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Species: Mus musculus
Genetic Insert: Rock2
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26368311
Proper citation: RRID:Addgene_101296 Copy
Genetic Insert: NL4-3 vpu
Vector Backbone Description: Vector Backbone:pALPS; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30546110
Proper citation: RRID:Addgene_101330 Copy
Species: Mus musculus
Genetic Insert: alpha-Catenin
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28329679
Comments: The TS module with mTFP1-F40-venusA206K was adapted from Vinculin TS (a kind gift from Martin Schwartz [Grashoff et al., 2010]). The TSmod was cloned in to pEGFP-N1 vector at Xho1 and Not1 restriction sites. For aE-Cat-TS, the mouse N-terminal (1_697 aa) of alpha-catenin was inserted before mTFP1 by in- fusion cloning (Takara Bioscience, Clontech) at the Xho1 restriction site
Proper citation: RRID:Addgene_101298 Copy
Species: Mus musculus
Genetic Insert: Rock1
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26368311
Proper citation: RRID:Addgene_101292 Copy
Species: Mus musculus
Genetic Insert: Rock1
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26368311
Proper citation: RRID:Addgene_101293 Copy
Species: Mus musculus
Genetic Insert: Rock1
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26368311
Proper citation: RRID:Addgene_101294 Copy
Genetic Insert: GFP, tat
Vector Backbone Description: Vector Backbone:pALPS; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30546110
Proper citation: RRID:Addgene_101338 Copy
Species: Mus musculus
Genetic Insert: alpha-Catenin
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28329679
Comments: The C-terminal fragment (698-906 aa) of alpha-catenin was cloned after VenusA206K at the Not1 restriction site
Proper citation: RRID:Addgene_101299 Copy
Genetic Insert: NL4-3 envFS
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30546110
Proper citation: RRID:Addgene_101333 Copy
Genetic Insert: NL4-3 envFS
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30546110
Proper citation: RRID:Addgene_101334 Copy
Genetic Insert: NL4-3
Vector Backbone Description: Vector Backbone:pNL4-3; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30546110
Proper citation: RRID:Addgene_101341 Copy
Genetic Insert: NL4-3
Vector Backbone Description: Vector Backbone:pNL4-4; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30546110
Comments: The last methionine in Gag (M423) was left as it was outside the ApaI site used to clone in the mutations. However, C-terminus of Gag is functionally inert, and Pol slips 27 nucleotides away and is unlikely to produce a stable protein product.
Proper citation: RRID:Addgene_101342 Copy
Genetic Insert: NL4-3
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30546110
Proper citation: RRID:Addgene_101349 Copy
Genetic Insert: NL4-3
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30546110
Proper citation: RRID:Addgene_101343 Copy
Genetic Insert: NL4-3
Vector Backbone Description: Vector Backbone:pNL4-4; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30546110
Proper citation: RRID:Addgene_101344 Copy
Species: Synthetic
Genetic Insert: ASAP2s
Vector Backbone Description: Backbone Size:4562; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28749338
Comments: The next-generation JEDI-2P sensors are available at: https://www.addgene.org/browse/article/28223314/
Proper citation: RRID:Addgene_101276 Copy
Species: Homo sapiens
Genetic Insert: METTL13
Vector Backbone Description: Backbone Marker:SGC (Addgene Plasmid #62304); Vector Backbone:pFBOH-MHL; Vector Types:Baculovirus Expression; Bacterial Resistance:Ampicillin
Comments: N terminal tag: MHHHHHHSSGRENLYFQG. SGC Clone Sample ID: METTL13:PBC007-B09:C235684. SGC or PDB link: http://www.thesgc.org/structures/5WCJ/
Proper citation: RRID:Addgene_101270 Copy
Species: Homo sapiens
Genetic Insert: EPHA3
Vector Backbone Description: Backbone Marker:SGC (Addgene Plasmid #26094); Vector Backbone:pET28a-LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: N terminal tag: MGSSHHHHHHSSGLVPRGS. SGC Clone Sample ID: EPHA3:SDC033-F08:C12121. SGC or PDB link: http://www.rcsb.org/pdb/explore/explore.do?structureId=2QO2
Proper citation: RRID:Addgene_101271 Copy
Genetic Insert: 4PY6
Vector Backbone Description: Backbone Marker:SGC (Addgene Plasmid #26092); Vector Backbone:pET15-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: N terminal tag: MHHHHHHSSGRENLYFQG. SGC Clone Sample ID: PFA0510w:MAC02W-B09:C201930. SGC or PDB link: http://www.rcsb.org/pdb/explore/explore.do?structureId=4PY6
Proper citation: RRID:Addgene_101272 Copy
Species: Mesoplasma florum
Genetic Insert: oriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
Vector Backbone Description: Vector Backbone:colE1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:28115382
Proper citation: RRID:Addgene_101316 Copy
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