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Species: Synthetic
Genetic Insert: Insulated pBAD Promoter
Vector Backbone Description: Backbone Size:2023; Vector Backbone:DVA; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28422998
Proper citation: RRID:Addgene_78672 Copy
Species: Synthetic
Genetic Insert: Insulated pBAD Promoter
Vector Backbone Description: Backbone Size:2023; Vector Backbone:DVA; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28422998
Proper citation: RRID:Addgene_78675 Copy
Species: Synthetic
Genetic Insert: Insulated pBAD Promoter
Vector Backbone Description: Backbone Size:2023; Vector Backbone:DVA; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28422998
Proper citation: RRID:Addgene_78674 Copy
Species: Synthetic
Genetic Insert: amyEopt
Vector Backbone Description: Backbone Marker:Fagan and Fairweather; Backbone Size:6378; Vector Backbone:pRPF185; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27333161
Proper citation: RRID:Addgene_78707 Copy
Species: Synthetic
Genetic Insert: BBa_J23109-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0033-GFP(LVA)-Terminator
Vector Backbone Description: Backbone Marker:Registry of Standardized Biological Parts; Backbone Size:2047; Vector Backbone:pSB1C3; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27111289
Proper citation: RRID:Addgene_78703 Copy
Species: Synthetic
Genetic Insert: Insulated J23100 Promoter
Vector Backbone Description: Backbone Size:2023; Vector Backbone:DVA; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28422998
Proper citation: RRID:Addgene_78669 Copy
Species: Synthetic
Genetic Insert: BBa_J23109-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0033-GFP-Terminator
Vector Backbone Description: Backbone Marker:Registry of Standardized Biological Parts; Backbone Size:2047; Vector Backbone:pSB1C3; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27111289
Proper citation: RRID:Addgene_78704 Copy
Species: Synthetic
Genetic Insert: VC3AI
Vector Backbone Description: Backbone Size:7378; Vector Backbone:pCDH; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23857461
Comments: Usage information based on sensor VC3AI: VC3AI has no or little background fluorescence, and its activation depends on the cleavage of sensor sequence of DEVDG. In fact, the sensors containing DXXDG other than DEVDG do not work. However, cells expressing VC3AI may have “background” fluorescence in some conditions, and we can conform the so-called basal fluorescence still results from the cleaved VC3AI, because cells could have a low level of activated caspase3/7 in some conditions depending on cell line and culture condition.
To avoid the possible detectable basal fluorescence, we infect cells with different dilutions of lentivirus and use the stable cell line expressing sensor protein as high as possible but without basal fluorescence for experiments. Notably, the low level of sensor protein expression work poorly. During our investigation, the basal fluorescence often happens on GC3AI, if the sensor protein expression level is high. This is because GC3AI protein is more stable than other sensors.
Proper citation: RRID:Addgene_78907 Copy
Species: Synthetic
Genetic Insert: RC3AI
Vector Backbone Description: Backbone Size:7378; Vector Backbone:pCDH; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23857461
Comments: Usage information based on sensor VC3AI: VC3AI has no or little background fluorescence, and its activation depends on the cleavage of sensor sequence of DEVDG. In fact, the sensors containing DXXDG other than DEVDG do not work. However, cells expressing VC3AI may have “background” fluorescence in some conditions, and we can conform the so-called basal fluorescence still results from the cleaved VC3AI, because cells could have a low level of activated caspase3/7 in some conditions depending on cell line and culture condition.
To avoid the possible detectable basal fluorescence, we infect cells with different dilutions of lentivirus and use the stable cell line expressing sensor protein as high as possible but without basal fluorescence for experiments. Notably, the low level of sensor protein expression work poorly. During our investigation, the basal fluorescence often happens on GC3AI, if the sensor protein expression level is high. This is because GC3AI protein is more stable than other sensors.
Proper citation: RRID:Addgene_78908 Copy
Species: Synthetic
Genetic Insert: LbCpf1
Vector Backbone Description: Backbone Marker:NEB; Backbone Size:6000; Vector Backbone:pMAL-c5X; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27272384
Proper citation: RRID:Addgene_79008 Copy
Species: Synthetic
Genetic Insert: tBFP
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26830878
Comments: Precision Tumor Recognition by T Cells With Combinatorial Antigen-Sensing Circuits. Roybal KT, Rupp LJ, Morsut L, Walker WJ, McNally KA, Park JS, Lim WA. Cell. 2016 Feb 11;164(4):770-9. Epub 2016 Jan 28.
Proper citation: RRID:Addgene_79130 Copy
Species: Synthetic
Genetic Insert: PlacZ-opt (off-target) luxAB; Ppro1 434cI-SH2ABL1
Vector Backbone Description: Backbone Marker:David Liu Lab; Backbone Size:2992; Vector Backbone:SC101; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27120167
Proper citation: RRID:Addgene_79205 Copy
Species: Synthetic
Genetic Insert: PlacZ-opt (OR1) gIII, luxAB; Ppro1 434cI(RR69)-SH2ABL1
Vector Backbone Description: Backbone Marker:David Liu Lab; Backbone Size:2992; Vector Backbone:SC101; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27120167
Proper citation: RRID:Addgene_79201 Copy
Species: Synthetic
Genetic Insert: PlacZ-opt (no operator) gIII, luxAB; Ppro1 434cI-SH2ABL1
Vector Backbone Description: Backbone Marker:David Liu Lab; Backbone Size:2992; Vector Backbone:SC101; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27120167
Proper citation: RRID:Addgene_79200 Copy
Species: Synthetic
Genetic Insert: PlacZ-opt (OR1) luxAB; Ppro1 434cI-SH2ABL1
Vector Backbone Description: Backbone Marker:David Liu Lab; Backbone Size:2992; Vector Backbone:SC101; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27120167
Proper citation: RRID:Addgene_79203 Copy
Species: Synthetic
Genetic Insert: PlacZ-opt (OR1) luxAB; Ptet 434cI-TnTBR3-F3
Vector Backbone Description: Backbone Marker:David Liu Lab; Backbone Size:2992; Vector Backbone:SC101; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27120167
Proper citation: RRID:Addgene_79209 Copy
Species: Synthetic
Genetic Insert: PlacZ HA4-Zif268; PlacIq lacI
Vector Backbone Description: Backbone Marker:David Liu Lab; Backbone Size:2992; Vector Backbone:ColEI; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:27120167
Proper citation: RRID:Addgene_79221 Copy
Species: Synthetic
Genetic Insert: PlacZ lambda cI-SH2ABL1; PlacIq lacI
Vector Backbone Description: Backbone Marker:David Liu Lab; Backbone Size:2992; Vector Backbone:ColEI; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:27120167
Proper citation: RRID:Addgene_79220 Copy
Species: Synthetic
Genetic Insert: Ppro1 rpoZ-HA4; PlacIq lacI
Vector Backbone Description: Backbone Marker:David Liu Lab; Backbone Size:2992; Vector Backbone:ColEI; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:27120167
Proper citation: RRID:Addgene_79226 Copy
Species: Synthetic
Genetic Insert: PlacZ lambda cI-MBP; PlacIq lacI
Vector Backbone Description: Backbone Marker:David Liu Lab; Backbone Size:2992; Vector Backbone:ColEI; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:27120167
Proper citation: RRID:Addgene_79222 Copy
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