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Species: Homo sapiens
Genetic Insert: Flag-hPKL WT
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6819; Vector Backbone:pLHCX; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31825824
Proper citation: RRID:Addgene_107159 Copy
Species: Rattus norvegicus
Genetic Insert: MBP-tagged rat pyruvate kinase liver isoform
Vector Backbone Description: Backbone Size:4467; Vector Backbone:PCRT7; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31825824
Proper citation: RRID:Addgene_107157 Copy
Species: Rattus norvegicus
Genetic Insert: MBP-tagged rat pyruvate kinase liver isoform
Vector Backbone Description: Backbone Size:4467; Vector Backbone:PCRT7; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31825824
Proper citation: RRID:Addgene_107156 Copy
Species: Other
Genetic Insert: TurboID (BirA mutant)
Vector Backbone Description: Vector Backbone:pLX304; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30125270
Comments: Please visit https://www.biorxiv.org/content/early/2017/10/02/196980 for BioRxiv preprint
Proper citation: RRID:Addgene_107175 Copy
Species: Other
Genetic Insert: miniTurbo (BirA mutant)
Vector Backbone Description: Vector Backbone:pCDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30125270
Comments: Please visit https://www.biorxiv.org/content/early/2017/10/02/196980 for BioRxiv preprint
Proper citation: RRID:Addgene_107174 Copy
Species: Homo sapiens
Genetic Insert: p300 delta33
Vector Backbone Description: Backbone Marker:not the same as the commercial CMVb; Backbone Size:4800; Vector Backbone:CMVbeta; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: see author's map for pCMVb p300 wt for map of empty pCMVb vector.
Proper citation: RRID:Addgene_10719 Copy
Species: Homo sapiens
Genetic Insert: MARS
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: See author's map for cloning details. 2 BamHI sites in insert as well.
Proper citation: RRID:Addgene_10716 Copy
Species: Homo sapiens
Genetic Insert: Gamma adaptin 2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: diagnostic digest with BamHI should release 800bp fragment.
Proper citation: RRID:Addgene_10713 Copy
Species: Mus musculus
Genetic Insert: SSeCKs T751A
Vector Backbone Description: Backbone Size:5198; Vector Backbone:pBABE; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29282278
Proper citation: RRID:Addgene_107142 Copy
Species: Homo sapiens
Genetic Insert: HA-tagged Kras4B(G12V) and GFP
Vector Backbone Description: Backbone Size:11261; Vector Backbone:pLVET-IRES-GFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28604746
Comments: K-RasG12V PCR:ed from pLenti-PGK-KRAS4B(G12V)(Addgene #35633) and ligated as PmeI/BamHI fragment into pLVET-IRES-GFP
Proper citation: RRID:Addgene_107140 Copy
Vector Backbone Description: Backbone Marker:Derived from pLVET-tTR-KRAB by P. Aebisher/D. Trono (Addgene plasmid #11644); Backbone Size:11261; Vector Backbone:pLVET; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28604746
Comments: pLVET-GFP-tTR-KRAB vector (Addgene#11644) was modified by first removing tTR-KRAB with EcoRI then ligating IRES-GFP from pMSCV PIG (Addgene #21654) as a blunt end PCR fragment into previously generated pLVET-GFP cut with SmaI. Finally original GFP was removed with EcoRI/MluI => ends blunted and vector religated to generate pLVET-IRES-GFP.
Proper citation: RRID:Addgene_107139 Copy
Genetic Insert: kin-2a(G310D)::SL2::GFP
Vector Backbone Description: Vector Backbone:pHW394; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29581308
Proper citation: RRID:Addgene_107137 Copy
Genetic Insert: NLS::gp41-1-C-intein::cGAL(AD)
Vector Backbone Description: Vector Backbone:pHW393; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29581308
Proper citation: RRID:Addgene_107136 Copy
Genetic Insert: NLS::gp41-1-C-intein::cGAL(AD)
Vector Backbone Description: Vector Backbone:pHW393; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29581308
Proper citation: RRID:Addgene_107135 Copy
Genetic Insert: NLS::cGAL(DBD)::gp41-1-N-intein
Vector Backbone Description: Vector Backbone:pHW393; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29581308
Proper citation: RRID:Addgene_107134 Copy
Genetic Insert: NLS::cGAL(DBD)::gp41-1-N-intein
Vector Backbone Description: Vector Backbone:pHW393; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29581308
Proper citation: RRID:Addgene_107133 Copy
Genetic Insert: NLS::cGAL(DBD)::gp41-1-N-intein
Vector Backbone Description: Vector Backbone:pHW393; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29581308
Proper citation: RRID:Addgene_107132 Copy
Species: Homo sapiens
Genetic Insert: neurofibromin 2
Vector Backbone Description: Backbone Marker:GE Life Sciences; Vector Backbone:pGEX-6P1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29626191
Comments: GST tag in frame with NF2 gene
Note: The NFS insert contains A190V and G302E mutations compared to the closest reference sequence (NP_000259.1). The depositor has confirmed that these do not affect plasmid function.
Proper citation: RRID:Addgene_107149 Copy
Species: Mus musculus
Genetic Insert: Ezh2
Vector Backbone Description: Backbone Size:6573; Vector Backbone:MSCV-IRES-GFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_107146 Copy
Species: Synthetic
Genetic Insert: GFP + sgRNA for GFP
Vector Backbone Description: Vector Backbone:pXPR_047; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA sequence is GGTGAACCGCATCGAGCTGA
pLKO TRC v2 library vector, Puro selection and eGFP expression.
Proper citation: RRID:Addgene_107145 Copy
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