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Species: Synthetic
Genetic Insert: EGFP
Vector Backbone Description: Backbone Marker:Zhang Lab (Addgene plasmid # 52963); Vector Backbone:lentiGuide-Puro; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26780180
Comments: Note that this plasmid does NOT contain Cas9. It should be used in conjunction with lentiCas9-Blast (Addgene #52962) or otherwise with cell lines already expressing Cas9. Browse the full kinome gRNA library at http://www.addgene.org/pooled-library/broadgpp-human-kinome/ or visit http://www.broadinstitute.org/rnai/public/ for detailed protocols and information.
Proper citation: RRID:Addgene_80034 Copy
Species: Synthetic
Genetic Insert: EPN-01(deltaYP)
Vector Backbone Description: Backbone Marker:...; Backbone Size:5478; Vector Backbone:pCMV(WT); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27919066
Proper citation: RRID:Addgene_80050 Copy
Species: Synthetic
Genetic Insert: pCMV-EPN-01*(ΔL2)
Vector Backbone Description: Backbone Marker:...; Backbone Size:5478; Vector Backbone:pCMV(WT); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27919066
Proper citation: RRID:Addgene_80042 Copy
Species: Synthetic
Genetic Insert: EPN-01(L33R)
Vector Backbone Description: Backbone Marker:...; Backbone Size:5478; Vector Backbone:pCMV(WT); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27919066
Comments: Please note that the L33R mutation is numbered according to the original I3-01 protein and is L50R when numbered according to EPN-01.
Proper citation: RRID:Addgene_80048 Copy
Species: Synthetic
Genetic Insert: EPN-01*(L33R)
Vector Backbone Description: Backbone Marker:...; Backbone Size:5478; Vector Backbone:pCMV(WT); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27919066
Proper citation: RRID:Addgene_80044 Copy
Species: Synthetic
Genetic Insert: mNeonGreen2(1-10)
Vector Backbone Description: Backbone Size:9000; Vector Backbone:pHR-SFFV; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28851864
Proper citation: RRID:Addgene_82610 Copy
Species: Synthetic
Genetic Insert: mNeonGreen2(1-10)_32aalinker_mNeonGreen2(11)
Vector Backbone Description: Backbone Size:5296; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28851864
Comments: In the mNG2(1-10) fragment, mutations are K128M, S142T, R150M, G172V and K213M; in the mNG2(11) fragment, mutation is V15M.
Proper citation: RRID:Addgene_82611 Copy
Species: Synthetic
Genetic Insert: 3xFLAG-dCas9
Vector Backbone Description: Backbone Size:6421; Vector Backbone:MSCV-EGFP; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29480524
Proper citation: RRID:Addgene_82613 Copy
Species: Synthetic
Genetic Insert: sfCherry2(1-10)
Vector Backbone Description: Backbone Size:9000; Vector Backbone:pHR-SFFV; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28851864
Proper citation: RRID:Addgene_82603 Copy
Species: Synthetic
Genetic Insert: sfCherry2(11)_TagBFP
Vector Backbone Description: Backbone Size:9000; Vector Backbone:pHR-SFFV; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28851864
Proper citation: RRID:Addgene_82606 Copy
Species: Synthetic
Genetic Insert: H2B-mCherry and H2B-Citrine. HygroR
Vector Backbone Description: Backbone Size:1950; Vector Backbone:SMX DEST vector Addgene 62399; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27152616
Proper citation: RRID:Addgene_82478 Copy
Species: Synthetic
Genetic Insert: eGFP-IRESMeo
Vector Backbone Description: Vector Backbone:pGAPTrap; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27594589
Proper citation: RRID:Addgene_82502 Copy
Species: Synthetic
Genetic Insert: eGFP-IRESMuro
Vector Backbone Description: Vector Backbone:pGAPTrap; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27594589
Proper citation: RRID:Addgene_82504 Copy
Species: Synthetic
Genetic Insert: GFP Nanobody
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5475; Vector Backbone:pBiex1; Vector Types:Bacterial Expression, Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27538185
Proper citation: RRID:Addgene_82711 Copy
Species: Synthetic
Genetic Insert: shRNA Neg Control
Vector Backbone Description: Backbone Marker:Addgene; Vector Backbone:pLVTHM; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28969075
Proper citation: RRID:Addgene_83046 Copy
Species: Synthetic
Genetic Insert: sfCherry+full length_TagBFP
Vector Backbone Description: Backbone Size:9000; Vector Backbone:pHR-SFFV; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28851864
Proper citation: RRID:Addgene_83031 Copy
Species: Synthetic
Genetic Insert: Su9-roGFP2-Tsa2dCr
Vector Backbone Description: Vector Backbone:p415-TEF; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27089028
Proper citation: RRID:Addgene_83240 Copy
Species: Synthetic
Genetic Insert: roGFP2-Tsa2dCpdCr
Vector Backbone Description: Vector Backbone:p415-TEF; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27089028
Proper citation: RRID:Addgene_83239 Copy
Species: Synthetic
Genetic Insert: roGFP2-Tsa2dCr
Vector Backbone Description: Vector Backbone:p415-TEF; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27089028
Proper citation: RRID:Addgene_83238 Copy
Species: Synthetic
Genetic Insert: eGFP(1-155)
Vector Backbone Description: Backbone Marker:Kodama and Wada 2009; Backbone Size:3459; Vector Backbone:pBRN169-MXMT; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_80130 Copy
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