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Species: Synthetic
Genetic Insert: 35S:AtCas9 + AtU6:gRNA
Vector Backbone Description: Vector Backbone:pTRANS_100; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:28522548
Proper citation: RRID:Addgene_91207 Copy
Species: Synthetic
Genetic Insert: nAtCas9_D10A+gRNActrl+donor
Vector Backbone Description: Vector Backbone:pTRANS_201; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28522548
Proper citation: RRID:Addgene_91187 Copy
Species: Synthetic
Genetic Insert: TALENs targeting tomato ANT1
Vector Backbone Description: Vector Backbone:pTC110; Vector Types:TALEN; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28522548
Proper citation: RRID:Addgene_91178 Copy
Species: Synthetic
Genetic Insert: Codon optimized dCas9-Mxi1
Vector Backbone Description: Backbone Size:2623; Vector Backbone:pUC19; Vector Types:Yeast Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28832943
Proper citation: RRID:Addgene_91248 Copy
Species: Synthetic
Genetic Insert: Codon optimized dCas9-Mxi1
Vector Backbone Description: Backbone Size:2623; Vector Backbone:pUC19; Vector Types:Yeast Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28832943
Proper citation: RRID:Addgene_91249 Copy
Species: Synthetic
Genetic Insert: GGG-ELP(40nm)-Cys
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5219; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28591514
Comments: The underlying cloning procedure of the employed ELP genes is described in this article:
Ott, W.; Nicolaus, T.; Gaub, H. E.; Nash, M. A. Sequence-Independent Cloning and Post-Translational Modification of Repetitive Protein Polymers through Sortase and Sfp-Mediated Enzymatic Ligation. Biomacromolecules 2016, 17, 1330−1338.
Proper citation: RRID:Addgene_91571 Copy
Species: Synthetic
Genetic Insert: LoxP-SEC-LoxP
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Worm Expression, Cre/Lox, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32550377
Proper citation: RRID:Addgene_91829 Copy
Species: Synthetic
Genetic Insert: mScarlet-I-C1
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32550377
Proper citation: RRID:Addgene_91826 Copy
Species: Synthetic
Genetic Insert: HaloTag-C1
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32550377
Proper citation: RRID:Addgene_91827 Copy
Species: Synthetic
Genetic Insert: mEGFP-P2A-paAIP2
Vector Backbone Description: Backbone Size:4650; Vector Backbone:pAAV-MCS; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28318784
Proper citation: RRID:Addgene_91718 Copy
Species: Synthetic
Genetic Insert: EGFP
Vector Backbone Description: Backbone Marker:K. Deisseroth Lab (Addgene #37084); Backbone Size:6342; Vector Backbone:pAAV-Ef1a-DIO-EGFP-WPRE-pA; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28888955
Proper citation: RRID:Addgene_91946 Copy
Species: Synthetic
Genetic Insert: Lox511I-SEC-Lox511I
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Worm Expression, Cre/Lox, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32550377
Proper citation: RRID:Addgene_91831 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:F. Zhang Lab (Addgene #60229); Backbone Size:6394; Vector Backbone:AAV:ITR-U6-sgRNA(backbone)-pCBh-Cre-WPRE-hGHpA-ITR; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28888955
Proper citation: RRID:Addgene_91948 Copy
Species: Synthetic
Genetic Insert: C-BLa 2.0 fusion protein
Vector Backbone Description: Backbone Marker:John E. Cronan; Backbone Size:4284; Vector Backbone:pBAD322K (modified); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28432015
Comments: TMD: Integrin alphaV mut GP, contains ApaI restriction site
Proper citation: RRID:Addgene_92060 Copy
Species: Synthetic
Genetic Insert: MTS-mCherry-GFP1-10
Vector Backbone Description: Backbone Marker:PMID: 7737504; Vector Backbone:p404TDH3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28241148
Comments: The MTS sequence is based on subunit 9 of F0-ATP synthase (pOLI1-HcRed) from Curr Biol. 2004 Nov 23;14(22):1996-2004
Proper citation: RRID:Addgene_91958 Copy
Species: Synthetic
Genetic Insert: DsRed-IRES-EGFP
Vector Backbone Description: Backbone Size:8000; Vector Backbone:pLenti-GPS-GAW-IRES; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27779468
Proper citation: RRID:Addgene_92194 Copy
Species: Synthetic
Genetic Insert: deGFP
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:2530; Vector Backbone:pBEST; Vector Types:Bacterial Expression, Synthetic Biology, Cell Free Protein Synthesis (CFPS); Bacterial Resistance:Ampicillin
Defining Citation: PMID:28230975
Comments: Additional reference:
Shin, J., and Noireaux, V. (2010) Efficient cell-free expression with the endogenous E. Coli RNA polymerase and sigma factor 70. J. Biol. Eng. 4, 8.
Proper citation: RRID:Addgene_92224 Copy
Species: Synthetic
Genetic Insert: Sp-dCas9-2xAM tag
Vector Backbone Description: Backbone Marker:Yoichi Sekita; Backbone Size:9921; Vector Backbone:CSII-U6-gRNA-CBh-3xFLAG-PA-dCas9-P2A-Puro; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29482606
Proper citation: RRID:Addgene_92221 Copy
Species: Synthetic
Genetic Insert: deGFP
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:2530; Vector Backbone:pBEST; Vector Types:Bacterial Expression, Synthetic Biology, Cell Free Protein Synthesis (CFPS); Bacterial Resistance:Ampicillin
Defining Citation: PMID:28230975
Comments: Additional reference:
Shin, J., and Noireaux, V. (2010) Efficient cell-free expression with the endogenous E. Coli RNA polymerase and sigma factor 70. J. Biol. Eng. 4, 8.
Proper citation: RRID:Addgene_92222 Copy
Species: Synthetic
Genetic Insert: CaM-V5-TEVp(Δ220-242)
Vector Backbone Description: Backbone Size:3659; Vector Backbone:AAV vector; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28650461
Proper citation: RRID:Addgene_92214 Copy
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