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pCK301
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_87767 PrhaBAD-sfGFP E. coli Ampicillin PMID:27247275 Backbone Marker:DNA2.0; Backbone Size:3822; Vector Backbone:pJ404; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-15 01:21:47 1
pUB23-R-beta-gal
 
Resource Report
Resource Website 		RRID:Addgene_8818 beta-gal E. coli Ampicillin PMID:3018930 Half life of beta-gal ~2 minutes. See author's map. Backbone Size:10500; Vector Backbone:pUB23; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin R (arginine) at amino terminus of beta-gal 2026-08-15 01:21:50 0
pUB23-M-beta-gal
 
Resource Report
Resource Website 		RRID:Addgene_8816 beta-gal E. coli Ampicillin PMID:3018930 Half life of beta-gal >20 hours. See author's map. Backbone Size:10500; Vector Backbone:pUB23; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin M (methionine) at amino terminus of beta-gal 2026-08-15 01:21:50 0
pET21-Streptavidin-K121R
 
Resource Report
Resource Website 		RRID:Addgene_89880 Streptavidin-K121R-Glutamate_Tag E. coli Ampicillin PMID:28757182 The residue numbering is based 1986 paper from Charles Cantor: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC339579 (see Figure 3) In the original sequence, there is a signal peptide and N-terminal sequence that are proteolytically processed. Thus, the most common version is Core Streptavidin, which starts at residue 13 and ends at residue 139, as shown here: http://www.jbc.org/content/270/47/28204.long (see Figure 1) Backbone Marker:Novagen; Backbone Size:5400; Vector Backbone:pET21; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin K121R 2026-08-15 01:22:01 0
pET21-Flavidin
 
Resource Report
Resource Website 		RRID:Addgene_89881 Streptavidin-K80R-K121R-K132R-K134R-Glutamate_Tag E. coli Ampicillin PMID:28757182 The residue numbering is based 1986 paper from Charles Cantor: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC339579 (see Figure 3) In the original sequence, there is a signal peptide and N-terminal sequence that are proteolytically processed. Thus, the most common version is Core Streptavidin, which starts at residue 13 and ends at residue 139, as shown here: http://www.jbc.org/content/270/47/28204.long (see Figure 1) Backbone Marker:Novagen; Backbone Size:5400; Vector Backbone:pET21; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin K80R, K121R, K132R, K134R 2026-08-15 01:22:01 0
LacI-11/109-pHG165c
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_90059 LacI-11/109 E. coli Ampicillin PMID:22965134 WT LacI variant, stop codon inserted 11 amino acids from 3'-end of the gene; polymorphism at position 109 with T . The start codon of these genes is GTG, which is an alternative MET start codon in E. coli. Backbone Marker: PMID: 3012611 with residual LacI C-terminal codons of pHG165 removed; Vector Backbone:phG165c; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 109 2026-08-15 01:22:03 1
LacI/109-pHG165c
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_90058 LacI/109 E. coli Ampicillin PMID:22965134 WT LacI variant, polymorphism at position 109 with T . The start codon of these genes is GTG, which is an alternative MET start codon in E. coli. Backbone Marker: PMID: 3012611 with residual LacI C-terminal codons of pHG165 removed; Vector Backbone:phG165c; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 109 2026-08-15 01:22:03 1
FruR-pHG165a (CRA)
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_90066 FruR (CRA) E. coli Ampicillin WT FruR (CRA) . The start codon of these genes is GTG, which is an alternative MET start codon in E. coli. Backbone Marker:PMID: 3012611 with lacO1 binding site of pHG165 disabled by mutation; Vector Backbone:pHG165a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:22:03 2
pL0_CD-GUS
 
Resource Report
Resource Website 		RRID:Addgene_202137 Beta glucuronidase gene E. coli Spectinomycin PMID:32161816 Vector Backbone:pL0R; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin None 2026-08-15 01:27:36 0
pCHA1.1-Rap2a-V5-Hygro
 
Resource Report
Resource Website 		RRID:Addgene_195510 Hygromycin E. Coli Ampicillin PMID:37561850 Backbone Size:7944; Vector Backbone:pCHA1.1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin 2026-08-15 01:28:52 0
pZa pLtetO CsgA-SpyTag
 
Resource Report
Resource Website 		RRID:Addgene_204503 CsgA E. coli Ampicillin PMID:36248312 Backbone Size:2554; Vector Backbone:pZa; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-15 01:28:19 0
pHflu2
 
Resource Report
Resource Website 		RRID:Addgene_203880 broad-spectrum class A beta-lactamase TEM-116 E. coli Chloramphenicol and Ampicillin Backbone Size:4743; Vector Backbone:pHI1; Vector Types:Bacterial Expression, Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol and Ampicillin 2026-08-15 01:28:23 0
pHflu4
 
Resource Report
Resource Website 		RRID:Addgene_203882 monomeric red fluorescent protein E. coli Chloramphenicol Backbone Size:4743; Vector Backbone:pHI1; Vector Types:Bacterial Expression, Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol 2026-08-15 01:28:23 0
pJS124
 
Resource Report
Resource Website 		RRID:Addgene_207593 GFP-BIB H20A E. coli Ampicillin PMID:37972066 Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:28:22 0
ABE-RA4.2
 
Resource Report
Resource Website 		RRID:Addgene_208307 TadA-RA4.2-SpCas9 D10A E. coli Ampicillin PMID:38168987 Backbone Size:3402; Vector Backbone:pCMV with a BR322 origin; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin P48A, R51H, I76F, A106V, D108G, K110R, T111H, D119N, H123Y, M126I, N127K, S146C, D147R, R152P, Q154R, E155V, I156F 2026-08-15 01:28:29 0
ABE-RA4.3
 
Resource Report
Resource Website 		RRID:Addgene_208308 TadA-RA4.3-SpCas9 D10A E. coli Ampicillin PMID:38168987 Backbone Size:3402; Vector Backbone:pCMV with a BR322 origin; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin P48A, R51H, I76F, A106V, D108G, K110R, T111H, D119N, H123Y, M126I, N127K, D147R, R152P, Q154R, E155V, I156F, K157N 2026-08-15 01:28:29 0
ABE-RA3.2
 
Resource Report
Resource Website 		RRID:Addgene_208303 TadA-RA3.2-SpCas9 D10A E. coli Ampicillin PMID:38168987 Backbone Size:3402; Vector Backbone:pCMV with a BR322 origin; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin E27D, P48A, R51H, D108G, K110R, A114V, H122R, M126I, N127K 2026-08-15 01:28:29 0
ABE-RA3.3
 
Resource Report
Resource Website 		RRID:Addgene_208304 TadA-RA3.3-SpCas9 D10A E. coli Ampicillin PMID:38168987 Backbone Size:3402; Vector Backbone:pCMV with a BR322 origin; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin E27D, R47K, P48A, R51H, I76F, D108G, K110R, A114V, H122R, M126I, N127K 2026-08-15 01:28:29 0
ABE-RA5.3
 
Resource Report
Resource Website 		RRID:Addgene_208314 TadA-RA5.3-SpCas9 D10A E. coli Ampicillin PMID:38168987 Backbone Size:3402; Vector Backbone:pCMV with a BR322 origin; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin W23R, H36L, R47K, P48A, R51L, I76F, V82S, A106V, D108G, A109S, K110R, T111H, A114V, D119N, H123Y, M1216I, N127K, D147R, R152P, Q154R, E155V, I156F 2026-08-15 01:28:29 0
ABE-RA5.5
 
Resource Report
Resource Website 		RRID:Addgene_208316 TadA-RA5.5-SpCas9 D10A E. coli Ampicillin PMID:38168987 Backbone Size:3402; Vector Backbone:pCMV with a BR322 origin; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin W23R, H36L, R47K, P48A, R51L, I76Y, V82S, A106V, D108G, K110R, T111H, A114V, D119N, H123Y, M1216I, N127K, S146C, D147R, R152P, Q154R, E155V, I156F, K157N, K161N, T166I, D167N 2026-08-15 01:28:29 0

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