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Species: Feline Immunodeficiency Virus
Genetic Insert: Gag-Pol and Rev
Vector Backbone Description: Backbone Size:12905; Vector Backbone:modified pUC119; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: This is the FIV PPR based second generation packaging vector, part of the FELIX vector system from Nolan lab. The FELIX vector system consists of three plasmids - structural, envelope, and transfer vector - which are cotransfected into 293T cells to produce virus. The structural construct makes Gag-Pol and Rev (some variants also make Vif and A). Any envelope construct can be used for pseudotyping - VSV-G tends to give the highest efficiency and broadest host range. The transfer vector contains the viral LTRs and packaging signal. Any expression cassette (promoter and gene of interest) can be cloned into the vector. Inserts can be up to 8.5kB. Please note that the sequence information has some inaccuracies, so restriction digests may give slightly different bands than predicted.
Proper citation: RRID:Addgene_1732 Copy
Species: Mus musculus
Genetic Insert: Delta-like 4
Vector Backbone Description: Backbone Marker:Conaway Lab; Backbone Size:5500; Vector Backbone:pBacPAK8; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18077452
Proper citation: RRID:Addgene_17335 Copy
Genetic Insert: LacZ
Vector Backbone Description: Backbone Marker:Nolan Lab; Backbone Size:8396; Vector Backbone:pBMN; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Comments: This is Nolan lab's standard retrovirus vector backbone that expresses lacZ. Most of the other library vectors and IRES constructs are based on this expression vector. A variant comes with GFP in place of the lacZ gene (see pBMN-I-GFP).
Please note that there are some minor differences between Addgene's quality control sequence (see sequence with the pLXSN-5 primer) and the assembled sequence from the Nolan lab. See the following reference for further information: digitalcommons.unl.edu/cgi/viewcontent.cgi?article=1008&context=chemistrycerny
Proper citation: RRID:Addgene_1734 Copy
Species: Rattus norvegicus
Genetic Insert: Jagged1
Vector Backbone Description: Backbone Marker:BD Biosciences; Backbone Size:6100; Vector Backbone:pIRES; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18077452
Comments: The backbone of this plasmid appears to be approximately 600bp smaller than is reported. The depositing scientist is aware of this difference and reports that the plasmid functions as it should.
Proper citation: RRID:Addgene_17336 Copy
Genetic Insert: mCherry-mNeonGreen
Vector Backbone Description: Backbone Size:6711; Vector Backbone:pRS41K-CEN ARS kanMX (type II donor template) (pMaM484); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34985695
Proper citation: RRID:Addgene_173460 Copy
Species: Synthetic
Genetic Insert: MCP-mCherry-GFP11-GB1
Vector Backbone Description: Backbone Size:6589; Vector Backbone:pHAGE-DEST; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32887690
Proper citation: RRID:Addgene_173216 Copy
Genetic Insert: mCherry-sfGFP
Vector Backbone Description: Backbone Size:5230; Vector Backbone:pRS41K-CEN ARS kanMX (type I donor template); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34985695
Proper citation: RRID:Addgene_173457 Copy
Species: Mus musculus
Genetic Insert: Autophagy Related 7
Vector Backbone Description: Backbone Marker:Cell Biolabs; Backbone Size:5600; Vector Backbone:pMXS-IRES-Blast; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33152324
Proper citation: RRID:Addgene_173170 Copy
Species: Homo sapiens
Genetic Insert: eIF4E
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17289916
Comments: RT-PCR product of mRNA from human A549 cell.
Proper citation: RRID:Addgene_17343 Copy
Species: Homo sapiens
Genetic Insert: 4EHP
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17289916
Proper citation: RRID:Addgene_17344 Copy
Species: Homo sapiens
Genetic Insert: 4EHP
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:4700; Vector Backbone:pFlag-CMV2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17289916
Proper citation: RRID:Addgene_17345 Copy
Species: Homo sapiens
Genetic Insert: ATP1A1 G4 Q118R pegRNA + RUNX1 +1 ATG insertion pegRNA
Vector Backbone Description: Vector Backbone:pU6-pegRNA-GG-acceptor; Vector Types:Mammalian Expression, CRISPR, Prime editing; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36207338
Comments: Control pegRNA vector derived from ATP1A1_G4_Q118R_Dual_pegRNA. This vector can be used as a positive control for prime editing (PE3 in combination with ATP1A1_G3_RUNX1_Nick-38_Dual_sgRNA) to co-target ATP1A1 and RUNX1 to perform coselection for RUNX1 +1 ATG insertion. Please visit https://doi.org/10.1101/2021.11.02.464583 for bioRxiv preprint.
Proper citation: RRID:Addgene_173213 Copy
Genetic Insert: mScarlet-mNeonGreen
Vector Backbone Description: Backbone Size:5614; Vector Backbone:pFA6a-mCherry-sfGFP-kanMX (pMaM17); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34985695
Proper citation: RRID:Addgene_173455 Copy
Species: Synthetic
Genetic Insert: gRNA-12xMBS
Vector Backbone Description: Backbone Size:7514; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32887690
Proper citation: RRID:Addgene_173178 Copy
Species: Homo sapiens
Genetic Insert: ATP1A1 G3 sgRNA + MTOR Nick exon-53 sgRNA
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Mammalian Expression, CRISPR, Prime editing (PE3); Bacterial Resistance:Ampicillin
Defining Citation: PMID:36207338
Comments: Control PE3 nick sgRNAs vector derived from ATP1A1_G3_Dual_sgRNA. This vector can be used as a positive control for prime editing (PE3) to co-target ATP1A1 and MTOR to perform coselection for MTOR hyperactivating mutation E2419K. Please visit https://doi.org/10.1101/2021.11.02.464583 for bioRxiv preprint.
Proper citation: RRID:Addgene_173211 Copy
Species: Escherichia coli
Genetic Insert: E. coli E69 (O9a:K30) Wzc_K540M with Y717E, Y718E
Vector Backbone Description: Backbone Size:4542; Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34272394
Proper citation: RRID:Addgene_173451 Copy
Species: Synthetic
Genetic Insert: gRNA-12xMBS backbone
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4837; Vector Backbone:pGL3-Basic; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32887690
Proper citation: RRID:Addgene_173175 Copy
Species: Escherichia coli
Genetic Insert: E. coli E69 (O9a:K30) Wzc_K540M with Y718E
Vector Backbone Description: Backbone Size:4542; Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34272394
Proper citation: RRID:Addgene_173450 Copy
Species: Synthetic
Genetic Insert: dCas9
Vector Backbone Description: Backbone Size:6479; Vector Backbone:pHAGE-DEST; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32887690
Proper citation: RRID:Addgene_173173 Copy
Species: Mus musculus
Genetic Insert: Neuralized-like
Vector Backbone Description: Backbone Marker:Conaway Lab; Backbone Size:5600; Vector Backbone:pcDNA3.1 C-FLAG; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18077452
Proper citation: RRID:Addgene_17316 Copy
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