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Species: Homo sapiens
Genetic Insert: CARD8
Vector Backbone Description: Vector Backbone:pDB.His.MBP.3C ; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33420033
Proper citation: RRID:Addgene_164025 Copy
Species: Homo sapiens
Genetic Insert: CARD8
Vector Backbone Description: Vector Backbone:pDB.His.MBP.3C ; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33420033
Proper citation: RRID:Addgene_164026 Copy
Species: Homo sapiens
Genetic Insert: CARD8
Vector Backbone Description: Vector Backbone:pDB.His.MBP.3C ; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33420033
Proper citation: RRID:Addgene_164029 Copy
Species: Homo sapiens
Genetic Insert: ANXA11
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31539493
Proper citation: RRID:Addgene_164203 Copy
Species: Homo sapiens
Genetic Insert: ANXA11
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31539493
Proper citation: RRID:Addgene_164207 Copy
Species: Homo sapiens
Genetic Insert: ANXA11
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31539493
Proper citation: RRID:Addgene_164205 Copy
Species: Homo sapiens
Genetic Insert: ANXA11
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31539493
Comments: Please note: Plasmid contains a S2R mutation in ANAX11. This mutation is not known to affect plasmid function.
Proper citation: RRID:Addgene_164206 Copy
Species: Rattus norvegicus
Genetic Insert: rat Δ188Annexin A11
Vector Backbone Description: Backbone Marker:Gunter Stier; Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32344647
Proper citation: RRID:Addgene_164497 Copy
Species: Rattus norvegicus
Genetic Insert: rat Δ192Annexin A11
Vector Backbone Description: Backbone Marker:Gunter Stier; Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32344647
Proper citation: RRID:Addgene_164498 Copy
Species: Homo sapiens
Genetic Insert: HJURP CenpA Binding Domain
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pET42+b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21412236
Proper citation: RRID:Addgene_30393 Copy
Species: Homo sapiens
Genetic Insert: Ahi1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pDsRed2-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19718039
Comments: Total plasmid size: 8.4kb
Proper citation: RRID:Addgene_30495 Copy
Species: Homo sapiens
Genetic Insert: BCL2-associated X protein
Vector Backbone Description: Backbone Size:4700; Vector Backbone:C3-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21458670
Proper citation: RRID:Addgene_30533 Copy
Species: Bos taurus
Genetic Insert: Mature bovine mitochondrial elongation factor Tu
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5300; Vector Backbone:pET24c(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:8547323
Proper citation: RRID:Addgene_30535 Copy
Species: Escherichia coli
Genetic Insert: beta-glucuronidase
Vector Backbone Description: Backbone Size:2792; Vector Backbone:pBAV1k; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22020504
Comments: In our experience, plasmid yields are highest when cells are harvested at late log stage.
Proper citation: RRID:Addgene_30501 Copy
Species: Escherichia coli
Genetic Insert: beta-glucuronidase
Vector Backbone Description: Backbone Size:2792; Vector Backbone:pBAV1k; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22020504
Comments: In our experience, plasmid yields are highest when cells are harvest at late log stage.
Proper citation: RRID:Addgene_30503 Copy
Species: Escherichia coli
Genetic Insert: beta-glucuronidase
Vector Backbone Description: Backbone Size:2792; Vector Backbone:pBAV1k; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22020504
Comments: In our experience, plasmid yields are highest when cells are harvested at late log stage.
Proper citation: RRID:Addgene_30502 Copy
Species: Homo sapiens
Genetic Insert: alpha 5 integrin
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4300; Vector Backbone:pEGFP-N3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_30450 Copy
Species: Homo sapiens
Genetic Insert: beta2-spectrin
Vector Backbone Description: Backbone Size:4000; Vector Backbone:Modified pEGFP-N1 (see note); Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: This construct DOES NOT contain EGFP- the EGFP has been removed from the pEGFP-N1 backbone.
The following "mutations" have been located in this construct. The Bennett lab believes they are due to incorrect sequence data originally entered into NCBI; however, please be aware of these in case you are designing primers or doing mutagenesis:
Derivations from published sequence:
2.59 c-->t (silent)
4.23 c-->g (H-->D)
5.99 g-->c (R-->T)
6.3 g-->a (silent)
Please refer to the Addgene NGS result for the fully validated sequence.
Proper citation: RRID:Addgene_31070 Copy
Species: Homo sapiens
Genetic Insert: Dynactin-4
Vector Backbone Description: Backbone Size:4000; Vector Backbone:Modified pEGFP-N1 (see note); Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21186323
Comments: For the preparation of HA-dynactin-4, GFP was first cut out of pEGFP-N1 vector between HindIII and NotI, and in its place, we introduced a triple HA sequence that was synthesized by PCR and ligated into the vector. To a recombinant ORF sequence of dynactin-4, we added by PCR a 5′ XhoI site, and a HindIII site on its 3′ end. The dynactin-4 construct was then cloned into the modified 3XHA-containing vector.
Proper citation: RRID:Addgene_31058 Copy
Species: E. coli
Genetic Insert: E. coli elongation factor Ts
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5300; Vector Backbone:pET24c(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:8765000
Proper citation: RRID:Addgene_31173 Copy
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