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pC0.116
 
Resource Report
Resource Website 		RRID:Addgene_119612 Unmarked Linker Synthetic Spectinomycin PMID:30819783 Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint. Backbone Size:2247; Vector Backbone:pICH41308; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin 2026-08-15 01:03:13 0
pC0.117
 
Resource Report
Resource Website 		RRID:Addgene_119613 UP Flank Linker Synthetic Spectinomycin PMID:30819783 Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint. Backbone Size:2247; Vector Backbone:pICH41331; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin 2026-08-15 01:03:15 0
pC0.082
 
Resource Report
Resource Website 		RRID:Addgene_119611 TrrnB Other Spectinomycin PMID:30819783 E. coli double terminator rrnB T1 and T7Te. Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint. Backbone Size:2247; Vector Backbone:PICH41276; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin 2026-08-15 01:03:13 0
pC0.120
 
Resource Report
Resource Website 		RRID:Addgene_119616 AbR Down Linker Synthetic Spectinomycin PMID:30819783 Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint. Backbone Size:2247; Vector Backbone:pICH1276; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin 2026-08-15 01:03:15 0
pC0.121
 
Resource Report
Resource Website 		RRID:Addgene_119617 AbR Up Linker Synthetic Spectinomycin PMID:30819783 Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint. Backbone Size:2247; Vector Backbone:pICH41331 ; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin 2026-08-15 01:03:13 0
pC0.285
 
Resource Report
Resource Website 		RRID:Addgene_119600 Pcat Synthetic Spectinomycin PMID:30819783 Promoter for the chloramphenicol acetyl transferase (CAT) gene in Salmonella enterica. Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint. Backbone Size:2247; Vector Backbone:PICH41295; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin 2026-08-15 01:03:13 0
pC0.078
 
Resource Report
Resource Website 		RRID:Addgene_119609 Tcpc_operon Other Spectinomycin PMID:30819783 Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint. Backbone Size:2247; Vector Backbone:PICH41276; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin 2026-08-15 01:03:13 0
pC0.208
 
Resource Report
Resource Website 		RRID:Addgene_119605 J23108_TSS Synthetic Spectinomycin PMID:30819783 Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint. Backbone Size:2247; Vector Backbone:PICH41295; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin 2026-08-15 01:03:14 0
pC0.203
 
Resource Report
Resource Website 		RRID:Addgene_119604 J23103_TSS Synthetic Spectinomycin PMID:30819783 Addgene QC NGS identified sequence discrepancies in the plasmid insert. The plasmid is expected to function as described in the associated publication. Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint. Backbone Size:2247; Vector Backbone:PICH41295; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin 2026-08-15 01:03:13 0
FB039
 
Resource Report
Resource Website 		RRID:Addgene_119715 PgpdA::YFP::TtrpC(←)-PtrpC:nptII::Ttub (→) Spectinomycin PMID:29680684 Backbone Marker:Diego Orzaez; Backbone Size:7295; Vector Backbone:pDGB3omega1; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin 2026-08-15 01:03:15 0
BUN21/pML300 (recipient strain)
 
Resource Report
Resource Website 		RRID:Addgene_11675 Red-alpha, Red-beta, Gam Bacteriophage lambda Spectinomycin PMID:16141338 pML300 carries the genes encoding the exo, beta, and gam recombination functions of bacteriophage lambda, as well as RecA, to render an E. coli strain recombination proficient. The lambda Red-alpha, Red-beta, and Gam proteins are under the rhaB promoter, induced by incubation with the sugar rhamnose. The bacterial strain is BW28705I (BUN21)/pML300 (recipient strain): lacIq rrnB3 delta-lacZ4787 hsdR514 delta-(araBAD)567 delta-(rhaBAD)568 galU95 delta-endA9::FRT delta-recA635::FRT umuC::ParaBAD-I-SceI-FRT + pML300(PrhaB-gam-beta-exo Ts(ori). Grow at 30'C in LB/Sp 50 ug/ml (spectinomycin resistant). Backbone Size:0; Vector Backbone:F'; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:02:47 0
pCDF-MCM4/6
 
Resource Report
Resource Website 		RRID:Addgene_116951 Human MCM6 Homo sapiens Spectinomycin PMID:25648893 Please note that mutation L657M in human MCM4 was found during Addgene's quality control. The depositor noted that this mutation does NOT affect plasmid function. Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:02:49 0
pBECKP-spe
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_117236 ncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase Other Spectinomycin PMID:30217854 Vector Backbone:Unknown; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Spectinomycin 2026-08-15 01:02:52 1
pEPOR0SP0013
 
Resource Report
Resource Website 		RRID:Addgene_117521 SpCas9-p (with stop codon) Streptococcus pyogenes Spectinomycin PMID:30811422 Compatible with MoClo, GB, Loop Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint Vector Backbone:pICH41308; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin 2026-08-15 01:02:54 0
pICSL90005
 
Resource Report
Resource Website 		RRID:Addgene_117520 SpCas9-h (no stop codon) Streptococcus pyogenes Spectinomycin PMID:30811422 Compatible with Moclo, Loop Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint Vector Backbone:pAGM1287 ; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin 2026-08-15 01:02:54 0
pEPOR0SP0009
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_117522 SpCas9-p (no stop codon) Streptococcus pyogenes Spectinomycin PMID:30811422 Compatible with MoClo, GB, Loop Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint Vector Backbone:pAGM1287; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin 2026-08-15 01:02:54 1
pEPOR0SP0019
 
Resource Report
Resource Website 		RRID:Addgene_117529 Sp-eCas9 1.1 (no stop codon) Streptococcus pyogenes Spectinomycin PMID:30811422 Compatible with MoClo, Loop Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint Vector Backbone:pAGM1287; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin K848A, K1003A, R1060A 2026-08-15 01:02:54 0
pEPOR0SP0018
 
Resource Report
Resource Website 		RRID:Addgene_117528 Sp-eCas9 1.1 (with stop codon) Streptococcus pyogenes Spectinomycin PMID:30811422 Compatible with MoClo, Loop Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint Vector Backbone:pICH41308; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin K848A, K1003A, R1060A 2026-08-15 01:02:54 0
pEPOR0SP0015
 
Resource Report
Resource Website 		RRID:Addgene_117525 SpCas9-KA (no stop codon) Streptococcus pyogenes Spectinomycin PMID:30811422 Compatible with MoClo, Loop Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint Vector Backbone:pAGM1287; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin K855A 2026-08-15 01:02:54 0
pEPOR0SP0017
 
Resource Report
Resource Website 		RRID:Addgene_117527 Sp-eCas9 1.0 (no stop codon) Streptococcus pyogenes Spectinomycin PMID:30811422 Compatible with MoClo, Loop Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint Vector Backbone:pAGM1287; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin K810A, K1003A, R1060A 2026-08-15 01:02:54 0

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