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Species: Homo sapiens
Genetic Insert: mEOS2-YAP1
Vector Backbone Description: Vector Backbone:pBABE (hygro); Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22939869
Comments: Insert size is approximately 3540. The insert is a fusion of mEOS2 and YAP1.
Proper citation: RRID:Addgene_124160 Copy
Species: Homo sapiens
Genetic Insert: M-CSF receptor promoter fragment
Vector Backbone Description: Backbone Size:5900; Vector Backbone:pXP2; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8264604
Proper citation: RRID:Addgene_12420 Copy
Species: Homo sapiens
Genetic Insert: M-CSF receptor promoter fragment
Vector Backbone Description: Backbone Size:5900; Vector Backbone:pXP2; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8622667
Proper citation: RRID:Addgene_12421 Copy
Species: Homo sapiens
Genetic Insert: YAP1
Vector Backbone Description: Vector Backbone:pBABE (hygro); Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22939869
Comments: Insert size is approximately 1580.
Proper citation: RRID:Addgene_124154 Copy
Species: Homo sapiens
Genetic Insert: Minigene containing Her2 sequences fused to endosomal targeting sequence of class I MHC
Vector Backbone Description: Vector Backbone:pSK485; Vector Types:in vitro transcription template; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31043415
Proper citation: RRID:Addgene_124251 Copy
Species: Homo sapiens
Genetic Insert: Minigene containing Her2 sequences fused to endosomal targeting sequence of class I MHC
Vector Backbone Description: Vector Backbone:pSK485; Vector Types:in vitro transcription template; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31043415
Proper citation: RRID:Addgene_124250 Copy
Species: Homo sapiens
Genetic Insert: Minigene containing KRAS sequences fused to endosomal targeting sequence of class I MHC
Vector Backbone Description: Vector Backbone:pSK485; Vector Types:in vitro transcription template; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31043415
Proper citation: RRID:Addgene_124253 Copy
Species: Homo sapiens
Genetic Insert: Minigene containing KRAS sequences fused to endosomal targeting sequence of class I MHC
Vector Backbone Description: Vector Backbone:pSK485; Vector Types:in vitro transcription template; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31043415
Proper citation: RRID:Addgene_124252 Copy
Species: Homo sapiens
Genetic Insert: NGN2, ISL1, LHX3
Vector Backbone Description: Vector Backbone:CLYBL targeting; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_124230 Copy
Species: Homo sapiens
Genetic Insert: UBE2G1
Vector Backbone Description: Vector Backbone:pLCE; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31300418
Proper citation: RRID:Addgene_124299 Copy
Species: Homo sapiens
Genetic Insert: NFATc4 nuclear factor of activated T cells
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid # 62988); Backbone Size:9200; Vector Backbone:pSpCas9(BB)-2A-Puro (PX459) V2.0; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Comments: Plus "G" was routinely added because U6 promoter prefers "G" as the first transcribed nucleotide. gRNA sequence contains at least 3 mismatches, and was selected by choosing the highest scored sequence from different programs and databases.
Proper citation: RRID:Addgene_124290 Copy
Species: Homo sapiens
Genetic Insert: Collagen type VII alpha 1 chain
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid # 62988); Backbone Size:9200; Vector Backbone:pSpCas9(BB)-2A-Puro (PX459) V2.0; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Comments: Plus "G" was routinely added because U6 promoter prefers "G" as the first transcribed nucleotide. gRNA sequence contains at least 3 mismatches, and was selected by choosing the highest scored sequence from different programs and databases.
Proper citation: RRID:Addgene_124286 Copy
Species: Homo sapiens
Genetic Insert: NFATc3 nuclear factor of activated T cells
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid # 62988); Backbone Size:9200; Vector Backbone:pSpCas9(BB)-2A-Puro (PX459) V2.0; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Comments: Plus "G" was routinely added because U6 promoter prefers "G" as the first transcribed nucleotide. gRNA sequence contains at least 3 mismatches, and was selected by choosing the highest scored sequence from different programs and databases.
Proper citation: RRID:Addgene_124288 Copy
Species: Homo sapiens
Genetic Insert: NFATc4 nuclear factor of activated T cells
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid # 62988); Backbone Size:9200; Vector Backbone:pSpCas9(BB)-2A-Puro (PX459) V2.0; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Comments: Plus "G" was routinely added because U6 promoter prefers "G" as the first transcribed nucleotide. gRNA sequence contains at least 3 mismatches, and was selected by choosing the highest scored sequence from different programs and databases.
Proper citation: RRID:Addgene_124289 Copy
Species: Homo sapiens
Genetic Insert: LMNA (R377H)
Vector Backbone Description: Vector Backbone:mRFP-N2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_124270 Copy
Species: Homo sapiens
Genetic Insert: LMNA (R482Q)
Vector Backbone Description: Vector Backbone:mRFP-N2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_124273 Copy
Species: Homo sapiens
Genetic Insert: LMNA (R527P)
Vector Backbone Description: Vector Backbone:mRFP-N2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_124272 Copy
Species: Homo sapiens
Genetic Insert: Halo tag
Vector Backbone Description: Backbone Size:5428; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30358421
Proper citation: RRID:Addgene_124316 Copy
Species: Homo sapiens
Genetic Insert: 68 a.a. fragment of C/EBP alpha
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pGEX-2TK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8622667
Comments: 68 a.a. of human C/EBPa in frame with GST in BamHI and EcoRI sites of pGEX-2TK.
Proper citation: RRID:Addgene_12435 Copy
Species: Homo sapiens
Genetic Insert: AML1-ETO fusion
Vector Backbone Description: Backbone Marker:See comments; Vector Backbone:MigR1; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15569932
Comments: MigR1 is a MSCV-IRES-EGFP retroviral construct, similar to pMig (Addgene vector 9044). See "Pear, Baltimore, Blood. 1998 Nov 15;92(10):3780-92".
Proper citation: RRID:Addgene_12431 Copy
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