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Species: Gallus gallus
Genetic Insert: goosecoid
Vector Backbone Description: Backbone Size:11600; Vector Backbone:RCASBP(A); Vector Types:Retroviral, avian expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9486529
Comments: gsc ORF ligated into NcoI-Sal I of the Cla 12 Nco adaptor and then cloned into the Cla I site of RCAS (A).
Addgene QC NGS analysis identifies a P2A amino acid discrepancy in the goosecoid insert sequence, relative to NP_990662.2. It is unknown what, if any, impact this may have on plasmid function.
Proper citation: RRID:Addgene_13984 Copy
Species: Gallus gallus
Genetic Insert: Ihh
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBS SK-; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8662546
Comments: Total coding sequence plus 327bp 5’ UTR and 130bp 3’ UTR, from Andrea Vortkamp.
Proper citation: RRID:Addgene_13997 Copy
Species: Gallus gallus
Genetic Insert: Ihh
Vector Backbone Description: Backbone Size:11600; Vector Backbone:RCASBP(A); Vector Types:Retroviral, avian expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8662546
Comments: Start codon (328) to BamHI site of Ihh, from Andrea Vortkamp.
Proper citation: RRID:Addgene_13998 Copy
Species: Gallus gallus
Genetic Insert: HoxA11
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBS SK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Full-length plus 3’UTR chick Hox A11, from Craig Nelson.
Proper citation: RRID:Addgene_14007 Copy
Species: Gallus gallus
Genetic Insert: HoxA9
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBS SK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Full-length plus 3’UTR chick Hox A9, from Craig Nelson.
Proper citation: RRID:Addgene_14008 Copy
Species: Gallus gallus
Genetic Insert: Fgfr2
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBS SK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15253939
Proper citation: RRID:Addgene_14003 Copy
Species: Gallus gallus
Genetic Insert: Fgfr3
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBS SK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15253939
Proper citation: RRID:Addgene_14004 Copy
Species: Gallus gallus
Genetic Insert: HoxB8
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBS SK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Full-length chick Hox B8, from Craig Nelson. Note that there is no exact match for this isoform in GenBank.
Proper citation: RRID:Addgene_14009 Copy
Species: Gallus gallus
Genetic Insert: Wnt11
Vector Backbone Description: Backbone Size:11600; Vector Backbone:RCASBP(A); Vector Types:Retroviral, avian expression; Bacterial Resistance:Ampicillin
Comments: From Yuji Yokouchi.
Proper citation: RRID:Addgene_14018 Copy
Species: Gallus gallus
Genetic Insert: Nodal
Vector Backbone Description: Backbone Size:11600; Vector Backbone:RCASBP(A); Vector Types:Retroviral, avian expression; Bacterial Resistance:Ampicillin
Comments: Mature region of nodal (~400bp) plus the pro region of cBMP 4 (~870bp).
Proper citation: RRID:Addgene_14014 Copy
Species: Gallus gallus
Genetic Insert: HoxD9
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBS SK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Full-length Hox D9, from Jacquie Campbell.
Proper citation: RRID:Addgene_14012 Copy
Species: Gallus gallus
Genetic Insert: HoxD11
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBS SK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Full-length Hox D11, from Craig Nelson.
Proper citation: RRID:Addgene_14011 Copy
Species: Gallus gallus
Genetic Insert: Ltap
Vector Backbone Description: Backbone Size:11600; Vector Backbone:RCASBP(A); Vector Types:Retroviral, avian expression; Bacterial Resistance:Ampicillin
Comments: Chick Ltap full-length ORF, from Kyle Vogan.
Proper citation: RRID:Addgene_14023 Copy
Species: Gallus gallus
Genetic Insert: Chicken Beta 1 Integrin
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:2869; Vector Backbone:pGEM-2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:3487386
Proper citation: RRID:Addgene_14067 Copy
Species: Gallus gallus
Genetic Insert: Chicken Fibronectin
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:2867; Vector Backbone:pGem-3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:2830487
Proper citation: RRID:Addgene_14063 Copy
Species: Gallus gallus
Genetic Insert: Chicken Fibronectin EIIIB
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:2867; Vector Backbone:pGem-3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:2830487
Proper citation: RRID:Addgene_14064 Copy
Species: Gallus gallus
Genetic Insert: Chicken Fibronectin
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:2869; Vector Backbone:pGEM-2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:2830487
Proper citation: RRID:Addgene_14061 Copy
Species: Gallus gallus
Genetic Insert: beta-catenin
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12123611
Comments: *Note that this plasmid--and all of the beta-catenin plasmids associated with this paper--have L95R and S96A mutations relative to the canonical WT. Addgene believes these are polymorphisms and that our stock is representative of what was used in the associated paper. Additionally, we see G636A
Proper citation: RRID:Addgene_16071 Copy
Species: Gallus gallus
Genetic Insert: beta-catenin
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12123611
Comments: Phosphorylation-mimic mutant of beta-catenin.
*Note: In addition to the described mutation, Addgene's quality control sequencing finds the following polymorphisms: L95R, S96A, G636A, and V651G.
Proper citation: RRID:Addgene_16073 Copy
Species: Gallus gallus
Genetic Insert: rASAP
Vector Backbone Description: Vector Backbone:pCDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34620975
Proper citation: RRID:Addgene_178205 Copy
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