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Species: Solanum lycopersicum M82
Genetic Insert: ID#25
Vector Backbone Description: Vector Backbone:pDONOR207; Vector Types:Gateway Cloning; Bacterial Resistance:Gentamicin
Defining Citation: PMID:37248633
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.08.23.457316v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_175909 Copy
Species: Homo sapiens
Genetic Insert: K2P10.1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2682; Vector Backbone:pACEBAC1; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:35421309
Proper citation: RRID:Addgene_191475 Copy
Genetic Insert: Chimeric Tail Fiber Fusion P2-gpH and S16 gp37
Vector Backbone Description: Vector Backbone:PAJ694; Vector Types:Bacterial Expression, Unspecified; Bacterial Resistance:Gentamicin
Defining Citation: PMID:36084285
Proper citation: RRID:Addgene_178947 Copy
Species: Solanum lycopersicum M82
Genetic Insert: ID#13
Vector Backbone Description: Vector Backbone:pDONOR207; Vector Types:Gateway Cloning; Bacterial Resistance:Gentamicin
Defining Citation: PMID:37248633
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.08.23.457316v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_175897 Copy
Vector Backbone Description: Backbone Marker:Strand et al., 2014; Backbone Size:56450; Vector Backbone:pTA-Mob; Vector Types:Bacterial Expression, Synthetic Biology, Conjugation helper plasmid; Bacterial Resistance:Gentamicin
Defining Citation: PMID:31640164
Proper citation: RRID:Addgene_149662 Copy
Genetic Insert: mRuby2
Vector Backbone Description: Vector Backbone:BBR1-GmR-pAMβ1-EryR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Gentamicin
Proper citation: RRID:Addgene_191419 Copy
Genetic Insert: mRuby2
Vector Backbone Description: Vector Backbone:pProUSER63H0G; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Gentamicin
Proper citation: RRID:Addgene_191421 Copy
Species: Tetrahymena thermophila
Genetic Insert: Q22MS1
Vector Backbone Description: Vector Backbone:pACEBac1; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:33632841
Proper citation: RRID:Addgene_170314 Copy
Species: Tetrahymena thermophila
Genetic Insert: Q22YU3
Vector Backbone Description: Vector Backbone:pACEBac1; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:33632841
Comments: Addgene QC sequencing identified an A707V discrepancy relative to XP_001010823.2. The depositing laboratory has confirmed that this should not affect function.
Proper citation: RRID:Addgene_170315 Copy
Species: Aspergillus nidulans
Genetic Insert: Rhamnogalacturonan lyase A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5585; Vector Backbone:pDONR207; Vector Types:Gateway Donor vector / entry clone; Bacterial Resistance:Gentamicin
Defining Citation: PMID:34059917
Comments: For use with Three-way Multi-site Gateway Cloning (Invitrogen).
Proper citation: RRID:Addgene_170720 Copy
Species: Synthetic
Genetic Insert: I-SceI_sacB
Vector Backbone Description: Backbone Marker:Kenneth M. Peterson; Backbone Size:4768; Vector Backbone:pBBR1-MCS5; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:33930546
Comments: Alternative names: pBBR1-GmR_I-SceI_sacB
Proper citation: RRID:Addgene_171155 Copy
Species: Synthetic
Genetic Insert: J306 scRNA
Vector Backbone Description: Backbone Marker:Kenneth M. Peterson; Backbone Size:4768; Vector Backbone:pBBR1-MCS5; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Gentamicin
Defining Citation: PMID:33930546
Comments: Alternative names: pCK120.306, pBBR1-GmR_J3-BBa_J23117-mRFP_J306
Proper citation: RRID:Addgene_171148 Copy
Species: Synthetic
Genetic Insert: hAAVS1 scRNA
Vector Backbone Description: Backbone Marker:Jesse Zalatan; Backbone Size:4568; Vector Backbone:pRK2-GmR; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Gentamicin
Defining Citation: PMID:33930546
Comments: Alternative names: pCK079, pRK2-GmR_J1-BBa_J23117-mRFP_hAAVS1
Proper citation: RRID:Addgene_171146 Copy
Species: Synthetic
Genetic Insert: J109 scRNA
Vector Backbone Description: Backbone Marker:Kenneth M. Peterson; Backbone Size:4768; Vector Backbone:pBBR1-MCS5; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Gentamicin
Defining Citation: PMID:33930546
Comments: Alternative names: pCK057, pBBR1-GmR_J1-mRFP_J109
Proper citation: RRID:Addgene_171144 Copy
Species: Synthetic
Genetic Insert: J106 scRNA
Vector Backbone Description: Backbone Marker:Kenneth M. Peterson; Backbone Size:4768; Vector Backbone:pBBR1-MCS5; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Gentamicin
Defining Citation: PMID:33930546
Comments: Alternative names: pCDP023.6, pBBR1-GmR_J106
Proper citation: RRID:Addgene_171141 Copy
Species: P. putida
Genetic Insert: XylS-Pm
Vector Backbone Description: Backbone Marker:Jesse Zalatan; Backbone Size:10153; Vector Backbone:pPPC001; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Gentamicin
Defining Citation: PMID:33930546
Comments: Alternative names: pCK193, pUC18TminiTn7T-Gm_XylS-Pm-dCas9_BBa_J23107-MCP-SoxS(R93A/S101A)
Used for conjugation, with pTNS1 and pRK2013 helper plasmids, to integrate XylS-Pm-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) cassettes into gram-negative bacteria.
Point mutation in the FRT site was observed and remained applicable with counterselection by pCK255
Proper citation: RRID:Addgene_171140 Copy
Species: S. pyogenes (dCas9), MS2 (MCP), E. coli (SoxS)
Genetic Insert: Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
Vector Backbone Description: Backbone Marker:Herbert Schweizer; Backbone Size:4569; Vector Backbone:pUC18TminiTn7T-Gm; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Gentamicin
Defining Citation: PMID:33930546
Comments: Alternative names: pCDP041, pUC18TminiTn7T-Gm_Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A/S101A)
Used for conjugation, with pTNS1 and pRK2013 helper plasmids, to integrate Sp.pCas9-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) cassettes into gram-negative bacteria.
Point mutation in the FRT site was observed and remained applicable with counterselection by pCK255
Proper citation: RRID:Addgene_171138 Copy
Species: Homo sapiens
Genetic Insert: SMAD4
Vector Backbone Description: Backbone Marker:Addgene #83274; Backbone Size:4422; Vector Backbone:pEN_TTmiRc2; Vector Types:Gateway Cloning; Bacterial Resistance:Gentamicin
Defining Citation: PMID:29161592
Comments: Gateway entry vector for expression of 3xFLAG SMAD4
Proper citation: RRID:Addgene_83094 Copy
Species: Synechoccus elongatus PCC 7942
Genetic Insert: PkaiB-KaiC-ECFP
Vector Backbone Description: Vector Backbone:PkaiB-kaiC-ecfp expressed from NS2 vector with Gm resistance constructed by seamless assembly.; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:25127213
Proper citation: RRID:Addgene_85121 Copy
Species: Synthetic
Genetic Insert: C321.∆A.opt strain
Vector Backbone Description: Vector Backbone:Whole genome; Vector Types:Synthetic Biology; Bacterial Resistance:Gentamicin
Defining Citation: PMID:28545477
Comments: C321.∆A.opt is a variant of C321.∆A (Addgene Item #48998) engineered to have a faster growth rate, as reported in Kuznetsov, Gleb, et al. "Optimizing complex phenotypes through model-guided multiplex genome engineering." bioRxiv (2016): 086595.
This strain has a heat-inducible lambda prophage (42 C) and should be grown at or below 34 C to avoid induction.
The original strain C321.∆A (Addgene Item #48998) was reported in Lajoie, Marc J., et al. "Genomically recoded organisms expand biological functions." Science 342.6156 (2013): 357-360.
Proper citation: RRID:Addgene_87359 Copy
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