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Species: Homo sapiens
Genetic Insert: HPRT genomic DNA
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29507284
Proper citation: RRID:Addgene_107277 Copy
Species: Other
Genetic Insert: dSV40-dCas9-10xGCN4
Vector Backbone Description: Vector Backbone:Lentivirus; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29335603
Proper citation: RRID:Addgene_107310 Copy
Species: Synthetic
Genetic Insert: SpCas9-SaCas9 fusion
Vector Backbone Description: Vector Backbone:pCSDest2; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30451839
Proper citation: RRID:Addgene_107319 Copy
Species: Synthetic
Genetic Insert: SpCas9-SaCas9 fusion
Vector Backbone Description: Vector Backbone:pCSDest2; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30451839
Proper citation: RRID:Addgene_107318 Copy
Species: S. aureus
Genetic Insert: SaCas9
Vector Backbone Description: Vector Backbone:pCSDest2; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30451839
Proper citation: RRID:Addgene_107317 Copy
Species: Homo sapiens
Genetic Insert: SNCA-5Asn
Vector Backbone Description: Vector Backbone:pT7-7; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28939194
Proper citation: RRID:Addgene_107295 Copy
Species: Homo sapiens
Genetic Insert: SNCA-5Asp
Vector Backbone Description: Vector Backbone:pT7-7; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28939194
Proper citation: RRID:Addgene_107293 Copy
Species: Synthetic
Genetic Insert: SpCas9-NmCas9 fusion
Vector Backbone Description: Vector Backbone:pCSDest2; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30451839
Proper citation: RRID:Addgene_107326 Copy
Species: Synthetic
Genetic Insert: SpCas9-NmCas9 fusion
Vector Backbone Description: Vector Backbone:pCSDest2; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30451839
Proper citation: RRID:Addgene_107325 Copy
Species: Synthetic
Genetic Insert: SpCas9-NmCas9 fusion
Vector Backbone Description: Vector Backbone:pCSDest2; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30451839
Proper citation: RRID:Addgene_107324 Copy
Species: Synthetic
Genetic Insert: SpCas9-SaCas9 fusion
Vector Backbone Description: Vector Backbone:pCSDest2; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30451839
Proper citation: RRID:Addgene_107322 Copy
Species: Synthetic
Genetic Insert: SpCas9-SaCas9 fusion
Vector Backbone Description: Vector Backbone:pCSDest2; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30451839
Proper citation: RRID:Addgene_107320 Copy
Species: Homo sapiens
Genetic Insert: Interferon gamma receptor 2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3646; Vector Backbone:pMT-BiP-V5-His; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27599734
Comments: Crystal structure of the extracellular part of receptor 2 of human interferon gamma; PDB ID 5eh1; doi: 10.1107/S2059798316012237
Proper citation: RRID:Addgene_107286 Copy
Genetic Insert: VEGFA-TS3 sgRNA
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30451839
Proper citation: RRID:Addgene_107328 Copy
Genetic Insert: RNA motif plasmid cloning backbone
Vector Backbone Description: Vector Backbone:pLEX; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29400715
Comments: Digest plasmid with BsmBI and ligate RNA motif of interest. The RNA motif of interest would be in the 3UTR and flanked by BoxB motifs.
Oligo cloning can be performed similarly to sgRNA cloning. Primers to order:
Primers (5'->3')
F GCTT
Proper citation: RRID:Addgene_107253 Copy
Genetic Insert: HPRT1_B Avr-TALEN (right)
Vector Backbone Description: Vector Backbone:ptCMV-136/63-VR; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29507284
Proper citation: RRID:Addgene_107272 Copy
Genetic Insert: HPRT1_B Avr-TALEN (left)
Vector Backbone Description: Vector Backbone:ptCMV-136/63-VR; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29507284
Proper citation: RRID:Addgene_107271 Copy
Species: Ogataea parapolymorpha
Genetic Insert: polycistronic HH-gRNA-HDV-HH-gRNA-HDV array targetting OpADE2 and NIAD in O. parapolymorpha
Vector Backbone Description: Backbone Marker:Juergens H et al. Genome editing in Kluyveromyces and Ogataea yeasts using a broad-host-range Cas9/gRNA expression plasmid; Backbone Size:10046; Vector Backbone:pUDP002 #103872; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29438517
Proper citation: RRID:Addgene_107269 Copy
Species: Homo sapiens
Genetic Insert: mCherry-eDHFR-Cdc42Q61L
Vector Backbone Description: Backbone Marker:Carmen Williams (Addgene plasmid #32374); Vector Backbone:pIVT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29097549
Proper citation: RRID:Addgene_107267 Copy
Species: E.coli
Genetic Insert: mCherry-eDHFR
Vector Backbone Description: Backbone Marker:Carmen Williams (Addgene plasmid #32374); Vector Backbone:pIVT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29097549
Proper citation: RRID:Addgene_107266 Copy
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