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Species: N meningitidis
Genetic Insert: NmCas9
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pSimpleII; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23940360
Proper citation: RRID:Addgene_47868 Copy
Species: Synthetic
Genetic Insert: tdTomato
Vector Backbone Description: Backbone Marker:Martin P. Vazquez; Backbone Size:6200; Vector Backbone:pTrex-Neo; Vector Types:Trypanasoma cruzi expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20644616
Comments: Shaner NC, Campbell RE, Steinbach PA, Giepmans BN, Palmer AE, Tsien RY. Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nat Biotechnol. 2004;22:1567–1572
Vazquez MP, Levin MJ (1999) Functional analysis of the intergenic regions of TcP2beta gene loci allowed the construction of an improved Trypanosoma cruzi expression vector. Gene 239: 217–225. doi: 10.1016/S0378-1119(99)00386-8.
Proper citation: RRID:Addgene_47975 Copy
Vector Backbone Description: Backbone Marker:self-made; Backbone Size:8482; Vector Backbone:two ori derived from pUC19 and pPZP201; Vector Types:Unspecified; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21364738
Comments: level 2 vector for cloning directly from level 0
Proper citation: RRID:Addgene_48076 Copy
Vector Backbone Description: Backbone Marker:self-made; Backbone Size:4349; Vector Backbone:derived from pBIN19 and pUC19; Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21364738
Comments: level 2 dummy fragment
Proper citation: RRID:Addgene_48068 Copy
Vector Backbone Description: Backbone Marker:self-made; Backbone Size:4349; Vector Backbone:derived from pBIN19 and pUC19; Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21364738
Comments: level 2 dummy fragment
Proper citation: RRID:Addgene_48067 Copy
Vector Backbone Description: Backbone Marker:self-made; Backbone Size:2247; Vector Backbone:pUC19-derived; Vector Types:Unspecified; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:21364738
Comments: level 0 cloning vector
Proper citation: RRID:Addgene_47996 Copy
Vector Backbone Description: Backbone Marker:self-made; Backbone Size:2247; Vector Backbone:pUC19-derived; Vector Types:Unspecified; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:21364738
Comments: level 0 cloning vector
Proper citation: RRID:Addgene_47994 Copy
Vector Backbone Description: Backbone Marker:self-made; Backbone Size:2247; Vector Backbone:pUC19-derived; Vector Types:Unspecified; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:21364738
Comments: level 0 cloning vector
Proper citation: RRID:Addgene_47997 Copy
Vector Backbone Description: Backbone Marker:self-made; Backbone Size:2247; Vector Backbone:pUC19-derived; Vector Types:Unspecified; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:21364738
Comments: level 0 cloning vector
Proper citation: RRID:Addgene_47998 Copy
Vector Backbone Description: Backbone Marker:self-made; Backbone Size:4604; Vector Backbone:derived from pBIN19 and pUC19; Vector Types:Unspecified; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:21364738
Comments: level M cloning vector, replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery to plant cells
Proper citation: RRID:Addgene_48043 Copy
Vector Backbone Description: Backbone Marker:self-made; Backbone Size:3137; Vector Backbone:pUC19-derived; Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21364738
Comments: level M end linker
Proper citation: RRID:Addgene_48046 Copy
Vector Backbone Description: Backbone Marker:self-made; Backbone Size:2247; Vector Backbone:pUC19-derived; Vector Types:Unspecified; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:21364738
Comments: level 0 cloning vector
Proper citation: RRID:Addgene_47992 Copy
Vector Backbone Description: Backbone Marker:self-made; Backbone Size:2247; Vector Backbone:pUC19-derived; Vector Types:Unspecified; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:21364738
Comments: level 0 cloning vector
Proper citation: RRID:Addgene_47985 Copy
Vector Backbone Description: Backbone Marker:self-made; Backbone Size:4824; Vector Backbone:pAGM4351; Vector Types:Unspecified; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21364738
Comments: PLEASE NOTE- It has recently come to light that plasmid pAGM4723 lacks a complete overdrive sequence at the right border. This can cause incomplete TDNA insertion events and can give a higher incidence of vector backbone transfer events due to transfer initiating at the left border.
Level 2 cloning vector, replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery to plant cells.
Please note that bacterial colonies for this plasmid often appear red in color because the plasmid contains a red color selectable marker (CRed--an artificial bacterial operon responsible for canthaxanthin biosynthesis).
Proper citation: RRID:Addgene_48015 Copy
Vector Backbone Description: Backbone Marker:self-made; Backbone Size:3293; Vector Backbone:pUC19-derived; Vector Types:Unspecified; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:21364738
Comments: level 2 end linker
Proper citation: RRID:Addgene_48019 Copy
Vector Backbone Description: Backbone Marker:self-made; Backbone Size:4356; Vector Backbone:derived from pBIN19 and pUC19; Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21364738
Comments: level 1 cloning vector, replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery to plant cells
Proper citation: RRID:Addgene_48007 Copy
Vector Backbone Description: Backbone Marker:self-made; Backbone Size:4356; Vector Backbone:derived from pBIN19 and pUC19; Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21364738
Comments: level 1 cloning vector, replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery to plant cells
Proper citation: RRID:Addgene_48004 Copy
Vector Backbone Description: Backbone Marker:self-made; Backbone Size:4356; Vector Backbone:derived from pBIN19 and pUC19; Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21364738
Comments: level 1 cloning vector, replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery to plant cells
Proper citation: RRID:Addgene_48005 Copy
Vector Backbone Description: Backbone Marker:self-made; Backbone Size:4356; Vector Backbone:derived from pBIN19 and pUC19; Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21364738
Comments: level 1 cloning vector, replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery to plant cells
Proper citation: RRID:Addgene_48009 Copy
Vector Backbone Description: Backbone Marker:self-made; Backbone Size:4356; Vector Backbone:derived from pBIN19 and pUC19; Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21364738
Comments: level 1 cloning vector, replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery to plant cells
Proper citation: RRID:Addgene_48000 Copy
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