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Species: Homo sapiens
Genetic Insert: NGFR:LMP1
Vector Backbone Description: Backbone Marker:Addgene plasmid #36972; Vector Backbone:pCMMP-MCS-IRES-mRFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22318153
Comments: Sugden lab plasmid p3856
The NGFR-LMP1 fusion in the plasmid contains the amino terminus of NGFR fused to the carboxy terminus of LMP1. The carboxy-terminal signaling of LMP1 is conditionally activated when anti-NGFR antibody crosslinks with the NGFR
moieties. Please see associated publication (link below) for more details.
Proper citation: RRID:Addgene_36974 Copy
Species: Homo sapiens
Genetic Insert: MOB1B
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5472; Vector Backbone:pCl-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18362890
Proper citation: RRID:Addgene_37024 Copy
Species: Homo sapiens
Genetic Insert: CUL3
Vector Backbone Description: Backbone Marker:Moon Lab Plasmid available from Addgene (plasmid #15100); Backbone Size:5500; Vector Backbone:pGLUE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22215675
Comments: Learn more about the pGLUE tandem-affinity purification plasmid backbone at http://www.addgene.org/15100/ .
Proper citation: RRID:Addgene_36970 Copy
Species: Homo sapiens
Genetic Insert: RASSF6b
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pCl-neo (modified); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19797269
Proper citation: RRID:Addgene_37021 Copy
Species: Homo sapiens
Genetic Insert: WTX K292N
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6100; Vector Backbone:pcDNA3.1 (with Venus added); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22215675
Proper citation: RRID:Addgene_36966 Copy
Species: Homo sapiens
Genetic Insert: WTX (delta ETGE motif)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6100; Vector Backbone:pcDNA3.1 (with Venus added); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22215675
Proper citation: RRID:Addgene_36967 Copy
Species: Homo sapiens
Genetic Insert: rassf1A
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pCl-neo (modified); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19797269
Comments: pCIneoFH vector has the sequence NheI-FLAG-His6-EcoRI-MluI-XbaI-SalI-SmaI-NotI.
A digest with NheI and SalI will excise the insert with tags.
Proper citation: RRID:Addgene_37016 Copy
Species: Homo sapiens
Genetic Insert: perfect complementary binding site for hsa-miR-34a
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:6273; Vector Backbone:psiCHECK2; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19584398
Proper citation: RRID:Addgene_37099 Copy
Species: Homo sapiens
Genetic Insert: FBXW11
Vector Backbone Description: Backbone Marker:Moon Lab Plasmid available from Addgene (plasmid #15100); Backbone Size:5500; Vector Backbone:pGLUE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22215675
Comments: Learn more about the pGLUE tandem-affinity purification plasmid backbone at http://www.addgene.org/15100/ .
Proper citation: RRID:Addgene_36969 Copy
Species: Homo sapiens
Genetic Insert: GLI1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9452474
Proper citation: RRID:Addgene_37113 Copy
Species: Homo sapiens
Genetic Insert: EDD
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4373; Vector Backbone:pCMV-Tag2B; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12011095
Comments: Addgene NGS results identified a K503R mutation within the UBR5 translation. There is no information on the functional consequence of this mutation
Proper citation: RRID:Addgene_37188 Copy
Species: Homo sapiens
Genetic Insert: EDD
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4708; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12011095
Proper citation: RRID:Addgene_37190 Copy
Species: Homo sapiens
Genetic Insert: EDD C2768A
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4225; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12011095
Comments: The depositing lab has noted that mutation K503R shouldn’t be a problem as it is outside the main functional domains. There does appear to be a relatively common SNP at K503 in Latino populations so this might be a genuine SNP-
http://exac.broadinstitute.org/variant/8-103338864-C-T
https://www.ncbi.nlm.nih.gov/projects/SNP/snp_ref.cgi?rs=rs571861064
Proper citation: RRID:Addgene_37192 Copy
Species: Homo sapiens
Genetic Insert: A2BR
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEYFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21628448
Comments: A2B was PCR amplified as a 1068bp fragment incorporating a 5' EcoRI site. The stop codon was eliminated and a 3' BamHI site was added with the reverse PCR primer to allow direct cloning into pEYFP-N1
Proper citation: RRID:Addgene_37202 Copy
Species: Homo sapiens
Genetic Insert: lig4
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4776; Vector Backbone:pFASTBAC1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_37150 Copy
Species: Homo sapiens
Genetic Insert: XRCC6
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4776; Vector Backbone:pFASTBAC1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_37148 Copy
Species: Homo sapiens
Genetic Insert: XRCC5
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4776; Vector Backbone:pFASTBAC1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_37149 Copy
Species: Homo sapiens
Genetic Insert: vang-like 2
Vector Backbone Description: Backbone Size:4224; Vector Backbone:pAdLox GI 1488684; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16791850
Comments: contains EGFP hVangl2 WT
Proper citation: RRID:Addgene_37257 Copy
Species: Homo sapiens
Genetic Insert: Scribble
Vector Backbone Description: Backbone Size:4224; Vector Backbone:pAdLox GI 1488684; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16791850
Comments: contains EGFP C-term
Proper citation: RRID:Addgene_37256 Copy
Species: Homo sapiens
Genetic Insert: Scribble
Vector Backbone Description: Backbone Size:4224; Vector Backbone:pAdLox GI 1488684; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16791850
Comments: contains EGFP-95-1630 hScrib
Proper citation: RRID:Addgene_37252 Copy
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