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Species: SARS-CoV-2
Genetic Insert: SARS-CoV-2 ORF3a Q57H
Vector Backbone Description: Vector Backbone:pDON221; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34709727
Proper citation: RRID:Addgene_180072 Copy
Species: SARS-CoV-2
Genetic Insert: SARS-CoV-2 NSP5 K90R
Vector Backbone Description: Vector Backbone:pDON223; Vector Types:Mammalian Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34709727
Proper citation: RRID:Addgene_180073 Copy
Species: SARS-CoV-2
Genetic Insert: Spike (omicron BA.1 variant)
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pcDNA3.3; Vector Types:Mammalian Expression, Lentiviral, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35857439
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.04.11.487879v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_180375 Copy
Species: SARS-CoV-2
Genetic Insert: Spike (BA.2)
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pcDNA3.3; Vector Types:Mammalian Expression, Lentiviral, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35857439
Proper citation: RRID:Addgene_183700 Copy
Species: SARS-CoV-2
Genetic Insert: SARS-CoV-2 secretable trimeric Spike
Vector Backbone Description: Backbone Marker:Eric Kowarz; Backbone Size:6625; Vector Backbone:pSBbi-RP (Addgene Plasmid #60513); Vector Types:Mammalian Expression, Transposon; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36619904
Comments: The SARS-CoV-2 secretable trimeric Spike insert was designed by Jorge L. Arias-Arias from Universidad de Costa Rica ([email protected]).
This vector is suitable for transient transfection, but it was intended to be co-transfected with the transposase vector pCMV(CAT)T7-SB100 (Addgene Plasmid #34879) in order to generate stable mammalian cells for the production of SARS-CoV-2 secretable trimeric Spike protein. This protein can be recovered from the cell culture medium by His-tag purification.
Please note that this plasmid is intended to be used as a DNA template of the spike gene but not as a construct for protein production. The protein produced using this plasmid is not antigenically functional.
Proper citation: RRID:Addgene_161794 Copy
Species: SARS-CoV-2
Genetic Insert: nsp8
Vector Backbone Description: Backbone Size:5200; Vector Backbone:pET46; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34041264
Proper citation: RRID:Addgene_164578 Copy
Species: SARS-CoV-2
Genetic Insert: SARS-CoV-2 soluble spike
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32991842
Proper citation: RRID:Addgene_164651 Copy
Species: SARS-CoV-2
Genetic Insert: SARS-CoV-2 soluble spike
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32991842
Proper citation: RRID:Addgene_164652 Copy
Species: SARS-CoV-2
Genetic Insert: CoV-2 frB
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37988285
Comments: detailed explanation of CLEVER reverse genetics methodology in DOI: 10.7554/eLife.89035
Proper citation: RRID:Addgene_253183 Copy
Species: SARS-CoV-2
Genetic Insert: SARS-CoV-2 Omicron XBB.1.5 spike
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37988285
Comments: Detailed explanation of CLEVER reverse genetics methodology in https://doi.org/10.7554/eLife.89035
Proper citation: RRID:Addgene_253119 Copy
Species: SARS-CoV-2
Genetic Insert: SARS-CoV-2 Omicron BA.1 spike
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37988285
Comments: Detailed explanation of CLEVER reverse genetics methodology in https://doi.org/10.7554/eLife.89035
Proper citation: RRID:Addgene_253116 Copy
Species: SARS-CoV-2
Genetic Insert: Cytosolic tail of SARS-CoV-2 spike carrying an N-terminal GST-tag
Vector Backbone Description: Vector Backbone:pGEX5X3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38102143
Proper citation: RRID:Addgene_218455 Copy
Species: SARS-CoV-2
Genetic Insert: Spike (CH.1.1)
Vector Backbone Description: Vector Backbone:pDMJ2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40345199
Proper citation: RRID:Addgene_241112 Copy
Species: SARS-CoV-2
Genetic Insert: Spike (BA.5-2e)
Vector Backbone Description: Vector Backbone:pDMJ2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40345199
Proper citation: RRID:Addgene_241102 Copy
Species: SARS-CoV-2
Genetic Insert: Spike (BA.1)
Vector Backbone Description: Vector Backbone:pDMJ2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40345199
Proper citation: RRID:Addgene_241055 Copy
Species: SARS-CoV-2
Genetic Insert: Nucleocapsid protein
Vector Backbone Description: Backbone Size:5222; Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:39464032
Comments: The depositor confirms that discrepancies between the depositor's sequence and Addgene's sequences have no functional consequences.
Proper citation: RRID:Addgene_242452 Copy
Species: SARS-CoV-2
Genetic Insert: Spike (BA.2.75-4e)
Vector Backbone Description: Vector Backbone:pDMJ2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40345199
Proper citation: RRID:Addgene_241080 Copy
Species: SARS-CoV-2
Genetic Insert: Spike (BA.2.75-5a)
Vector Backbone Description: Vector Backbone:pDMJ2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40345199
Proper citation: RRID:Addgene_241081 Copy
Species: SARS-CoV-2
Genetic Insert: Spike (BA.2.75-5d)
Vector Backbone Description: Vector Backbone:pDMJ2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40345199
Proper citation: RRID:Addgene_241084 Copy
Species: SARS-CoV-2
Genetic Insert: Spike (BA.2.75-6b)
Vector Backbone Description: Vector Backbone:pDMJ2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40345199
Proper citation: RRID:Addgene_241086 Copy
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