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Vector Backbone Description: Backbone Size:7027; Vector Backbone:pQCXIB; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19657394
Proper citation: RRID:Addgene_17487 Copy
Species: Synthetic
Genetic Insert: humanized RfxCas13d
Vector Backbone Description: Vector Backbone:pCX539; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33941935
Proper citation: RRID:Addgene_171380 Copy
Species: Homo sapiens
Genetic Insert: Tom20 (1-34 aa)-ECFP-FRB (2021-2113 aa)
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pECFP-C1 (modified); Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35475143
Proper citation: RRID:Addgene_171461 Copy
Species: Homo sapiens
Genetic Insert: NbVHH05-GFP
Vector Backbone Description: Vector Backbone:pAW; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35076390
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.04.16.440240v1 for BioRxiv preprint.
Proper citation: RRID:Addgene_171570 Copy
Species: Homo sapiens
Genetic Insert: H2B
Vector Backbone Description: Vector Backbone:pGEHME; Vector Types:pGEHME; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33964210
Proper citation: RRID:Addgene_171489 Copy
Vector Backbone Description: Backbone Marker:Moon Lab; Backbone Size:0; Vector Backbone:pCS2+mRFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Notes for Use:
For making amino-terminal mRFP fusion protein, reading frame 1, in vector CS2+.
Made by: Jeff Miller.
Proper citation: RRID:Addgene_17143 Copy
Species: Xenopus laevis
Genetic Insert: Beta-tubulin promoter
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:4300; Vector Backbone:pBLCAT3; Vector Types:; Bacterial Resistance:Ampicillin
Comments: Constructed Rebecca Beach and Paul A. Kreig.
3.8Kb fragment of the Xenopus neural Beta-tubulin promoter inserted (in several cloning steps) into the CAT expression vector pBLCAT3. The 5' end of the neural Beta-tubulin sequence is defined by the natural Xba1 site that occurs in the genomic DNA. The 3' end of the insert is produced by PCR primer located 8bp upstream of hte initiation ATP and 120bp downstream of the transcription start site. The neural Beta-tubulin sequences may be excised as a Hind3 fragment.
Proper citation: RRID:Addgene_17146 Copy
Species: Xenopus laevis
Genetic Insert: cardiac actin promoter
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCSKA; Vector Types:Xenopus expression; Bacterial Resistance:Ampicillin
Comments: Plasmid History:
Constructed by Enrique Amaya.
Note for Use:
Constructed by cloning the 3270bp KpnI-SalI fragment from DNA#254 (from Tim Mohun) into KpnI-SalI digested pCSKA.
Proper citation: RRID:Addgene_17148 Copy
Species: Synthetic
Genetic Insert: ULK1
Vector Backbone Description: Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33893090
Proper citation: RRID:Addgene_171416 Copy
Species: Homo sapiens
Genetic Insert: UBDUB gRNA
Vector Backbone Description: Backbone Marker:addgene: #52963; Vector Backbone:LentiGuide-Puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37036970
Proper citation: RRID:Addgene_171531 Copy
Species: Homo sapiens
Genetic Insert: WIPI2d
Vector Backbone Description: Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33893090
Proper citation: RRID:Addgene_171419 Copy
Species: Mus musculus
Genetic Insert: H3.3B
Vector Backbone Description: Vector Backbone:pGEHME; Vector Types:pGEHME; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33964210
Proper citation: RRID:Addgene_171496 Copy
Species: Synthetic
Genetic Insert: C- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
Vector Backbone Description: Vector Backbone:PINII; Vector Types:Multisite Gateway entry vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34211131
Comments: Multisite Gateway entry vector to express one hand of pTALECD-UGI. Use Platinum Gate TALEN Kit (Kit # 1000000043 ) for assembly of TALE repeats, and use this plasmid in last step.
Proper citation: RRID:Addgene_171724 Copy
Genetic Insert: PCnU6-sgRNA_scaffold
Vector Backbone Description: Backbone Size:4866; Vector Backbone:pRS316; Vector Types:Bacterial Expression, Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34791226
Proper citation: RRID:Addgene_171686 Copy
Species: Homo sapiens
Genetic Insert: Ratiometric pHLuorin2 fused to B4GALT1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4730; Vector Backbone:pRpHluorin-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35000377
Comments: Ratiometric pHLuorin2 sequence synthesized by Genscript, original sequence from https://www.scirp.org/journal/paperinformation.aspx?paperid=5043
Proper citation: RRID:Addgene_171719 Copy
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4730; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35000377
Comments: Ratiometric pHLuorin2 sequence synthesized by Genscript, original sequence from https://www.scirp.org/journal/paperinformation.aspx?paperid=5043
Proper citation: RRID:Addgene_171716 Copy
Species: Synthetic
Genetic Insert: TRE-NpHR-EYFP
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36509775
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.10.13.464095v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_171621 Copy
Species: Homo sapiens
Genetic Insert: 3x127D01
Vector Backbone Description: Vector Backbone:pScarlessHD; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35076390
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.04.16.440240v1 for BioRxiv preprint.
Proper citation: RRID:Addgene_171578 Copy
Species: Other
Genetic Insert: AsCas12f1
Vector Backbone Description: Vector Backbone:p15a; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
Defining Citation: PMID:34475565
Proper citation: RRID:Addgene_171610 Copy
Species: Homo sapiens
Genetic Insert: BEACON2
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32492431
Proper citation: RRID:Addgene_171698 Copy
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