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Species: E. coli
Genetic Insert: PrhaBAD-sfGFP
Vector Backbone Description: Backbone Marker:DNA2.0; Backbone Size:3822; Vector Backbone:pJ404; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27247275
Proper citation: RRID:Addgene_87767 Copy
Species: E. coli
Genetic Insert: beta-gal
Vector Backbone Description: Backbone Size:10500; Vector Backbone:pUB23; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:3018930
Comments: Half life of beta-gal ~2 minutes. See author's map.
Proper citation: RRID:Addgene_8818 Copy
Species: E. coli
Genetic Insert: beta-gal
Vector Backbone Description: Backbone Size:10500; Vector Backbone:pUB23; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:3018930
Comments: Half life of beta-gal >20 hours. See author's map.
Proper citation: RRID:Addgene_8816 Copy
Species: E. coli
Genetic Insert: Streptavidin-K121R-Glutamate_Tag
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5400; Vector Backbone:pET21; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28757182
Comments: The residue numbering is based 1986 paper from Charles Cantor: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC339579 (see Figure 3)
In the original sequence, there is a signal peptide and N-terminal sequence that are proteolytically processed. Thus, the most common version is Core Streptavidin, which starts at residue 13 and ends at residue 139, as shown here: http://www.jbc.org/content/270/47/28204.long (see Figure 1)
Proper citation: RRID:Addgene_89880 Copy
Species: E. coli
Genetic Insert: Streptavidin-K80R-K121R-K132R-K134R-Glutamate_Tag
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5400; Vector Backbone:pET21; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28757182
Comments: The residue numbering is based 1986 paper from Charles Cantor: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC339579 (see Figure 3)
In the original sequence, there is a signal peptide and N-terminal sequence that are proteolytically processed. Thus, the most common version is Core Streptavidin, which starts at residue 13 and ends at residue 139, as shown here: http://www.jbc.org/content/270/47/28204.long (see Figure 1)
Proper citation: RRID:Addgene_89881 Copy
Species: E. coli
Genetic Insert: LacI-11/109
Vector Backbone Description: Backbone Marker: PMID: 3012611 with residual LacI C-terminal codons of pHG165 removed; Vector Backbone:phG165c; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22965134
Comments: WT LacI variant, stop codon inserted 11 amino acids from 3'-end of the gene; polymorphism at position 109 with T . The start codon of these genes is GTG, which is an alternative MET start codon in E. coli.
Proper citation: RRID:Addgene_90059 Copy
Species: E. coli
Genetic Insert: LacI/109
Vector Backbone Description: Backbone Marker: PMID: 3012611 with residual LacI C-terminal codons of pHG165 removed; Vector Backbone:phG165c; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22965134
Comments: WT LacI variant, polymorphism at position 109 with T . The start codon of these genes is GTG, which is an alternative MET start codon in E. coli.
Proper citation: RRID:Addgene_90058 Copy
Species: E. coli
Genetic Insert: FruR (CRA)
Vector Backbone Description: Backbone Marker:PMID: 3012611 with lacO1 binding site of pHG165 disabled by mutation; Vector Backbone:pHG165a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: WT FruR (CRA) . The start codon of these genes is GTG, which is an alternative MET start codon in E. coli.
Proper citation: RRID:Addgene_90066 Copy
Species: E. coli
Genetic Insert: Beta glucuronidase gene
Vector Backbone Description: Vector Backbone:pL0R; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32161816
Proper citation: RRID:Addgene_202137 Copy
Species: E. Coli
Genetic Insert: Hygromycin
Vector Backbone Description: Backbone Size:7944; Vector Backbone:pCHA1.1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37561850
Proper citation: RRID:Addgene_195510 Copy
Species: E. coli
Genetic Insert: CsgA
Vector Backbone Description: Backbone Size:2554; Vector Backbone:pZa; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36248312
Proper citation: RRID:Addgene_204503 Copy
Species: E. coli
Genetic Insert: broad-spectrum class A beta-lactamase TEM-116
Vector Backbone Description: Backbone Size:4743; Vector Backbone:pHI1; Vector Types:Bacterial Expression, Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_203880 Copy
Species: E. coli
Genetic Insert: monomeric red fluorescent protein
Vector Backbone Description: Backbone Size:4743; Vector Backbone:pHI1; Vector Types:Bacterial Expression, Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_203882 Copy
Species: E. coli
Genetic Insert: GFP-BIB H20A
Vector Backbone Description: Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37972066
Proper citation: RRID:Addgene_207593 Copy
Species: E. coli
Genetic Insert: TadA-RA4.2-SpCas9 D10A
Vector Backbone Description: Backbone Size:3402; Vector Backbone:pCMV with a BR322 origin; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38168987
Proper citation: RRID:Addgene_208307 Copy
Species: E. coli
Genetic Insert: TadA-RA4.3-SpCas9 D10A
Vector Backbone Description: Backbone Size:3402; Vector Backbone:pCMV with a BR322 origin; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38168987
Proper citation: RRID:Addgene_208308 Copy
Species: E. coli
Genetic Insert: TadA-RA3.2-SpCas9 D10A
Vector Backbone Description: Backbone Size:3402; Vector Backbone:pCMV with a BR322 origin; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38168987
Proper citation: RRID:Addgene_208303 Copy
Species: E. coli
Genetic Insert: TadA-RA3.3-SpCas9 D10A
Vector Backbone Description: Backbone Size:3402; Vector Backbone:pCMV with a BR322 origin; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38168987
Proper citation: RRID:Addgene_208304 Copy
Species: E. coli
Genetic Insert: TadA-RA5.3-SpCas9 D10A
Vector Backbone Description: Backbone Size:3402; Vector Backbone:pCMV with a BR322 origin; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38168987
Proper citation: RRID:Addgene_208314 Copy
Species: E. coli
Genetic Insert: TadA-RA5.5-SpCas9 D10A
Vector Backbone Description: Backbone Size:3402; Vector Backbone:pCMV with a BR322 origin; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38168987
Proper citation: RRID:Addgene_208316 Copy
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