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Species: Homo sapiens
Genetic Insert: 6x Halo-EGFP- 2x PACT
Vector Backbone Description: Vector Backbone:pERB219; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29097549
Proper citation: RRID:Addgene_107265 Copy
Vector Backbone Description: Backbone Marker:GeneCopoeia; Vector Backbone:pRecLV105; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29431698
Comments: Please visit https://www.biorxiv.org/content/early/2017/02/09/107201 for bioRxiv preprint.
Proper citation: RRID:Addgene_107238 Copy
Genetic Insert: MARK3
Vector Backbone Description: Backbone Marker:Addgene; Vector Backbone:pLX304; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29431698
Comments: Please visit https://www.biorxiv.org/content/early/2017/02/09/107201 for bioRxiv preprint.
Proper citation: RRID:Addgene_107236 Copy
Genetic Insert: MARK3
Vector Backbone Description: Backbone Marker:Addgene; Vector Backbone:pLX304; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29431698
Comments: Please visit https://www.biorxiv.org/content/early/2017/02/09/107201 for bioRxiv preprint.
Proper citation: RRID:Addgene_107235 Copy
Genetic Insert: MEF2C
Vector Backbone Description: Backbone Marker:Stephen Elledge, Thomas Westbrook; Vector Backbone:pINDUCER20; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29431698
Comments: Please visit https://www.biorxiv.org/content/early/2017/02/09/107201 for bioRxiv preprint.
Proper citation: RRID:Addgene_107234 Copy
Genetic Insert: toehold 7 + sfGFP
Vector Backbone Description: Backbone Marker:New England Biolabs (cloning vector); Backbone Size:2202; Vector Backbone:pNP1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30083608
Proper citation: RRID:Addgene_107355 Copy
Species: Homo sapiens
Genetic Insert: MARK3 shRNA (22 nt)
Vector Backbone Description: Backbone Marker:Scott Lowe; Vector Backbone:pLT3REVIR; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29431698
Comments: Please visit https://www.biorxiv.org/content/early/2017/02/09/107201 for bioRxiv preprint.
Proper citation: RRID:Addgene_107233 Copy
Species: Homo sapiens
Genetic Insert: MEF2C
Vector Backbone Description: Backbone Marker:Scott Armstrong; Vector Backbone:MSCV-IRES-Tomato; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29431698
Comments: Please visit https://www.biorxiv.org/content/early/2017/02/09/107201 for bioRxiv preprint.
Proper citation: RRID:Addgene_107231 Copy
Genetic Insert: MEF2C S222A
Vector Backbone Description: Backbone Marker:Eric Still; Vector Backbone:pRecLV105; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29431698
Comments: Please visit https://www.biorxiv.org/content/early/2017/02/09/107201 for bioRxiv preprint.
Proper citation: RRID:Addgene_107239 Copy
Species: Synthetic
Genetic Insert: BASU
Vector Backbone Description: Vector Backbone:pLEX; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29400715
Comments: The BASU protein sequence has 4 mutations relative to wild type Bacillus subtilis biotin ligase (Uniprot P0CI75): N terminal truncation of 65 aa, R124G, E323S and G325R.
Proper citation: RRID:Addgene_107250 Copy
Species: Synthetic
Genetic Insert: hM3DREADD
Vector Backbone Description: Backbone Size:6597; Vector Backbone:AAV2; Vector Types:AAV; Bacterial Resistance:Ampicillin
Comments: Please visit https://www.biorxiv.org/content/early/2018/01/22/251769 for bioRxiv preprint.
These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community.
Proper citation: RRID:Addgene_107246 Copy
Species: Synthetic
Genetic Insert: Rncp-iGluSnFR1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5268; Vector Backbone:pDisplay; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29308878
Comments: Please note that mutation M178K in the Neomycin resistance cassette was found during Addgene's quality control. Neomycin selection has not been tested for this plasmid, but should not affect sensor function.
Proper citation: RRID:Addgene_107336 Copy
Species: Homo sapiens
Genetic Insert: SNCA-9Asn
Vector Backbone Description: Vector Backbone:pT7-7; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28939194
Proper citation: RRID:Addgene_107297 Copy
Genetic Insert: VEGFA-TS3 sgRNA
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30451839
Proper citation: RRID:Addgene_107330 Copy
Species: Homo sapiens
Genetic Insert: RB del663
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pSG5L; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9420334
Comments: del663 created by site-directed mutagenesis using this primer: GCCTATCTCCGGCTAaatgctgctatacgatcgCTTCTGTCTGAGCAC
Proper citation: RRID:Addgene_10728 Copy
Species: Homo sapiens
Genetic Insert: RB 567L
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pSG5L; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9420334
Comments: 567L created by site-directed mutagenesis using this primer: GCATGGCTCTCAGATcttCCTTTATTTGATCTT
Proper citation: RRID:Addgene_10725 Copy
Species: Homo sapiens
Genetic Insert: BirA-UBE3B
Vector Backbone Description: Backbone Size:8247; Vector Backbone:pLVX-GWB-IRES-Puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28003368
Proper citation: RRID:Addgene_107351 Copy
Species: Homo sapiens
Genetic Insert: UBE3BdelIQ
Vector Backbone Description: Backbone Size:8247; Vector Backbone:pLVX-GWB-IRES-Puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28003368
Proper citation: RRID:Addgene_107350 Copy
Species: Homo sapiens
Genetic Insert: UBE3B(C1036A)
Vector Backbone Description: Backbone Size:8247; Vector Backbone:pLVX-GWB-IRES-Puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28003368
Proper citation: RRID:Addgene_107348 Copy
Species: Homo sapiens
Genetic Insert: UBE3BΔHECT
Vector Backbone Description: Backbone Size:8247; Vector Backbone:pLVX-GWB-IRES-Puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28003368
Proper citation: RRID:Addgene_107347 Copy
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