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Species: Arabidopsis thaliana
Genetic Insert: BsaI:GGAG:pAG:AATG:BsaI
Vector Backbone Description: Backbone Size:2247; Vector Backbone:pICH41295; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30625150
Comments: pICSL12022 carries GGAG-TACT golden gate ends. It has to be cloned with a 5’UTR plasmid, TACT-AATG, when assembled with a CDS and terminator into Level 1. For instance pICH41402 (plasmid 50285 on Addgene) can be used. This assembly of promoter / 5’UTR / CDS / terminator can be done in a single reaction.
Cloned by Laurence Tomlinson . Please visit https://www.biorxiv.org/content/early/2018/09/17/419952 for bioRxiv preprint.
Proper citation: RRID:Addgene_117510 Copy
Species: Arabidopsis thaliana
Genetic Insert: BsaI:GGAG:pRPS5a:AATG:BsaI
Vector Backbone Description: Backbone Size:2247; Vector Backbone:pICH41295; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30625150
Comments: Cloned by Baptiste Castel . Please visit https://www.biorxiv.org/content/early/2018/09/17/419952 for bioRxiv preprint.
Proper citation: RRID:Addgene_117512 Copy
Species: Arabidopsis thaliana
Genetic Insert: BsaI:GGAG:pICU2:AATG:BsaI
Vector Backbone Description: Backbone Size:2247; Vector Backbone:pICH41295; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30625150
Comments: Cloned by Laurence Tomlinson . Please visit https://www.biorxiv.org/content/early/2018/09/17/419952 for bioRxiv preprint.
Proper citation: RRID:Addgene_117511 Copy
Species: Pisum sativum
Genetic Insert: BsaI:GCTT:E9t:CGCT:BsaI
Vector Backbone Description: Backbone Size:2247; Vector Backbone:pICH41276; Vector Types:; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30625150
Comments: Cloned by Laurence Tomlinson . Please visit https://www.biorxiv.org/content/early/2018/09/17/419952 for bioRxiv preprint.
Proper citation: RRID:Addgene_117519 Copy
Species: Synthetic
Genetic Insert: BsaI:AATG:Cas9_2:GCTT:BsaI
Vector Backbone Description: Backbone Size:2247; Vector Backbone:pICH41308; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30625150
Comments: Cloned by Oleg Raitskin . Please visit https://www.biorxiv.org/content/early/2018/09/17/419952 for bioRxiv preprint.
Proper citation: RRID:Addgene_117514 Copy
Species: Arabidopsis thaliana
Genetic Insert: BsaI:GGAG:pMGE1:AATG:BsaI
Vector Backbone Description: Backbone Size:2247; Vector Backbone:pICH41295; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30625150
Comments: Cloned by Baptiste Castel . Please visit https://www.biorxiv.org/content/early/2018/09/17/419952 for bioRxiv preprint.
Proper citation: RRID:Addgene_117509 Copy
Species: Staphylococcus aureus
Genetic Insert: SaCas9 (with stop codon)
Vector Backbone Description: Vector Backbone:pICH41308; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30811422
Comments: Compatible with MoClo, GB, Loop Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint
Proper citation: RRID:Addgene_117531 Copy
Species: Lachnospiraceae bacterium ND2006
Genetic Insert: LbCas12a (with stop codon)
Vector Backbone Description: Vector Backbone:pICH41308; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30811422
Comments: Compatible with MoClo, GB Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint
Proper citation: RRID:Addgene_117534 Copy
Species: Staphylococcus aureus
Genetic Insert: eSaCas9 (no stop codon)
Vector Backbone Description: Vector Backbone:pAGM1287; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30811422
Comments: Compatible with MoClo, GB, Loop Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint
Proper citation: RRID:Addgene_117533 Copy
Species: Aequorea victoria
Genetic Insert: c-term tag YFP
Vector Backbone Description: Vector Backbone:pICSL01003; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30811422
Comments: Compatible with MoClo, GB, Loop Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint
Proper citation: RRID:Addgene_117536 Copy
Species: Arabidopsis thaliana
Genetic Insert: CTP-mCitrine
Vector Backbone Description: Backbone Size:9204; Vector Backbone:pN_35S; Vector Types:Plant Expression, Plant/bacteria binary vector; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30884945
Comments: mCitrine contains an L7E compared to the published mCitrine sequence that does not impact plasmid function.
Proper citation: RRID:Addgene_117989 Copy
Species: Fusarium oxysporum f.sp lycopersici
Genetic Insert: dspSix5
Vector Backbone Description: Vector Backbone:pGWB402; Vector Types:Bacterial Expression, Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29481865
Comments: Original backbone received from Tsuyoshi Nakagawa, Shimane University. Described in: https://doi.org/10.1271/bbb.70216 (Nakagawa et al, 2007)
Proper citation: RRID:Addgene_118013 Copy
Genetic Insert: GFPmut2
Vector Backbone Description: Vector Backbone:pGB2; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31142623
Proper citation: RRID:Addgene_118280 Copy
Vector Backbone Description: Vector Backbone:pYPQ141-ZmUbi-RZ-Fn; Vector Types:Plant Expression, CRISPR, Gateway compatible crRNA entry clone; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29567452
Proper citation: RRID:Addgene_108864 Copy
Species: Francisella tularensis subsp. novicida
Genetic Insert: FnCpf1
Vector Backbone Description: Vector Backbone:pYPQ167; Vector Types:Plant Expression, CRISPR, Gateway compatible Cpf1 entry clone; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29567452
Comments: Cpf1 is rice codon optimized
Proper citation: RRID:Addgene_108859 Copy
Species: Homo sapiens
Genetic Insert: human full-length REST/NRSF
Vector Backbone Description: Backbone Marker:Geneva Biotech; Vector Backbone:pIDS; Vector Types:Insect Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:28218430
Proper citation: RRID:Addgene_109162 Copy
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29553576
Proper citation: RRID:Addgene_109234 Copy
Species: Synthetic
Genetic Insert: sfGFP
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29553576
Proper citation: RRID:Addgene_109237 Copy
Species: Synthetic
Genetic Insert: Tetracycline resistance reporter of ChnR activation
Vector Backbone Description: Vector Backbone:pSC101; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29371642
Comments: lactam-biosensing pMGT1 tetA reporter plasmid, pSC101 Ori
Proper citation: RRID:Addgene_109404 Copy
Species: Arabidopsis thaliana
Genetic Insert: Csy4
Vector Backbone Description: Vector Backbone:pTKan-GW-R1R2; Vector Types:Bacterial Expression, Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:28094975
Proper citation: RRID:Addgene_110145 Copy
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