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Species: Ideonella sakaiensis 201-F6
Genetic Insert: PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1)
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5367; Vector Backbone:pET-21b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32989159
Proper citation: RRID:Addgene_162675 Copy
Species: Ideonella sakaiensis 201-F6
Genetic Insert: MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5367; Vector Backbone:pET-21b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32989159
Proper citation: RRID:Addgene_162676 Copy
Species: Mus musculus
Genetic Insert: HB9 promoter
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:Mammalian Expression, Mouse Targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15958737
Proper citation: RRID:Addgene_16283 Copy
Vector Backbone Description: Backbone Size:10236; Vector Backbone:pTIN7m; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32788323
Proper citation: RRID:Addgene_162886 Copy
Species: Synthetic
Genetic Insert: 2_6_RNA-device
Vector Backbone Description: Backbone Marker:https://doi.org/10.1093/nar/gks636; Backbone Size:8187; Vector Backbone:pCS1748; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33915105
Proper citation: RRID:Addgene_162800 Copy
Species: Homo sapiens
Genetic Insert: human Glucose response protein 78 promoter
Vector Backbone Description: Vector Backbone:pDonr231; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24395366
Proper citation: RRID:Addgene_162970 Copy
Species: Synthetic
Genetic Insert: optimized human CMV promoter
Vector Backbone Description: Vector Backbone:pDonr215; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24395366
Proper citation: RRID:Addgene_162973 Copy
Species: Homo sapiens
Genetic Insert: human EF1alpha promoter
Vector Backbone Description: Vector Backbone:pDonr215; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24395366
Proper citation: RRID:Addgene_162975 Copy
Species: Homo sapiens
Genetic Insert: methyltransferase like 3
Vector Backbone Description: Backbone Size:8762; Vector Backbone:Tet-pLKO-puro; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34088870
Proper citation: RRID:Addgene_162984 Copy
Species: Synthetic
Genetic Insert: exchangeable cassette
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Mammalian Expression, Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34732717
Comments: sgRNA targets can be cloned between BpiI sites
Proper citation: RRID:Addgene_162987 Copy
Species: Synthetic
Genetic Insert: exchangeable cassette
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Mammalian Expression, Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34732717
Comments: sgRNA targets can be cloned between BpiI sites
Proper citation: RRID:Addgene_162988 Copy
Species: Xenopus laevis
Genetic Insert: Axin
Vector Backbone Description: Backbone Size:4352; Vector Backbone:pCS2+MT; Vector Types:Vertebrate expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10072781
Comments: For sense mRNA, linearize with NotI and transcribe with SP6.
For antisense probe, cut with Pst1, remove 3' overhang, and transcribe with T7.
NcoI digest cuts out a 2333 bp fragment.
Proper citation: RRID:Addgene_16298 Copy
Species: Homo sapiens
Genetic Insert: p53 promoter fragment
Vector Backbone Description: Backbone Marker:promega; Backbone Size:5598; Vector Backbone:pGL2 basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16849542
Proper citation: RRID:Addgene_16295 Copy
Species: Homo sapiens
Genetic Insert: p53 promoter fragment
Vector Backbone Description: Backbone Marker:promega; Backbone Size:5598; Vector Backbone:pGL2 basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16849542
Proper citation: RRID:Addgene_16294 Copy
Species: Synthetic
Genetic Insert: optimized CMV promoter
Vector Backbone Description: Vector Backbone:pDonr233; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24395366
Proper citation: RRID:Addgene_162927 Copy
Species: Homo sapiens
Genetic Insert: human Ubiquitin C promoter
Vector Backbone Description: Vector Backbone:pDonr233; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24395366
Proper citation: RRID:Addgene_162925 Copy
Species: Zoanthus sp.
Genetic Insert: Zs Green
Vector Backbone Description: Backbone Marker:Cell Biolabs; Backbone Size:4000; Vector Backbone:AAV2; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33024795
Proper citation: RRID:Addgene_163020 Copy
Species: S. aureus
Genetic Insert: Mammalian codon-optimized SadCas9
Vector Backbone Description: Backbone Marker:Cell Biolabs; Backbone Size:3500; Vector Backbone:AAV2; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33024795
Proper citation: RRID:Addgene_163024 Copy
Species: Caenorhabditis elegans
Genetic Insert: 30aa linker::mNeonGreen (dpi)::AID*::3xFLAG::10aa linker
Vector Backbone Description: Backbone Size:2360; Vector Backbone:pDONR221; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33677541
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.05.12.090217v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_163095 Copy
Species: Synthetic
Genetic Insert: Nrxn3b-MKII-f-hLOV1-TEVcs-tTA
Vector Backbone Description: Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33308478
Proper citation: RRID:Addgene_163031 Copy
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