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Species: Caenorhabditis elegans
Genetic Insert: smo-1
Vector Backbone Description: Backbone Size:5365; Vector Backbone:pHISTEV30a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25475837
Proper citation: RRID:Addgene_87054 Copy
Species: Caenorhabditis elegans
Genetic Insert: UBC-9
Vector Backbone Description: Backbone Size:5365; Vector Backbone:PpHISTEV30a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25475837
Proper citation: RRID:Addgene_87055 Copy
Species: Caenorhabditis elegans
Genetic Insert: gtbp‐1 (flanking sequence)::eGFP::gtbp‐1 (27bp)::PH::gtbp‐ 1 (flanking sequence)
Vector Backbone Description: Backbone Size:3200; Vector Backbone:pUC19; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27257074
Proper citation: RRID:Addgene_87247 Copy
Species: Caenorhabditis elegans
Genetic Insert: TRIC-B1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3221; Vector Backbone:pPICZ A; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:27698420
Comments: Vector pPICZ A and insert sequence were modified as follows:
1. Restriction sites including Pml I; Sfi I; BsmB I; Asp718 I; Kpn I were destroyed during cloning full-length CeTRIC-B1 to the vector.
2. In the C-terminal truncation, the sequence between XhoI and polyhistidine had been deleted. That is to say, restriction sites including Xho I, Sac II, Not I, Apa I, and myc-epitope, were destroyed during truncation.
3. A kozak suqence "ACC" was inserted between the restriction site "EcoR I" and initial codon, we added these 3 bp into insert sequence size.
In summary, the size of pPICZ A backbone is changed from 3329 bp to be 3221 bp.
Proper citation: RRID:Addgene_87344 Copy
Species: Caenorhabditis elegans
Genetic Insert: Punc-17b-PH-miniSOG(Q103L)
Vector Backbone Description: Backbone Size:3000; Vector Backbone:Gateway Final LR Clone; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26861262
Proper citation: RRID:Addgene_87761 Copy
Species: Caenorhabditis elegans
Genetic Insert: Pnmr-1-PH-miniSOG
Vector Backbone Description: Backbone Size:2900; Vector Backbone:Gateway Final LR Clone; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26861262
Proper citation: RRID:Addgene_87763 Copy
Species: Caenorhabditis elegans
Genetic Insert: Pcol-19-PH-miniSOG(Q103L)
Vector Backbone Description: Backbone Size:2900; Vector Backbone:Gateway Final LR Clone; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26861262
Proper citation: RRID:Addgene_87758 Copy
Species: Caenorhabditis elegans
Genetic Insert: sls-1.2
Vector Backbone Description: Backbone Marker:Transgen; Backbone Size:3830; Vector Backbone:pEASY Blunt Simple Cloning Kit; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27557708
Proper citation: RRID:Addgene_79003 Copy
Species: Caenorhabditis elegans
Genetic Insert: sls-1.2
Vector Backbone Description: Backbone Marker:Transgen; Backbone Size:3830; Vector Backbone:pEASY Blunt Simple Cloning Kit; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27557708
Proper citation: RRID:Addgene_79002 Copy
Species: Caenorhabditis elegans
Genetic Insert: Cas9 and sgRNA against unc-73 exon2
Vector Backbone Description: Backbone Marker:Bob Goldstein (Addgene plasmid # 47549); Vector Backbone:pDD162; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27274054
Proper citation: RRID:Addgene_82359 Copy
Species: Caenorhabditis elegans
Genetic Insert: unc-73 b isoform
Vector Backbone Description: Vector Backbone:pDONR-221; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27274054
Proper citation: RRID:Addgene_82362 Copy
Species: Caenorhabditis elegans
Genetic Insert: Pmex-5:GFP::H2B::PEST::set-2 3'UTR
Vector Backbone Description: Vector Backbone:pCFJ150; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27294288
Proper citation: RRID:Addgene_82546 Copy
Species: Caenorhabditis elegans
Genetic Insert: oma-1 ORF
Vector Backbone Description: Vector Backbone:L4440; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24014033
Proper citation: RRID:Addgene_82547 Copy
Species: Caenorhabditis elegans
Genetic Insert: oma-2 ORF
Vector Backbone Description: Vector Backbone:L4440; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24014033
Proper citation: RRID:Addgene_82548 Copy
Species: Caenorhabditis elegans
Genetic Insert: Pmex-5:GFP::H2B::PEST::lin-26 3'UTR
Vector Backbone Description: Vector Backbone:pCFJ150; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27294288
Proper citation: RRID:Addgene_80357 Copy
Species: Caenorhabditis elegans
Genetic Insert: Pmex-5:GFP::H2B::PEST::ets-4 3'UTR
Vector Backbone Description: Vector Backbone:pCFJ150; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27294288
Proper citation: RRID:Addgene_80355 Copy
Species: Caenorhabditis elegans
Genetic Insert: Pmex-5:GFP::H2B::PEST::cul-4 3'UTR
Vector Backbone Description: Vector Backbone:pCFJ150; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27294288
Proper citation: RRID:Addgene_80353 Copy
Species: Caenorhabditis elegans
Genetic Insert: Pmex-5:GFP::H2B::PEST::atg4.2 3'UTR
Vector Backbone Description: Vector Backbone:pCFJ150; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27294288
Proper citation: RRID:Addgene_80352 Copy
Species: Caenorhabditis elegans
Genetic Insert: sls-1.2
Vector Backbone Description: Backbone Marker:Transgen; Backbone Size:3830; Vector Backbone:pEASY-Blunt Simple Cloning Vector; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:27557708
Proper citation: RRID:Addgene_81114 Copy
Species: Caenorhabditis elegans
Genetic Insert: Pmyo-2::GFP
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32550377
Comments: The Pmyo-2::GFP cassette is inserted in place of a fluorescent protein in the assembled repair template. This is intended for knocking out a gene and replacing it with Pmyo-2::GFP.
Proper citation: RRID:Addgene_91828 Copy
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