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Species: Synthetic
Genetic Insert: GPI anchor
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pIRESneo3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22406856
Comments: luminal tagging
Proper citation: RRID:Addgene_65296 Copy
Species: Synthetic
Genetic Insert: URR2
Vector Backbone Description: Vector Backbone:pMV; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25956650
Proper citation: RRID:Addgene_65332 Copy
Species: Synthetic
Genetic Insert: GPI anchor
Vector Backbone Description: Backbone Marker:system biosciences; Vector Backbone:pCDH-CMV-MCS-EF1-puro; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22406856
Comments: luminal tagging
Proper citation: RRID:Addgene_65298 Copy
Species: Synthetic
Genetic Insert: vioB
Vector Backbone Description: Vector Backbone:pKMV; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25956650
Proper citation: RRID:Addgene_65348 Copy
Species: Synthetic
Genetic Insert: ycrtE
Vector Backbone Description: Vector Backbone:HCKan-O; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25956650
Proper citation: RRID:Addgene_65344 Copy
Species: Synthetic
Genetic Insert: vioA
Vector Backbone Description: Vector Backbone:pKMV; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25956650
Proper citation: RRID:Addgene_65347 Copy
Species: Synthetic
Genetic Insert: ycrtYB
Vector Backbone Description: Vector Backbone:PMV; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25956650
Proper citation: RRID:Addgene_65343 Copy
Species: Synthetic
Genetic Insert: ycrtI
Vector Backbone Description: Vector Backbone:PMV; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25956650
Proper citation: RRID:Addgene_65342 Copy
Species: Synthetic
Genetic Insert: vioE
Vector Backbone Description: Vector Backbone:pKMV; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25956650
Proper citation: RRID:Addgene_65351 Copy
Species: Synthetic
Genetic Insert: Y-GECO1.0
Vector Backbone Description: Backbone Size:4563; Vector Backbone:AAV with hSyn promoter; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24840546
Proper citation: RRID:Addgene_58200 Copy
Species: Synthetic
Genetic Insert: G-CEPIA1er
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4819; Vector Backbone:pCMV/myc/ER; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24923787
Proper citation: RRID:Addgene_58215 Copy
Species: Synthetic
Genetic Insert: CEPIA2mt
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4799; Vector Backbone:pCMV/myc/mito; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24923787
Proper citation: RRID:Addgene_58218 Copy
Species: Synthetic
Genetic Insert: CEPIA4mt
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4799; Vector Backbone:pCMV/myc/mito; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24923787
Proper citation: RRID:Addgene_58220 Copy
Species: Synthetic
Genetic Insert: pea APX W41F
Vector Backbone Description: Backbone Size:2226; Vector Backbone:pZE; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24803433
Comments: This plasmid encodes the W41F mutant of pea ascorbate peroxidase (APX). The W41F mutation improves enzyme activity. APX W41F is a constitutive homodimer, unlike "APEX", so APX W41F should not be used as a fusion to proteins that are sensitive to dimeric tags.
This plasmid encodes APX W41F under a constitutive promoter, and the promoter is flanked by TetR operator sites. When the plasmid is introduced into cells constitutively expressing the TetR protein, such as the GM1655 Pro cells, then the TetR protein binds to the promoter and suppresses transcription. When a tetracycline drug, such as anhydrotetracycline, is added, the TetR protein is displaced from the promoter, allowing transcription of the APX W41F gene. When the plasmid is introduced into bacterial strains that do not encode a copy of the TetR repressor, expression of APX W41F is constitutive. Any bacterial strain can be used for plasmid propagation.
The pZE plasmid system was developed by Lutz and Bujard, and described in their paper entitled "Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements" (PMID: 9092630).
Proper citation: RRID:Addgene_58199 Copy
Species: Synthetic
Genetic Insert: U6.gRNAS1 cassette
Vector Backbone Description: Backbone Size:6930; Vector Backbone:pBR322-derived pAdEasy plasmid; Vector Types:Mammalian Expression, Adenoviral, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24870050
Comments:
Proper citation: RRID:Addgene_58252 Copy
Species: Synthetic
Genetic Insert: U6.gRNAGFP cassette
Vector Backbone Description: Backbone Size:7043; Vector Backbone:pBR322-derived pAdEasy plasmid; Vector Types:Mammalian Expression, Adenoviral, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24870050
Comments:
Proper citation: RRID:Addgene_58255 Copy
Species: Synthetic
Genetic Insert: Genomic cassette
Vector Backbone Description: Vector Backbone:SEVA; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:24847673
Proper citation: RRID:Addgene_58305 Copy
Species: Synthetic
Genetic Insert: Genomic cassette
Vector Backbone Description: Vector Backbone:SEVA; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:24847673
Proper citation: RRID:Addgene_58304 Copy
Species: Synthetic
Genetic Insert: Genomic cassette
Vector Backbone Description: Vector Backbone:SEVA; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:24847673
Proper citation: RRID:Addgene_58307 Copy
Species: Synthetic
Genetic Insert: PTEF-HygMX-TTEF-insul-(lexA-box)2-PminCYC1
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2884; Vector Backbone:pBS; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25034689
Proper citation: RRID:Addgene_58440 Copy
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