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Species: Homo sapiens
Genetic Insert: mISG15-4EHP
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:4700; Vector Backbone:pFlag-CMV2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17289916
Comments: pFLAG-mISG15-4EHP expresses a FLAG tagged mISG15(LRLRG)-human 4EHP fusion protein. mISG15, which does not contain a STOP codon, was made by PCR and subcloned into the HindIII site of pFLAG-4EHP.
Proper citation: RRID:Addgene_17354 Copy
Species: Homo sapiens
Genetic Insert: 4EHP-mISG15
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:4700; Vector Backbone:pFlag-CMV2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17289916
Comments: pFLAG-4EHP-mISG15 expresses a FLAG tagged human 4EHP-mISG15(LRLRGG) fusion protein. STOP codon of 4EHP was removed by PCR. BamHI-mISG15-XbaI, which was made by PCR, was ligated to pFLAG-4EHP(stopless)/BamHI/XbaI sites.
Proper citation: RRID:Addgene_17355 Copy
Species: E. coli transposon Tn7
Genetic Insert: TnsA
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:6800; Vector Backbone:pCYB2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10911996
Proper citation: RRID:Addgene_17356 Copy
Genetic Insert: IRES-GFP
Vector Backbone Description: Backbone Marker:Nolan Lab; Backbone Size:6332; Vector Backbone:pBMN; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Comments: The pBMN backbone with IRES allowing for expression of GFP. Please note that the sequence information has some inaccuracies, so restriction digests may give slightly different bands than predicted.
This plasmid is extremely slow growing and may need 2 days to grow.
Proper citation: RRID:Addgene_1736 Copy
Species: Caenorhabditis elegans
Genetic Insert: Prps-0::CIBN-3xFLAG::MP::unc-54 3' UTR
Vector Backbone Description: Backbone Marker:Agilent Technologies; Backbone Size:2999; Vector Backbone:pMLS257; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34849800
Comments: Please visit https://doi.org/10.1101/2021.06.01.446599 for bioRxiv preprint.
Proper citation: RRID:Addgene_173744 Copy
Species: Caenorhabditis elegans
Genetic Insert: Prps-0::mKate2::CRY2(olig)::VHH(GFP)::unc-54 3' UTR
Vector Backbone Description: Backbone Marker:Agilent Technologies; Backbone Size:2999; Vector Backbone:pMLS257; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34849800
Comments: Please visit https://doi.org/10.1101/2021.06.01.446599 for bioRxiv preprint.
Proper citation: RRID:Addgene_173745 Copy
Species: Caenorhabditis elegans
Genetic Insert: Prps-0::CRY2(olig)::VHH(mCherry)::unc-54 3' UTR
Vector Backbone Description: Backbone Marker:Agilent Technologies; Backbone Size:2999; Vector Backbone:pMLS257; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34849800
Comments: Please visit https://doi.org/10.1101/2021.06.01.446599 for bioRxiv preprint.
Proper citation: RRID:Addgene_173747 Copy
Species: Caenorhabditis elegans
Genetic Insert: Pwrt-2::CRY2(olig)::VHH(GFP)::unc-54 3' UTR
Vector Backbone Description: Backbone Marker:Agilent Technologies; Backbone Size:2999; Vector Backbone:pMLS257; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34849800
Comments: Please visit https://doi.org/10.1101/2021.06.01.446599 for bioRxiv preprint.
Proper citation: RRID:Addgene_173748 Copy
Species: Other
Genetic Insert: A gRNA targeting the dog Hbegf gene and the cDNA of CRISPR-Cas9
Vector Backbone Description: Backbone Marker:https://www.addgene.org/48139/; Backbone Size:3200; Vector Backbone:pX459; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34667080
Proper citation: RRID:Addgene_173699 Copy
Species: Other
Genetic Insert: A gRNA targeting the dog Nrg1 gene and the cDNA of CRISPR-Cas9
Vector Backbone Description: Backbone Marker:https://www.addgene.org/48139/; Backbone Size:3200; Vector Backbone:pX459; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34667080
Proper citation: RRID:Addgene_173696 Copy
Species: Other
Genetic Insert: A gRNA targeting the dog Ereg gene and the cDNA of CRISPR-Cas9
Vector Backbone Description: Backbone Marker:https://www.addgene.org/48139/; Backbone Size:3200; Vector Backbone:pX459; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34667080
Proper citation: RRID:Addgene_173697 Copy
Species: Caenorhabditis elegans
Genetic Insert: Prps-0::CRY2(olig)::VHH(GFP)::T2A::CIBN-3xFLAG::MP::unc-54 3' UTR
Vector Backbone Description: Backbone Marker:Agilent Technologies; Backbone Size:2999; Vector Backbone:pMLS257; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34849800
Comments: Please visit https://doi.org/10.1101/2021.06.01.446599 for bioRxiv preprint.
Proper citation: RRID:Addgene_173753 Copy
Species: Homo sapiens
Genetic Insert: Aβ(M1-42/C18C33)
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pET vector; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35080857
Proper citation: RRID:Addgene_173759 Copy
Species: Caenorhabditis elegans
Genetic Insert: Prps-0::mKate2::par-6::unc-54 3' UTR
Vector Backbone Description: Backbone Marker:Agilent Technologies; Backbone Size:2999; Vector Backbone:pMLS257; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34849800
Comments: Please visit https://doi.org/10.1101/2021.06.01.446599 for bioRxiv preprint.
Proper citation: RRID:Addgene_173751 Copy
Species: Caenorhabditis elegans
Genetic Insert: Prps-0::mKate2:: par-6(_PB1)::unc-54 3' UTR
Vector Backbone Description: Backbone Marker:Agilent Technologies; Backbone Size:2999; Vector Backbone:pMLS257; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34849800
Comments: Please visit https://doi.org/10.1101/2021.06.01.446599 for bioRxiv preprint.
Proper citation: RRID:Addgene_173752 Copy
Species: Homo sapiens
Genetic Insert: Wnt-3HA
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBS KS(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9419423
Proper citation: RRID:Addgene_17373 Copy
Species: Mus musculus
Genetic Insert: Wnt-3aHA
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBS KS(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9419423
Proper citation: RRID:Addgene_17374 Copy
Species: Mus musculus
Genetic Insert: Wnt-4HA
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBS KS(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9419423
Comments: Expression of multiple novel Wnt-1/int-1-related genes during fetal and adult mouse development.
Gavin BJ, McMahon JA, McMahon AP
In Genes Dev. 1990 Dec . 4(12B):2319-32.
Proper citation: RRID:Addgene_17375 Copy
Species: Other
Genetic Insert: HK022 Integrase
Vector Backbone Description: Backbone Marker:Haldimann and Wanner 2001; Vector Backbone:pAH69; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34190535
Proper citation: RRID:Addgene_173782 Copy
Species: Mus musculus
Genetic Insert: Wnt-6HA
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBS KS(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9419423
Comments: Expression of multiple novel Wnt-1/int-1-related genes during fetal and adult mouse development.
Gavin BJ, McMahon JA, McMahon AP
In Genes Dev. 1990 Dec . 4(12B):2319-32.
Proper citation: RRID:Addgene_17378 Copy
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