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Species: Homo sapiens
Genetic Insert: RPS2_Keima
Vector Backbone Description: Vector Backbone:pLenti_C_Myc_DDK_IRES_Puro; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31981475
Proper citation: RRID:Addgene_127141 Copy
Species: Homo sapiens
Genetic Insert: Cas9-2A-eGFP
Vector Backbone Description: Vector Backbone:lentiCRISPR v2; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_127098 Copy
Species: Homo sapiens
Genetic Insert: RPS2
Vector Backbone Description: Vector Backbone:pLenti_C_Myc_DDK_IRES_Puro; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31981475
Proper citation: RRID:Addgene_127131 Copy
Species: Homo sapiens
Genetic Insert: RPS10
Vector Backbone Description: Vector Backbone:pLenti_C_Myc_DDK_IRES_Puro; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31981475
Comments: There is one additional mutation in RPS10- K24R. This point mutation does not affect function.
Proper citation: RRID:Addgene_127137 Copy
Species: Homo sapiens
Genetic Insert: RPS2_Keima
Vector Backbone Description: Vector Backbone:pLenti_C_Myc_DDK_IRES_Puro; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31981475
Proper citation: RRID:Addgene_127139 Copy
Species: Homo sapiens
Genetic Insert: RPS10
Vector Backbone Description: Vector Backbone:pLenti_C_Myc_DDK_IRES_Puro; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31981475
Proper citation: RRID:Addgene_127138 Copy
Species: Homo sapiens
Genetic Insert: EGFP - 3 repeats of human SUMO3 each separated by (GGS)4
Vector Backbone Description: Backbone Marker:unknown; Backbone Size:5000; Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27374333
Proper citation: RRID:Addgene_127093 Copy
Species: Homo sapiens
Genetic Insert: gRNA RNF123
Vector Backbone Description: Vector Backbone:px458_2A_GFP_sgRNA; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31981475
Proper citation: RRID:Addgene_127122 Copy
Species: Homo sapiens
Genetic Insert: gRNA ZNF598
Vector Backbone Description: Vector Backbone:px458_2A_GFP_sgRNA; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31981475
Proper citation: RRID:Addgene_127121 Copy
Species: Homo sapiens
Genetic Insert: gRNA LTN1
Vector Backbone Description: Vector Backbone:px458_2A_GFP_sgRNA; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31981475
Proper citation: RRID:Addgene_127125 Copy
Species: Homo sapiens
Genetic Insert: gRNA PELO
Vector Backbone Description: Vector Backbone:px458_2A_GFP_sgRNA; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31981475
Proper citation: RRID:Addgene_127124 Copy
Species: Homo sapiens
Genetic Insert: RPS10
Vector Backbone Description: Vector Backbone:pLenti_C_Myc_DDK_IRES_Puro; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31981475
Comments: Depositor confirms mutation does not affect function.
Proper citation: RRID:Addgene_127129 Copy
Species: Homo sapiens
Genetic Insert: gRNA G3BP1
Vector Backbone Description: Vector Backbone:px458_2A_GFP_sgRNA; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31981475
Proper citation: RRID:Addgene_127114 Copy
Species: Homo sapiens
Genetic Insert: gRNA G3BP2
Vector Backbone Description: Vector Backbone:px458_2A_GFP_sgRNA; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31981475
Proper citation: RRID:Addgene_127112 Copy
Species: Homo sapiens
Genetic Insert: gRNA UNK
Vector Backbone Description: Vector Backbone:px458_2A_GFP_sgRNA; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31981475
Proper citation: RRID:Addgene_127118 Copy
Species: Homo sapiens
Genetic Insert: FUS 1-163 S12xQ
Vector Backbone Description: Vector Backbone:RP1B; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31270472
Proper citation: RRID:Addgene_127193 Copy
Species: Homo sapiens
Genetic Insert: FUS 1-163
Vector Backbone Description: Vector Backbone:RP1B; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31270472
Proper citation: RRID:Addgene_127192 Copy
Species: Homo sapiens
Genetic Insert: G3BP2
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31981475
Proper citation: RRID:Addgene_127105 Copy
Species: Homo sapiens
Genetic Insert: WDR12
Vector Backbone Description: Backbone Marker:SGC (Addgene Plasmid #62304); Vector Backbone:pFBOH-MHL; Vector Types:Baculovirus Expression; Bacterial Resistance:Ampicillin
Comments: N terminal tag: MHHHHHHSSGRENLYFQG. SGC Clone Sample ID: WDR12:JMC061-H05:C224099. SGC or PDB link: http://www.thesgc.org/structures/6N31/
Proper citation: RRID:Addgene_127293 Copy
Species: Homo sapiens
Genetic Insert: HUWE1
Vector Backbone Description: Backbone Marker:SGC (Addgene Plasmid # 26096); Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:39048679
Comments: SGC or PDB links:
https://www.thesgc.org/structures/6MIW/
https://www.thesgc.org/structures/6PFL/
https://www.rcsb.org/structure/8RD7/
https://www.rcsb.org/structure/8R7O/
https://www.rcsb.org/structure/8RD0/
https://www.rcsb.org/structure/8RD1/
Proper citation: RRID:Addgene_127291 Copy
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