Copy
Species: Homo sapiens
Genetic Insert: Fyn
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34161759
Proper citation: RRID:Addgene_171035 Copy
Species: Homo sapiens
Genetic Insert: LAT
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34161759
Proper citation: RRID:Addgene_171032 Copy
Species: Homo sapiens
Genetic Insert: EZH2
Vector Backbone Description: Vector Backbone:pX330; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32632336
Proper citation: RRID:Addgene_171031 Copy
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