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Species: Synthetic
Genetic Insert: ATeam1.03 codon optimized for yeast (Saccharomyces cerevisiae)
Vector Backbone Description: Backbone Marker:Voth et al. (2001) Nucleic Acids Res (PMID: 11410682); Backbone Size:6062; Vector Backbone:HO-poly-KanMX4-HO; Vector Types:Yeast Expression, Integration and expression into yeast.; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27989441
Comments: The original version of the ATeam1.03 FRET sensor (tested in mammalian cells but not codon optimized for yeast) was published in 2009 by Imamura et al (PMID: 19720993)
Proper citation: RRID:Addgene_92179 Copy
Species: Synthetic
Genetic Insert: NRX-TM-Nav1.6 -CaMbp(M2)-eLOV-TEVcs(ENLYFQ▲M)-tTA-VP16
Vector Backbone Description: Backbone Size:3659; Vector Backbone:AAV vector; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28650461
Proper citation: RRID:Addgene_92213 Copy
Species: Synthetic
Genetic Insert: i53
Vector Backbone Description: Backbone Marker:Agilent; Backbone Size:4614; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29176614
Comments: bioRxiv (10.1101/060954)
Proper citation: RRID:Addgene_92170 Copy
Species: Synthetic
Genetic Insert: TALEN
Vector Backbone Description: Backbone Size:5819; Vector Backbone:pCAG-ZEO-IRES; Vector Types:TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30811996
Proper citation: RRID:Addgene_92245 Copy
Species: Synthetic
Genetic Insert: TALEN
Vector Backbone Description: Backbone Size:5843; Vector Backbone:pCAG-BSD-IRES; Vector Types:TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30811996
Proper citation: RRID:Addgene_92246 Copy
Species: Synthetic
Genetic Insert: TALEN
Vector Backbone Description: Backbone Size:5055; Vector Backbone:pcDNA4/myc-hisA; Vector Types:TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30811996
Proper citation: RRID:Addgene_92243 Copy
Species: Synthetic
Genetic Insert: Neuron-astrocyte proximity assay - Neuronal
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29621490
Proper citation: RRID:Addgene_92278 Copy
Species: Synthetic
Genetic Insert: Ancestral AAV Capsid Anc80L65AAP
Vector Backbone Description: Backbone Size:5238; Vector Backbone:synthetic; Vector Types:Mammalian Expression, AAV, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28165475
Proper citation: RRID:Addgene_92307 Copy
Species: Synthetic
Genetic Insert: Neuron-astrocyte proximity assay - Neuronal
Vector Backbone Description: Vector Backbone:pZac2.1; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29621490
Proper citation: RRID:Addgene_92283 Copy
Species: Synthetic
Genetic Insert: Neuron-astrocyte proximity assay - Astrocyte
Vector Backbone Description: Vector Backbone:pZac2.1; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29621490
Proper citation: RRID:Addgene_92281 Copy
Species: Synthetic
Genetic Insert: pZ-YFP
Vector Backbone Description: Backbone Size:6700; Vector Backbone:pNH603; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27930885
Proper citation: RRID:Addgene_90409 Copy
Species: Synthetic
Genetic Insert: TagGFP2-TEV-GGG-ELP(120nm)-Cys
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5219; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: The underlying cloning procedure of the employed ELP genes is described in this article:
Ott, W.; Nicolaus, T.; Gaub, H. E.; Nash, M. A. Sequence-Independent Cloning and Post-Translational Modification of Repetitive Protein Polymers through Sortase and Sfp-Mediated Enzymatic Ligation. Biomacromolecules 2016, 17, 1330−1338.
Proper citation: RRID:Addgene_90473 Copy
Species: Synthetic
Genetic Insert: Cys-ELP(120nm)-LPETGG
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5216; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28591514
Comments: The underlying cloning procedure of the employed ELP genes is described in this article:
Ott, W.; Nicolaus, T.; Gaub, H. E.; Nash, M. A. Sequence-Independent Cloning and Post-Translational Modification of Repetitive Protein Polymers through Sortase and Sfp-Mediated Enzymatic Ligation. Biomacromolecules 2016, 17, 1330−1338.
Proper citation: RRID:Addgene_90472 Copy
Species: Synthetic
Genetic Insert: TagGFP2-TEV-GGG-ELP(40nm)-Cys
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5216; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: The underlying cloning procedure of the employed ELP genes is described in this article:
Ott, W.; Nicolaus, T.; Gaub, H. E.; Nash, M. A. Sequence-Independent Cloning and Post-Translational Modification of Repetitive Protein Polymers through Sortase and Sfp-Mediated Enzymatic Ligation. Biomacromolecules 2016, 17, 1330−1338.
Proper citation: RRID:Addgene_90474 Copy
Species: Synthetic
Genetic Insert: TALEN_1 backbone with Esp3I ccdb cassette for repeat cloning
Vector Backbone Description: Vector Backbone:pMOD_A1001; Vector Types:TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28522548
Proper citation: RRID:Addgene_91029 Copy
Species: Synthetic
Genetic Insert: Csy4-P2A-AtCas9_dead (D10A + H840A)
Vector Backbone Description: Vector Backbone:pMOD_A1001; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28522548
Proper citation: RRID:Addgene_91024 Copy
Species: Synthetic
Genetic Insert: Csy4-P2A-AtCas9_dead (D10A + H840A)
Vector Backbone Description: Vector Backbone:pMOD_A1001; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28522548
Proper citation: RRID:Addgene_91023 Copy
Species: Synthetic
Genetic Insert: AtCas9_dead (D10A + H840A)
Vector Backbone Description: Vector Backbone:pMOD_A1001; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28522548
Proper citation: RRID:Addgene_91010 Copy
Species: Synthetic
Genetic Insert: TALE-VP64_1 backbone with Esp3I ccdb cassette for repeat cloning
Vector Backbone Description: Vector Backbone:pMOD_A1001; Vector Types:TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28522548
Proper citation: RRID:Addgene_91049 Copy
Species: Synthetic
Genetic Insert: TaCas9_D10A nickase
Vector Backbone Description: Vector Backbone:pMOD_A1001; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28522548
Proper citation: RRID:Addgene_91033 Copy
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