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Species: SARS-CoV-2
Genetic Insert: SARS-CoV-2 Receptor Binding domain
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5442; Vector Backbone:pET21b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33532775
Proper citation: RRID:Addgene_167126 Copy
Species: Homo sapiens
Genetic Insert: CSL
Vector Backbone Description: Vector Backbone:pcDNA3.Flag; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31467287
Proper citation: RRID:Addgene_167009 Copy
Species: Homo sapiens
Genetic Insert: PAK5 S573N
Vector Backbone Description: Vector Backbone:p-Lenti-puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29875996
Proper citation: RRID:Addgene_167195 Copy
Species: Homo sapiens
Genetic Insert: PAK5 M173I
Vector Backbone Description: Vector Backbone:p-Lenti-puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29875996
Proper citation: RRID:Addgene_167192 Copy
Species: Homo sapiens
Genetic Insert: PAK5 K478R/479R E294K
Vector Backbone Description: Vector Backbone:p-Lenti-puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29875996
Proper citation: RRID:Addgene_167198 Copy
Species: Synthetic
Genetic Insert: SNAP-tag
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET51b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34339177
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.04.13.439540v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_167269 Copy
Species: Mus musculus
Genetic Insert: Mouse Naked-2 pCS2P+F
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2P+P; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15539487
Comments: Description: Subcloned Keith Wharton pCDNA3 insert into Xba (blunted) and Xho sites of CS2P+
Linearize with Sac2 and transcribe SP6.
Reference: Waxman JS, Hocking AM, Stoick CL, Moon RT.
Zebrafish Dapper1 and Dapper2 play distinct roles in Wnt-mediated developmental processes.
Development. 2004 Dec;131(23):5909-21.
* Addgene's QC finds a 163bp deletion at the 3'end when compared to the NCBI reference sequence
Proper citation: RRID:Addgene_16727 Copy
Species: Xenopus laevis
Genetic Insert: casein kinase 1, epsilon
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15539487
Comments: Description: From John Graff, in Peters et al 1999.
Notes for Use: Select clones with amp.
Reference: used in Waxman JS, Hocking AM, Stoick CL, Moon RT.
Zebrafish Dapper1 and Dapper2 play distinct roles in Wnt-mediated developmental processes.
Development. 2004 Dec;131(23):5909-21.
Proper citation: RRID:Addgene_16725 Copy
Species: Synthetic
Genetic Insert: HaloTag7
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET51b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34339177
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.04.13.439540v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_167267 Copy
Species: Synthetic
Genetic Insert: HaloTag7
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET51b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34339177
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.04.13.439540v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_167266 Copy
Species: SARS-CoV-2
Genetic Insert: Nsp3 (residues 1584-1945)
Vector Backbone Description: Vector Backbone:pMCSG53; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_167261 Copy
Species: SARS-CoV-2
Genetic Insert: Nsp3 (residues 1089-1203)
Vector Backbone Description: Vector Backbone:pMCSG53; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_167260 Copy
Species: SARS-CoV-2
Genetic Insert: Nsp15 (residues 1-346)
Vector Backbone Description: Vector Backbone:pMCSG53; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_167265 Copy
Species: SARS-CoV-2
Genetic Insert: Nsp9 (residues 1-113)
Vector Backbone Description: Vector Backbone:pMCSG53; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_167264 Copy
Species: SARS-CoV-2
Genetic Insert: Nsp3 (residues 413-676)
Vector Backbone Description: Vector Backbone:pMCSG53; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_167258 Copy
Species: SARS-CoV-2
Genetic Insert: Nsp1 (residues 13-127)
Vector Backbone Description: Vector Backbone:pMCSG53; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33319167
Proper citation: RRID:Addgene_167256 Copy
Species: SARS-CoV-2
Genetic Insert: N protein (residues 1-419)
Vector Backbone Description: Vector Backbone:pMCSG53; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_167255 Copy
Species: Homo sapiens
Genetic Insert: MEFV
Vector Backbone Description: Backbone Size:10550; Vector Backbone:pInducer21; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35759198
Proper citation: RRID:Addgene_167019 Copy
Species: Xenopus laevis
Genetic Insert: XCtBP
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2P+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Comments: Plasmid History:
Constructed by Jeff Brown.
Notes for Use:
The ~2 kb dropout of the Sal I digest of XTcf-3 interacting clone 2-1 (in pGBT9) was ligated into the XhoI site of pCS2P+. For CS2+MT XCtBP, the Sal I dropout was blunted with Klenow and cloned into the StuI site of pCS2+MT.
Proper citation: RRID:Addgene_16704 Copy
Species: Homo sapiens
Genetic Insert: PAK5 S364L
Vector Backbone Description: Vector Backbone:pLenti-puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29875996
Proper citation: RRID:Addgene_167168 Copy
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