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Species: Synthetic
Genetic Insert: anti-vimentin-nanobody VB6 (NbVim) tagged with circularly permutated superfolderGFP(cp10GFP)
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:3300; Vector Backbone:modified pcDNA3.1; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34090210
Proper citation: RRID:Addgene_171020 Copy
Species: Homo sapiens
Genetic Insert: LargeBit-SNAPtag- Beta-actin
Vector Backbone Description: Backbone Marker:System Biosciences; Backbone Size:7657; Vector Backbone:pCDH; Vector Types:Mammalian Expression, Lentiviral, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34140497
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.08.20.258350v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_171019 Copy
Species: Synthetic
Genetic Insert: 14x-His-bdSUMO-HA2-muGFP-HiBiT
Vector Backbone Description: Backbone Size:4694; Vector Backbone:pET His6 MBP TEV LIC cloning vector (2M-T); Vector Types:Bacterial Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34140497
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.08.20.258350v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_171017 Copy
Species: Synthetic
Genetic Insert: 14x-His-bdSUMO-pHD118-muGFP-HiBiT
Vector Backbone Description: Backbone Size:4694; Vector Backbone:pET His6 MBP TEV LIC cloning vector (2M-T); Vector Types:Bacterial Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34140497
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.08.20.258350v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_171015 Copy
Species: Synthetic
Genetic Insert: 14x-His-bdSUMO-5.3-muGFP-HiBiT
Vector Backbone Description: Backbone Size:4694; Vector Backbone:pET His6 MBP TEV LIC cloning vector (2M-T); Vector Types:Bacterial Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34140497
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.08.20.258350v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_171014 Copy
Species: Homo sapiens
Genetic Insert: U6:sgRNA_Puro-T2A-BFP
Vector Backbone Description: Backbone Size:9000; Vector Backbone:pCRISPRia-v2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34414886
Proper citation: RRID:Addgene_171011 Copy
Species: Mus musculus
Genetic Insert: hSpCas9
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid # 48138); Vector Backbone:PX458; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34143204
Proper citation: RRID:Addgene_171099 Copy
Species: Synthetic
Genetic Insert: EGFP-uTEV1-SQS(FL)
Vector Backbone Description: Backbone Size:8000; Vector Backbone:pCW-Cas9; Vector Types:Mammalian Expression, Lentiviral, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34414886
Proper citation: RRID:Addgene_171010 Copy
Species: Homo sapiens
Genetic Insert: OsTIR1-mEmerald-Cdt1-P2A-miniAID-mCherry2
Vector Backbone Description: Backbone Marker:Eric Campeau, Paul Kaufman; Vector Backbone:pLenti CMV Blast DEST (706-1); Vector Types:Mammalian Expression, Lentiviral, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37537305
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.04.23.441203v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_171096 Copy
Species: Homo sapiens
Genetic Insert: OsTIR1-mEmerald-P2A-miniAID-mCherry2
Vector Backbone Description: Backbone Marker:Eric Campeau, Paul Kaufman; Vector Backbone:pLenti CMV Blast DEST (706-1); Vector Types:Mammalian Expression, Lentiviral, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37537305
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.04.23.441203v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_171094 Copy
Species: Caenorhabditis elegans
Genetic Insert: pgl-3
Vector Backbone Description: Vector Backbone:pMAL-c2X; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.10.15.340570v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_171208 Copy
Species: Caenorhabditis elegans
Genetic Insert: meg-3
Vector Backbone Description: Vector Backbone:pGEX-6p-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.10.15.340570v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_171203 Copy
Genetic Insert: GCaMP6s
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35120626
Proper citation: RRID:Addgene_171156 Copy
Genetic Insert: ChroME2f-P2A-H2B-mRuby3
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35120626
Proper citation: RRID:Addgene_171150 Copy
Species: Homo sapiens
Genetic Insert: human androgen receptor Ser256Ala mutant
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5500; Vector Backbone:pCIneo; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17043241
Comments: Mutation in an AR Ser-Pro phosphorylation site, and the exact amino acid residue position and composition may slightly vary based on human AR versions.
Proper citation: RRID:Addgene_171227 Copy
Species: Homo sapiens
Genetic Insert: human androgen receptor Ser81Glu mutant
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5500; Vector Backbone:pCIneo; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17043241
Comments: Mutation in an AR Ser-Pro phosphorylation site, and the exact amino acid residue position and composition may slightly vary based on human AR versions.
Proper citation: RRID:Addgene_171224 Copy
Species: Homo sapiens
Genetic Insert: human androgen receptor Ser81Ala mutant
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5500; Vector Backbone:pCIneo; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Mutation in an AR Ser-Pro phosphorylation site, and the exact amino acid residue position and composition may slightly vary based on human AR versions.
Proper citation: RRID:Addgene_171223 Copy
Species: Homo sapiens
Genetic Insert: AR-C619Y
Vector Backbone Description: Backbone Size:10000; Vector Backbone:Lenti-viral-Hygro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34911936
Comments: The mutation occurs at the end of androgen receptor zinc finger 2 and this AR mutant is defective in transactivation but not nuclear import.
Human androgen receptor reference: https://pubmed.ncbi.nlm.nih.gov/18319300/
Please Note: This plasmid contains a 6x Gln deletion in the 23x Gln track and a 8x Gly deletion in the 23x Gly track in androgen receptor compared to the depositor's provided sequence. These differences are not known to affect the function of the protein.
Proper citation: RRID:Addgene_171222 Copy
Species: Homo sapiens
Genetic Insert: AR-R599A-N600A
Vector Backbone Description: Backbone Size:10000; Vector Backbone:Lenti-viral-Hygro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34911936
Comments: The mutations occur in the D-box of the androgen receptor Zinc finger 2 that mediates AR dimerization.
Please Note: This plasmid contains a 6x Gln deletion in the 23x Gln track and a 8x Gly deletion in the 23x Gly track in androgen receptor compared to the depositor's provided sequence. These differences are not known to affect the function of the protein.
Proper citation: RRID:Addgene_171221 Copy
Species: Mus musculus
Genetic Insert: hU6-sgLacZ1-hU6-sgLacZ2
Vector Backbone Description: Backbone Size:5294; Vector Backbone:pAAV-hU6-gRNA-hSyn-mCherry-KASH; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33510438
Comments: Please note Addgene NGS identified two mutations in mCherry (K14E, N210D) and one in KASH domain of Nesprin2 E>G. Depositor confirms plasmid is functional.
Proper citation: RRID:Addgene_171185 Copy
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