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Genetic Insert: Control (“C”) lacking sg-1
Vector Backbone Description: Vector Backbone:pCOLA; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32488086
Comments: GenBank file with original annotations can be downloaded from the "Resource Information" section. Please note that, in addition, plasmid pJ1996_v2 is needed for the circuit encoded on this plasmid to operate as designed.
Proper citation: RRID:Addgene_140689 Copy
Species: Synthetic
Genetic Insert: sfCFP
Vector Backbone Description: Backbone Size:3231; Vector Backbone:pMB1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31361464
Proper citation: RRID:Addgene_140695 Copy
Species: Mus musculus
Genetic Insert: PI3K regulatory subunit p85beta
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Cantley lab recommends growth at room temperature.
Proper citation: RRID:Addgene_1408 Copy
Species: Synthetic
Genetic Insert: PuroTK
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pDONR; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31454364
Comments: Plate note: Plasmid contains a single G nucleotide deletion in the attL1 sequence. This mutation is not known to affect plasmid function.
Proper citation: RRID:Addgene_141005 Copy
Species: Synthetic
Genetic Insert: HygTK
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pDONR; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31454364
Proper citation: RRID:Addgene_141006 Copy
Species: Escherichia coli
Genetic Insert: Neomycin resistance gene
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pDONR; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31454364
Proper citation: RRID:Addgene_141007 Copy
Species: Synthetic
Genetic Insert: TKNeo
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pDONR; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31454364
Proper citation: RRID:Addgene_141008 Copy
Species: Synthetic
Genetic Insert: TKBsd
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pDONR; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31454364
Proper citation: RRID:Addgene_141009 Copy
Species: phage T3
Genetic Insert: T3 RNA polymerase
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pSC101; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30487584
Proper citation: RRID:Addgene_140872 Copy
Species: Synthetic
Genetic Insert: mScarlet
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR-221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32709891
Proper citation: RRID:Addgene_140874 Copy
Species: Homo sapiens
Genetic Insert: Human RhoC
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3519; Vector Backbone:PCR-BluntII-TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:10952316
Proper citation: RRID:Addgene_14099 Copy
Vector Backbone Description: Backbone Marker:pUA66 was described in Zaslaver et al. (2006) Nat. Methods 3: 623-628.; Backbone Size:4849; Vector Backbone:Derived from pUA66; Vector Types:Bacterial Expression, Vector for the I-Block assay designed to study DNA-protein interaction in E. coli.; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31980824
Comments: Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_141079 Copy
Species: Homo sapiens
Genetic Insert: GCN5
Vector Backbone Description: Backbone Size:25000; Vector Backbone:pAdEasy; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16753578
Comments: Flag-GCN5 was cloned into the pAdTrack shuttle vector and adenovirus constructs were created by recombination of the shuttle vector and pAdEasy vector by electroporation into BJ5183-AD-1 bacteria (Stratagene).
AdEasy is a registered trademark of the Johns Hopkins University.
Proper citation: RRID:Addgene_14106 Copy
Species: Homo sapiens
Genetic Insert: NFAT5
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12080349
Comments: DNA binding domain mutant, dominant negative mutant. Also called DBD5.
Proper citation: RRID:Addgene_14112 Copy
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pDONR; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31454364
Proper citation: RRID:Addgene_141017 Copy
Species: Synthetic
Genetic Insert: mVenus
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pDONR; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31454364
Comments: Please note: Plasmid contains a K206A mutation in Venus. This mutation is not known to affect plasmid function.
Proper citation: RRID:Addgene_141019 Copy
Species: Synthetic
Genetic Insert: EGFP
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pDONR; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31454364
Proper citation: RRID:Addgene_141024 Copy
Species: Synthetic
Genetic Insert: mCerulean
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pDONR; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31454364
Proper citation: RRID:Addgene_141020 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pDONR; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31454364
Proper citation: RRID:Addgene_141022 Copy
Species: Human cytomegalovirus
Genetic Insert: CMV promoter
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pDONR; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31454364
Proper citation: RRID:Addgene_141035 Copy
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