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Species: Drosophila melanogaster
Genetic Insert: let-7–seed1-8
Vector Backbone Description: Backbone Marker:Zamore lab; Vector Backbone:pENTR/D-EGFP; Vector Types:Insect Expression, miRNA reporter; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20558712
Comments: The let-7 seed1-8 complimentary sequence was inserted in the XbaI site in the 3'UTR of EGFP using the following oligos:
s: CTAGATAACACGTTGGCTCTACCTCAT
as: CTAGATGAGGTAGAGCCAACGTGTTAT
Proper citation: RRID:Addgene_40843 Copy
Species: Drosophila melanogaster
Genetic Insert: let-7–mm16-21
Vector Backbone Description: Backbone Marker:Zamore lab; Vector Backbone:pENTR/D-EGFP; Vector Types:Insect Expression, miRNA reporter; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20558712
Comments: The let-7 mm16-21 complimentary sequence was inserted in the XbaI site in the 3'UTR of EGFP using the following oligos:
s: CTAGATGACACCAACCTACTACCTCAT
as: CTAGATGAGGTAGTAGGTTGGTGTCAT
Proper citation: RRID:Addgene_40849 Copy
Species: Drosophila melanogaster
Genetic Insert: let-7–Bartel
Vector Backbone Description: Backbone Marker:Zamore lab; Vector Backbone:pENTR/D-EGFP; Vector Types:Insect Expression, miRNA reporter; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20558712
Comments: The let-7 Bartel complimentary sequence was inserted in the XbaI site in the 3'UTR of EGFP using the following oligos:
s: CTAGATGATAACAACGATCTACCTCAT
as: CTAGATGAGGTAGATCGTTGTTATCAT
Proper citation: RRID:Addgene_40848 Copy
Species: Drosophila melanogaster
Genetic Insert: Four target sites perfectly complementary to let-7
Vector Backbone Description: Backbone Marker:Murhpy Lab, Carnegie Institution for Science; Backbone Size:7233; Vector Backbone:pAW; Vector Types:Insect Expression, miRNA reporter; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20558712
Comments: The let-7 complimentary sequence was inserted in the XbaI site in the 3'UTR of EGFP using the following oligos:
s: CTA GAA CTA TAC AAC CTA CTA CCT CAT CTA GAA CTA TAC AAC CTA CTA CCT CAT
as: CTA GAT GAG GTA GTA GGT TGT ATA GTT CTA GAT GAG GTA GTA GGT TGT ATA GTT
This expression cassette was then sub-cloned into the Gateway vector pAW using GATEWAY cloning.
Proper citation: RRID:Addgene_40834 Copy
Species: Drosophila melanogaster
Genetic Insert: Two target sites perfectly complementary to miR-1
Vector Backbone Description: Backbone Marker:Murhpy Lab, Carnegie Institution for Science; Backbone Size:7233; Vector Backbone:pAW; Vector Types:Insect Expression, miRNA reporter; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20558712
Comments: The miR-1 complimentary sequence was inserted in the XbaI site in the 3'UTR of EGFP using the following oligos:
s: CTA GAC TCC ATA CTT CTT TAC ATT CCA TCT AGA CTC CAT ACT TCT TTA CAT TCC AT
as: CTA GAT GGA ATG TAA AGA AGT ATG GAG TCT AGA TGG AAT GTA AAG AAG TAT GGA GT
This expression cassette was then sub-cloned into the Gateway vector pAW using GATEWAY cloning.
Proper citation: RRID:Addgene_40832 Copy
Species: Drosophila melanogaster
Genetic Insert: three sites partially complementary to miR-34
Vector Backbone Description: Backbone Marker:promega; Backbone Size:6273; Vector Backbone:psiCheck-2; Vector Types:Insect Expression, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22055293
Comments: The psiCheck-3xmiR-34 sequences were cloned by synthesized dsDNA
S: 5'-TCGAGGTGTTGATGCTAAGGTCACTGCCAGTGTTGATGCTAAGGTCACTGCCAGTGTTGATGCTAAGGTCACTGCCAGC
AS: 5'-GGCCGCTGGCAGTGACCTTAGCATCAACACTGGCAGTGACCTTAGCATCAACACTGGCAGTGACCTTAGCATCAACACC
the triple(3x)-repeated sequence:
5'TGGCAGTGACCTTAGCATCAACAC-3'
3'ACCGTCACTGGAATCGTAGTTGTG-5'
pairs with dme-miR-34 (uggcagugugguuagcugguugug) with "seed(red) plus 3' complementary region (blue)" fashion.
Proper citation: RRID:Addgene_40831 Copy
Species: Drosophila melanogaster
Genetic Insert: let-7–mm19-21
Vector Backbone Description: Backbone Marker:Zamore lab; Vector Backbone:pENTR/D-EGFP; Vector Types:Insect Expression, miRNA reporter; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20558712
Comments: The let-7–mm19-21 complimentary sequence was inserted in the XbaI site in the 3'UTR of EGFP using the following oligos:
s: CTAGATGAATACAACCTACTACCTCAT
as: CTAGATGAGGTAGTAGGTTGTATTCAT
Proper citation: RRID:Addgene_40839 Copy
Species: Drosophila melanogaster
Genetic Insert: let-7–mm21
Vector Backbone Description: Backbone Marker:Zamore lab; Vector Backbone:pENTR/D-EGFP; Vector Types:Insect Expression, miRNA reporter; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20558712
Comments: The let-7 mm21 complimentary sequence was inserted in the XbaI site in the 3'UTR of EGFP using the following oligos:
s: CTAGATCTATACAACCTACTACCTCAT
as: CTAGATGAGGTAGTAGGTTGTATAGAT
Proper citation: RRID:Addgene_40837 Copy
Species: Drosophila melanogaster
Genetic Insert: Loquacious-PA
Vector Backbone Description: Backbone Marker:Takara; Backbone Size:6000; Vector Backbone:pcold1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23063653
Proper citation: RRID:Addgene_41093 Copy
Species: Drosophila melanogaster
Genetic Insert: Loquacious-PB
Vector Backbone Description: Backbone Size:8000; Vector Backbone:pattB; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23063653
Proper citation: RRID:Addgene_41109 Copy
Species: Drosophila melanogaster
Genetic Insert: Loquacious-PA
Vector Backbone Description: Backbone Size:8000; Vector Backbone:pattB; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23063653
Proper citation: RRID:Addgene_41108 Copy
Species: Drosophila melanogaster
Genetic Insert: NSlmb-vhhGFP4
Vector Backbone Description: Backbone Size:8500; Vector Backbone:pUASattB; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22157958
Proper citation: RRID:Addgene_35577 Copy
Species: Drosophila melanogaster
Genetic Insert: NSlmb-vhhGFP4
Vector Backbone Description: Backbone Size:8800; Vector Backbone:pUAS; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22157958
Proper citation: RRID:Addgene_35575 Copy
Species: Drosophila melanogaster
Genetic Insert: NSnoFbox-vhhGFP4
Vector Backbone Description: Backbone Size:8500; Vector Backbone:pUASattB; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22157958
Proper citation: RRID:Addgene_35578 Copy
Species: Drosophila melanogaster
Genetic Insert: U6 and DR
Vector Backbone Description: Backbone Size:7350; Vector Backbone:white+attB; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33616113
Proper citation: RRID:Addgene_164586 Copy
Species: Drosophila melanogaster
Genetic Insert: R70B04
Vector Backbone Description: Backbone Size:2955; Vector Backbone:pUPD2; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34525356
Comments: Enhancer
Proper citation: RRID:Addgene_165792 Copy
Species: Drosophila melanogaster
Genetic Insert: Vasa promoter
Vector Backbone Description: Backbone Size:3057; Vector Backbone:pUPD; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34525356
Comments: Promoter
Proper citation: RRID:Addgene_165783 Copy
Species: Drosophila melanogaster
Genetic Insert: AlfaTub84b
Vector Backbone Description: Backbone Size:3057; Vector Backbone:pUPD; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34525356
Comments: Promoter
Proper citation: RRID:Addgene_165781 Copy
Species: Drosophila melanogaster
Genetic Insert: Hsp70b
Vector Backbone Description: Backbone Size:3057; Vector Backbone:pUPD; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34525356
Comments: Promoter
Proper citation: RRID:Addgene_165785 Copy
Species: Drosophila melanogaster
Genetic Insert: Hsp70b-TACT
Vector Backbone Description: Backbone Size:2955; Vector Backbone:pUPD2; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34525356
Comments: Promoter
Proper citation: RRID:Addgene_165780 Copy
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