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Species: Synthetic
Genetic Insert: Unmarked Linker
Vector Backbone Description: Backbone Size:2247; Vector Backbone:pICH41308; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30819783
Comments: Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_119612 Copy
Species: Synthetic
Genetic Insert: UP Flank Linker
Vector Backbone Description: Backbone Size:2247; Vector Backbone:pICH41331; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30819783
Comments: Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_119613 Copy
Species: Other
Genetic Insert: TrrnB
Vector Backbone Description: Backbone Size:2247; Vector Backbone:PICH41276; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30819783
Comments: E. coli double terminator rrnB T1 and T7Te. Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_119611 Copy
Species: Synthetic
Genetic Insert: AbR Down Linker
Vector Backbone Description: Backbone Size:2247; Vector Backbone:pICH1276; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30819783
Comments: Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_119616 Copy
Species: Synthetic
Genetic Insert: AbR Up Linker
Vector Backbone Description: Backbone Size:2247; Vector Backbone:pICH41331 ; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30819783
Comments: Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_119617 Copy
Species: Synthetic
Genetic Insert: Pcat
Vector Backbone Description: Backbone Size:2247; Vector Backbone:PICH41295; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30819783
Comments: Promoter for the chloramphenicol acetyl transferase (CAT) gene in Salmonella enterica. Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_119600 Copy
Species: Other
Genetic Insert: Tcpc_operon
Vector Backbone Description: Backbone Size:2247; Vector Backbone:PICH41276; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30819783
Comments: Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_119609 Copy
Species: Synthetic
Genetic Insert: J23108_TSS
Vector Backbone Description: Backbone Size:2247; Vector Backbone:PICH41295; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30819783
Comments: Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_119605 Copy
Species: Synthetic
Genetic Insert: J23103_TSS
Vector Backbone Description: Backbone Size:2247; Vector Backbone:PICH41295; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30819783
Comments: Addgene QC NGS identified sequence discrepancies in the plasmid insert. The plasmid is expected to function as described in the associated publication.
Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_119604 Copy
Genetic Insert: PgpdA::YFP::TtrpC(←)-PtrpC:nptII::Ttub (→)
Vector Backbone Description: Backbone Marker:Diego Orzaez; Backbone Size:7295; Vector Backbone:pDGB3omega1; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29680684
Proper citation: RRID:Addgene_119715 Copy
Species: Bacteriophage lambda
Genetic Insert: Red-alpha, Red-beta, Gam
Vector Backbone Description: Backbone Size:0; Vector Backbone:F'; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:16141338
Comments: pML300 carries the genes encoding the exo, beta, and gam recombination functions of bacteriophage lambda, as well as RecA, to render an E. coli strain recombination proficient. The lambda Red-alpha, Red-beta, and Gam proteins are under the rhaB promoter, induced by incubation with the sugar rhamnose. The bacterial strain is BW28705I (BUN21)/pML300 (recipient strain): lacIq rrnB3 delta-lacZ4787 hsdR514 delta-(araBAD)567 delta-(rhaBAD)568 galU95 delta-endA9::FRT delta-recA635::FRT umuC::ParaBAD-I-SceI-FRT + pML300(PrhaB-gam-beta-exo Ts(ori). Grow at 30'C in LB/Sp 50 ug/ml (spectinomycin resistant).
Proper citation: RRID:Addgene_11675 Copy
Species: Homo sapiens
Genetic Insert: Human MCM6
Vector Backbone Description: Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:25648893
Comments: Please note that mutation L657M in human MCM4 was found during Addgene's quality control. The depositor noted that this mutation does NOT affect plasmid function.
Proper citation: RRID:Addgene_116951 Copy
Species: Other
Genetic Insert: ncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30217854
Proper citation: RRID:Addgene_117236 Copy
Species: Streptococcus pyogenes
Genetic Insert: SpCas9-p (with stop codon)
Vector Backbone Description: Vector Backbone:pICH41308; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30811422
Comments: Compatible with MoClo, GB, Loop Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint
Proper citation: RRID:Addgene_117521 Copy
Species: Streptococcus pyogenes
Genetic Insert: SpCas9-h (no stop codon)
Vector Backbone Description: Vector Backbone:pAGM1287 ; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30811422
Comments: Compatible with Moclo, Loop Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint
Proper citation: RRID:Addgene_117520 Copy
Species: Streptococcus pyogenes
Genetic Insert: SpCas9-p (no stop codon)
Vector Backbone Description: Vector Backbone:pAGM1287; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30811422
Comments: Compatible with MoClo, GB, Loop Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint
Proper citation: RRID:Addgene_117522 Copy
Species: Streptococcus pyogenes
Genetic Insert: Sp-eCas9 1.1 (no stop codon)
Vector Backbone Description: Vector Backbone:pAGM1287; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30811422
Comments: Compatible with MoClo, Loop Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint
Proper citation: RRID:Addgene_117529 Copy
Species: Streptococcus pyogenes
Genetic Insert: Sp-eCas9 1.1 (with stop codon)
Vector Backbone Description: Vector Backbone:pICH41308; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30811422
Comments: Compatible with MoClo, Loop Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint
Proper citation: RRID:Addgene_117528 Copy
Species: Streptococcus pyogenes
Genetic Insert: SpCas9-KA (no stop codon)
Vector Backbone Description: Vector Backbone:pAGM1287; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30811422
Comments: Compatible with MoClo, Loop Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint
Proper citation: RRID:Addgene_117525 Copy
Species: Streptococcus pyogenes
Genetic Insert: Sp-eCas9 1.0 (no stop codon)
Vector Backbone Description: Vector Backbone:pAGM1287; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30811422
Comments: Compatible with MoClo, Loop Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint
Proper citation: RRID:Addgene_117527 Copy
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